Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production

Abstract A key challenge to advance the efficiency of bioprocesses is the uncoupling of biomass from product formation, as biomass represents a by-product that is in most cases difficult to recycle efficiently. Using the example of rhamnolipid biosurfactants, a temperature-sensitive heterologous pro...

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Autores principales:	Philipp Noll, Chantal Treinen, Sven Müller, Lars Lilge, Rudolf Hausmann, Marius Henkel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a534d1ef0734290b96a3aec40a84d8e
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spelling	oai:doaj.org-article:0a534d1ef0734290b96a3aec40a84d8e2021-12-02T17:55:04ZExploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production10.1038/s41598-021-94400-42045-2322https://doaj.org/article/0a534d1ef0734290b96a3aec40a84d8e2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94400-4https://doaj.org/toc/2045-2322Abstract A key challenge to advance the efficiency of bioprocesses is the uncoupling of biomass from product formation, as biomass represents a by-product that is in most cases difficult to recycle efficiently. Using the example of rhamnolipid biosurfactants, a temperature-sensitive heterologous production system under translation control of a fourU RNA thermometer from Salmonella was established to allow separating phases of preferred growth from product formation. Rhamnolipids as bulk chemicals represent a model system for future processes of industrial biotechnology and are therefore tied to the efficiency requirements in competition with the chemical industry. Experimental data confirms function of the RNA thermometer and suggests a major effect of temperature on specific rhamnolipid production rates with an increase of the average production rate by a factor of 11 between 25 and 38 °C, while the major part of this increase is attributable to the regulatory effect of the RNA thermometer rather than an unspecific overall increase in bacterial metabolism. The production capacity of the developed temperature sensitive-system was evaluated in a simple batch process driven by a temperature switch. Product formation was evaluated by efficiency parameters and yields, confirming increased product formation rates and product-per-biomass yields compared to a high titer heterologous rhamnolipid production process from literature.Philipp NollChantal TreinenSven MüllerLars LilgeRudolf HausmannMarius HenkelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Philipp Noll Chantal Treinen Sven Müller Lars Lilge Rudolf Hausmann Marius Henkel Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
description	Abstract A key challenge to advance the efficiency of bioprocesses is the uncoupling of biomass from product formation, as biomass represents a by-product that is in most cases difficult to recycle efficiently. Using the example of rhamnolipid biosurfactants, a temperature-sensitive heterologous production system under translation control of a fourU RNA thermometer from Salmonella was established to allow separating phases of preferred growth from product formation. Rhamnolipids as bulk chemicals represent a model system for future processes of industrial biotechnology and are therefore tied to the efficiency requirements in competition with the chemical industry. Experimental data confirms function of the RNA thermometer and suggests a major effect of temperature on specific rhamnolipid production rates with an increase of the average production rate by a factor of 11 between 25 and 38 °C, while the major part of this increase is attributable to the regulatory effect of the RNA thermometer rather than an unspecific overall increase in bacterial metabolism. The production capacity of the developed temperature sensitive-system was evaluated in a simple batch process driven by a temperature switch. Product formation was evaluated by efficiency parameters and yields, confirming increased product formation rates and product-per-biomass yields compared to a high titer heterologous rhamnolipid production process from literature.
format	article
author	Philipp Noll Chantal Treinen Sven Müller Lars Lilge Rudolf Hausmann Marius Henkel
author_facet	Philipp Noll Chantal Treinen Sven Müller Lars Lilge Rudolf Hausmann Marius Henkel
author_sort	Philipp Noll
title	Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
title_short	Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
title_full	Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
title_fullStr	Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
title_full_unstemmed	Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
title_sort	exploiting rna thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0a534d1ef0734290b96a3aec40a84d8e
work_keys_str_mv	AT philippnoll exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction AT chantaltreinen exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction AT svenmuller exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction AT larslilge exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction AT rudolfhausmann exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction AT mariushenkel exploitingrnathermometerdrivenmolecularbioprocesscontrolasaconceptforheterologousrhamnolipidproduction
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Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production

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