GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma

BackgroundGlucose-6-phosphate isomerase (GPI) plays an important role in glycolysis and gluconeogenesis. However, the role of GPI in lung adenocarcinoma (LUAD) remains unclear.MethodsAll original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and...

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Autores principales:	Jiahui Han, Xinzhou Deng, Renhuang Sun, Ming Luo, Meng Liang, Bing Gu, Te Zhang, Zhen Peng, Ying Lu, Chao Tian, Yutao Yan, Zhiguo Luo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glucose-6-phosphate isomerase (GPI) prognosis (carcinoma) immune infiltrate cell cycle lung adenocarcinoma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/0a60eef943844d7ab2bb2f68b63ecc13
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spelling	oai:doaj.org-article:0a60eef943844d7ab2bb2f68b63ecc132021-12-01T12:23:52ZGPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma2234-943X10.3389/fonc.2021.752642https://doaj.org/article/0a60eef943844d7ab2bb2f68b63ecc132021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.752642/fullhttps://doaj.org/toc/2234-943XBackgroundGlucose-6-phosphate isomerase (GPI) plays an important role in glycolysis and gluconeogenesis. However, the role of GPI in lung adenocarcinoma (LUAD) remains unclear.MethodsAll original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and integrated via R 3.2.2. GPI expression was explored with TCGA, GEO, and Oncomine databases. Immunohistochemistry staining was used to analyze GPI expression in clinical specimens. The correlations between GPI and cancer immune characteristics were analyzed via the TIMER and TISIDB databases. GPI-specific siRNAs were used to verify the role of GPI expression on cell proliferation and cell cycle distribution.ResultsIn general, GPI is predominantly overexpressed and has reference value in the diagnosis and prognostic estimation of LUAD. Upregulated GPI was associated with poorer overall survival, clinical stage, N stage, and primary therapy outcome in LUAD. Mechanistically, we identified a hub gene that included a total of 56 GPI-related genes, which were tightly associated with the cell cycle pathway in LUAD patients. Knockdown of GPI induced cell proliferation inhibition and cell cycle arrest. GPI expression was positively correlated with infiltrating levels of Th2 cells and regulatory T cells (Tregs); in contrast, GPI expression was negatively correlated with infiltrating levels of CD8+ T cells, central memory T cells, dendritic cells, macrophages, mast cells, and eosinophils. GPI was negatively correlated with the expression of immunostimulators, such as CD40L, IL6R, and TMEM173, in LUAD.ConclusionGPI may play an important role in the cell cycle and can be used as a prognostic biomarker for determining the prognosis and immune infiltration in LUAD.Jiahui HanJiahui HanXinzhou DengXinzhou DengRenhuang SunMing LuoMeng LiangBing GuTe ZhangZhen PengYing LuChao TianYutao YanZhiguo LuoZhiguo LuoFrontiers Media S.A.articleglucose-6-phosphate isomerase (GPI)prognosis (carcinoma)immune infiltratecell cyclelung adenocarcinomaNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glucose-6-phosphate isomerase (GPI) prognosis (carcinoma) immune infiltrate cell cycle lung adenocarcinoma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	glucose-6-phosphate isomerase (GPI) prognosis (carcinoma) immune infiltrate cell cycle lung adenocarcinoma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Jiahui Han Jiahui Han Xinzhou Deng Xinzhou Deng Renhuang Sun Ming Luo Meng Liang Bing Gu Te Zhang Zhen Peng Ying Lu Chao Tian Yutao Yan Zhiguo Luo Zhiguo Luo GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
description	BackgroundGlucose-6-phosphate isomerase (GPI) plays an important role in glycolysis and gluconeogenesis. However, the role of GPI in lung adenocarcinoma (LUAD) remains unclear.MethodsAll original data were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and integrated via R 3.2.2. GPI expression was explored with TCGA, GEO, and Oncomine databases. Immunohistochemistry staining was used to analyze GPI expression in clinical specimens. The correlations between GPI and cancer immune characteristics were analyzed via the TIMER and TISIDB databases. GPI-specific siRNAs were used to verify the role of GPI expression on cell proliferation and cell cycle distribution.ResultsIn general, GPI is predominantly overexpressed and has reference value in the diagnosis and prognostic estimation of LUAD. Upregulated GPI was associated with poorer overall survival, clinical stage, N stage, and primary therapy outcome in LUAD. Mechanistically, we identified a hub gene that included a total of 56 GPI-related genes, which were tightly associated with the cell cycle pathway in LUAD patients. Knockdown of GPI induced cell proliferation inhibition and cell cycle arrest. GPI expression was positively correlated with infiltrating levels of Th2 cells and regulatory T cells (Tregs); in contrast, GPI expression was negatively correlated with infiltrating levels of CD8+ T cells, central memory T cells, dendritic cells, macrophages, mast cells, and eosinophils. GPI was negatively correlated with the expression of immunostimulators, such as CD40L, IL6R, and TMEM173, in LUAD.ConclusionGPI may play an important role in the cell cycle and can be used as a prognostic biomarker for determining the prognosis and immune infiltration in LUAD.
format	article
author	Jiahui Han Jiahui Han Xinzhou Deng Xinzhou Deng Renhuang Sun Ming Luo Meng Liang Bing Gu Te Zhang Zhen Peng Ying Lu Chao Tian Yutao Yan Zhiguo Luo Zhiguo Luo
author_facet	Jiahui Han Jiahui Han Xinzhou Deng Xinzhou Deng Renhuang Sun Ming Luo Meng Liang Bing Gu Te Zhang Zhen Peng Ying Lu Chao Tian Yutao Yan Zhiguo Luo Zhiguo Luo
author_sort	Jiahui Han
title	GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
title_short	GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
title_full	GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
title_fullStr	GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
title_full_unstemmed	GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma
title_sort	gpi is a prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0a60eef943844d7ab2bb2f68b63ecc13
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GPI Is a Prognostic Biomarker and Correlates With Immune Infiltrates in Lung Adenocarcinoma

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