CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe

Abstract CVD 103-HgR live, attenuated oral cholera vaccine strain is indicated for single dose immunization against Vibrio cholerae, the causative agent for cholera. The vaccine packets containing buffer powder and lyophilized CVD 103-HgR are reconstituted in water and consumed. Studies were perform...

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Autores principales:	R. Paul Duffin, Michael Delbuono, Kylie Nishioka, Paul Shabram, Amish A. Patel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a5e5dc3a4e647ec8f0538435b172ab6
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spelling	oai:doaj.org-article:0a5e5dc3a4e647ec8f0538435b172ab62021-12-02T17:12:25ZCVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe10.1038/s41598-021-92182-32045-2322https://doaj.org/article/0a5e5dc3a4e647ec8f0538435b172ab62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92182-3https://doaj.org/toc/2045-2322Abstract CVD 103-HgR live, attenuated oral cholera vaccine strain is indicated for single dose immunization against Vibrio cholerae, the causative agent for cholera. The vaccine packets containing buffer powder and lyophilized CVD 103-HgR are reconstituted in water and consumed. Studies were performed to explore the viability of CVD 103-HgR in drinking waters from common sources. CVD 103-HgR vaccine was reconstituted in bottled and tap waters from the United States and Europe, and viability was measured via colony forming units assay. Chemical analysis of select water samples was used to identify chemicals that have a negative effect on CVD 103-HgR viability. CVD 103-HgR titers were stable in all bottled waters tested, including purified bottled water, bottled spring water, and sparkling waters. However, tap water from certain cities in the US and Europe affected viability and are not compatible with vaccine. Water chemistry revealed that these tap waters contained copper, likely leached from copper plumbing. These studies give high confidence in the stability of CVD 103-HgR reconstituted in a variety of bottled waters. Waters containing copper, including tap water, should not be used to reconstitute CVD 103-HgR strain oral vaccine due to the common use of copper plumbing.R. Paul DuffinMichael DelbuonoKylie NishiokaPaul ShabramAmish A. PatelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. Paul Duffin Michael Delbuono Kylie Nishioka Paul Shabram Amish A. Patel CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
description	Abstract CVD 103-HgR live, attenuated oral cholera vaccine strain is indicated for single dose immunization against Vibrio cholerae, the causative agent for cholera. The vaccine packets containing buffer powder and lyophilized CVD 103-HgR are reconstituted in water and consumed. Studies were performed to explore the viability of CVD 103-HgR in drinking waters from common sources. CVD 103-HgR vaccine was reconstituted in bottled and tap waters from the United States and Europe, and viability was measured via colony forming units assay. Chemical analysis of select water samples was used to identify chemicals that have a negative effect on CVD 103-HgR viability. CVD 103-HgR titers were stable in all bottled waters tested, including purified bottled water, bottled spring water, and sparkling waters. However, tap water from certain cities in the US and Europe affected viability and are not compatible with vaccine. Water chemistry revealed that these tap waters contained copper, likely leached from copper plumbing. These studies give high confidence in the stability of CVD 103-HgR reconstituted in a variety of bottled waters. Waters containing copper, including tap water, should not be used to reconstitute CVD 103-HgR strain oral vaccine due to the common use of copper plumbing.
format	article
author	R. Paul Duffin Michael Delbuono Kylie Nishioka Paul Shabram Amish A. Patel
author_facet	R. Paul Duffin Michael Delbuono Kylie Nishioka Paul Shabram Amish A. Patel
author_sort	R. Paul Duffin
title	CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
title_short	CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
title_full	CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
title_fullStr	CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
title_full_unstemmed	CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe
title_sort	cvd 103-hgr live, attenuated cholera vaccine strain viability in drinking waters from the us and europe
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0a5e5dc3a4e647ec8f0538435b172ab6
work_keys_str_mv	AT rpaulduffin cvd103hgrliveattenuatedcholeravaccinestrainviabilityindrinkingwatersfromtheusandeurope AT michaeldelbuono cvd103hgrliveattenuatedcholeravaccinestrainviabilityindrinkingwatersfromtheusandeurope AT kylienishioka cvd103hgrliveattenuatedcholeravaccinestrainviabilityindrinkingwatersfromtheusandeurope AT paulshabram cvd103hgrliveattenuatedcholeravaccinestrainviabilityindrinkingwatersfromtheusandeurope AT amishapatel cvd103hgrliveattenuatedcholeravaccinestrainviabilityindrinkingwatersfromtheusandeurope
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CVD 103-HgR live, attenuated cholera vaccine strain viability in drinking waters from the US and Europe

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