Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening
SCRaMbLE can lead to great genetic diversity for product biosynthesis but is limited by screening methods. Here the authors develop a rapid workflow using automation, ultra-fast LC/MS and barcoded nanopore sequencing to identify best performing strains.
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0a521b41817846ec8416988a01e7bb8f |
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