Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang...

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Autores principales:	Zhang PF, Bao Y, Draz MS, Lu HQ, Liu C, Han HX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a4821def47849a7838fd3202f5176a5
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spelling	oai:doaj.org-article:0a4821def47849a7838fd3202f5176a52021-12-02T02:31:41ZRapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay1178-2013https://doaj.org/article/0a4821def47849a7838fd3202f5176a52015-09-01T00:00:00Zhttps://www.dovepress.com/rapid-and-quantitative-detection-of-c-reactive-protein-based-on-quantu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Faculty of Science, Tanta University, Tanta, Egypt*These authors contributed equally to this workAbstract: Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.Keywords: C-reactive proteins, point-of-care test, Glutathione capped QDs, PEGylationZhang PFBao YDraz MSLu HQLiu CHan HXDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6161-6173 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang PF Bao Y Draz MS Lu HQ Liu C Han HX Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
description	Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Faculty of Science, Tanta University, Tanta, Egypt*These authors contributed equally to this workAbstract: Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.Keywords: C-reactive proteins, point-of-care test, Glutathione capped QDs, PEGylation
format	article
author	Zhang PF Bao Y Draz MS Lu HQ Liu C Han HX
author_facet	Zhang PF Bao Y Draz MS Lu HQ Liu C Han HX
author_sort	Zhang PF
title	Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
title_short	Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
title_full	Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
title_fullStr	Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
title_full_unstemmed	Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
title_sort	rapid and quantitative detection of c-reactive protein based on quantum dots and immunofiltration assay
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/0a4821def47849a7838fd3202f5176a5
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Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

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