Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening

SCRaMbLE can lead to great genetic diversity for product biosynthesis but is limited by screening methods. Here the authors develop a rapid workflow using automation, ultra-fast LC/MS and barcoded nanopore sequencing to identify best performing strains.

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Autores principales:	G.-O. F. Gowers, S. M. Chee, D. Bell, L. Suckling, M. Kern, D. Tew, D. W. McClymont, T. Ellis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0a521b41817846ec8416988a01e7bb8f
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spelling	oai:doaj.org-article:0a521b41817846ec8416988a01e7bb8f2021-12-02T15:37:12ZImproved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening10.1038/s41467-020-14708-z2041-1723https://doaj.org/article/0a521b41817846ec8416988a01e7bb8f2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14708-zhttps://doaj.org/toc/2041-1723SCRaMbLE can lead to great genetic diversity for product biosynthesis but is limited by screening methods. Here the authors develop a rapid workflow using automation, ultra-fast LC/MS and barcoded nanopore sequencing to identify best performing strains.G.-O. F. GowersS. M. CheeD. BellL. SucklingM. KernD. TewD. W. McClymontT. EllisNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q G.-O. F. Gowers S. M. Chee D. Bell L. Suckling M. Kern D. Tew D. W. McClymont T. Ellis Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
description	SCRaMbLE can lead to great genetic diversity for product biosynthesis but is limited by screening methods. Here the authors develop a rapid workflow using automation, ultra-fast LC/MS and barcoded nanopore sequencing to identify best performing strains.
format	article
author	G.-O. F. Gowers S. M. Chee D. Bell L. Suckling M. Kern D. Tew D. W. McClymont T. Ellis
author_facet	G.-O. F. Gowers S. M. Chee D. Bell L. Suckling M. Kern D. Tew D. W. McClymont T. Ellis
author_sort	G.-O. F. Gowers
title	Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
title_short	Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
title_full	Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
title_fullStr	Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
title_full_unstemmed	Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
title_sort	improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid lc-ms screening
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0a521b41817846ec8416988a01e7bb8f
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Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening

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