A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy

The simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component...

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Autores principales:	Ranjan Rajeev, Ferrara Maria Antonietta, Sirleto Luigi
Formato:	article
Lenguaje:	EN
Publicado:	EDP Sciences 2021
Materias:	Physics QC1-999
Acceso en línea:	https://doaj.org/article/0a482a9f34024931ba69402f8af98b52
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spelling	oai:doaj.org-article:0a482a9f34024931ba69402f8af98b522021-12-02T17:12:51ZA Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy2100-014X10.1051/epjconf/202125511009https://doaj.org/article/0a482a9f34024931ba69402f8af98b522021-01-01T00:00:00Zhttps://www.epj-conferences.org/articles/epjconf/pdf/2021/09/epjconf_eosam2021_11009.pdfhttps://doaj.org/toc/2100-014XThe simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component-based system due to the linear dependence of signals on concentration and its chemical selectivity. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, plays a fundamental role. Here in this work, the cross-correlation of Ti:Sa & OPO femtosecond laser beams in a stimulated Raman scattering microscope is measured. The separation between protein and lipid bands in the C-H region is important for biochemical research and is successfully classified.Ranjan RajeevFerrara Maria AntoniettaSirleto LuigiEDP SciencesarticlePhysicsQC1-999ENEPJ Web of Conferences, Vol 255, p 11009 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physics QC1-999
spellingShingle	Physics QC1-999 Ranjan Rajeev Ferrara Maria Antonietta Sirleto Luigi A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
description	The simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component-based system due to the linear dependence of signals on concentration and its chemical selectivity. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, plays a fundamental role. Here in this work, the cross-correlation of Ti:Sa & OPO femtosecond laser beams in a stimulated Raman scattering microscope is measured. The separation between protein and lipid bands in the C-H region is important for biochemical research and is successfully classified.
format	article
author	Ranjan Rajeev Ferrara Maria Antonietta Sirleto Luigi
author_facet	Ranjan Rajeev Ferrara Maria Antonietta Sirleto Luigi
author_sort	Ranjan Rajeev
title	A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
title_short	A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
title_full	A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
title_fullStr	A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
title_full_unstemmed	A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
title_sort	spectral resolution study in femtosecond stimulated raman scattering microscopy
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/0a482a9f34024931ba69402f8af98b52
work_keys_str_mv	AT ranjanrajeev aspectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy AT ferraramariaantonietta aspectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy AT sirletoluigi aspectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy AT ranjanrajeev spectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy AT ferraramariaantonietta spectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy AT sirletoluigi spectralresolutionstudyinfemtosecondstimulatedramanscatteringmicroscopy
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A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy

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