Interleukin-1 exerts distinct actions on different cell types of the brain in vitro

Ying An, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Interleukin-1 (IL-1) is a critical neuroinflammatory mediator in the central nervous system (CNS). In this study, we investigated the effect of IL-1 on inducing inflammation-related gene express...

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Autores principales:	Ying An, Qun Chen, Ning Quan
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0a595fb3bd5141ec894022c54d3ed3a6
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spelling	oai:doaj.org-article:0a595fb3bd5141ec894022c54d3ed3a62021-12-02T02:37:02ZInterleukin-1 exerts distinct actions on different cell types of the brain in vitro1178-7031https://doaj.org/article/0a595fb3bd5141ec894022c54d3ed3a62011-01-01T00:00:00Zhttp://www.dovepress.com/interleukin-1exerts-distinct-actions-on-different-cell-types-of-the-br-a6059https://doaj.org/toc/1178-7031Ying An, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Interleukin-1 (IL-1) is a critical neuroinflammatory mediator in the central nervous system (CNS). In this study, we investigated the effect of IL-1 on inducing inflammation-related gene expression in three astrocyte, two microglial, and one brain endothelial cell line. Interleukin-1 beta (IL-1&beta;) is found to be produced by the two microglial cell lines constitutively, but these cells do not respond to IL-1&beta; stimulation. The three astrocyte cell lines responded to IL-1&szlig; stimulation by expressing MCP-1, CXCL-1, and VCAM-1, but different subtypes of astrocytes exhibited different expression profiles after IL-1&beta; stimulation. The brain endothelial cells showed strongest response to IL-1&beta; by producing MCP-1, CXCL-1, VCAM-1, ICAM-1, IL-6, and COX-2 mRNA. The induction of endothelial COX-2 mRNA is shown to be mediated by p38 MAPK pathway, whereas the induction of other genes is mediated by the NF-&kappa;B pathway. These results demonstrate that IL-1 exerts distinct cell type-specific action in CNS cells and suggest that IL-1-mediated neuroinflammation is the result of the summation of multiple responses from different cell types in the CNS to IL-1.Keywords: astrocyte, microglia, endothelial cells, signal transduction pathways, gene expression&nbsp; Ying AnQun ChenNing QuanDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2011, Iss default, Pp 11-20 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Ying An Qun Chen Ning Quan Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
description	Ying An, Qun Chen, Ning QuanDepartment of Oral Biology, Ohio State University, Columbus, OH, USAAbstract: Interleukin-1 (IL-1) is a critical neuroinflammatory mediator in the central nervous system (CNS). In this study, we investigated the effect of IL-1 on inducing inflammation-related gene expression in three astrocyte, two microglial, and one brain endothelial cell line. Interleukin-1 beta (IL-1&beta;) is found to be produced by the two microglial cell lines constitutively, but these cells do not respond to IL-1&beta; stimulation. The three astrocyte cell lines responded to IL-1&szlig; stimulation by expressing MCP-1, CXCL-1, and VCAM-1, but different subtypes of astrocytes exhibited different expression profiles after IL-1&beta; stimulation. The brain endothelial cells showed strongest response to IL-1&beta; by producing MCP-1, CXCL-1, VCAM-1, ICAM-1, IL-6, and COX-2 mRNA. The induction of endothelial COX-2 mRNA is shown to be mediated by p38 MAPK pathway, whereas the induction of other genes is mediated by the NF-&kappa;B pathway. These results demonstrate that IL-1 exerts distinct cell type-specific action in CNS cells and suggest that IL-1-mediated neuroinflammation is the result of the summation of multiple responses from different cell types in the CNS to IL-1.Keywords: astrocyte, microglia, endothelial cells, signal transduction pathways, gene expression&nbsp;
format	article
author	Ying An Qun Chen Ning Quan
author_facet	Ying An Qun Chen Ning Quan
author_sort	Ying An
title	Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
title_short	Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
title_full	Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
title_fullStr	Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
title_full_unstemmed	Interleukin-1 exerts distinct actions on different cell types of the brain in vitro
title_sort	interleukin-1 exerts distinct actions on different cell types of the brain in vitro
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/0a595fb3bd5141ec894022c54d3ed3a6
work_keys_str_mv	AT yingan interleukin1exertsdistinctactionsondifferentcelltypesofthebraininvitro AT qunchen interleukin1exertsdistinctactionsondifferentcelltypesofthebraininvitro AT ningquan interleukin1exertsdistinctactionsondifferentcelltypesofthebraininvitro
_version_	1718402327418765312

Interleukin-1 exerts distinct actions on different cell types of the brain in vitro

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