The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme

Background: Chemotherapy drugs are not effective in the treatment of primary brain tumors due to the low efficacy of these drugs and drug transfer from the blood-brain barrier (BBB) toward the tumor site. Our purpose in this study was to assess the co-delivery of anticancer drugs to increase drug pe...

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Autores principales:	Mansoureh Hashemi, Alireza Zali
Formato:	article
Lenguaje:	EN
Publicado:	Shahid Beheshti University of Medical Sciences 2020
Materias:	paclitaxel methotrexate pegylated nanoparticle plga drugs co-delivery synergistic effect apoptosis glioblastoma multiforme anticancer drugs Medicine R
Acceso en línea:	https://doaj.org/article/0a6522dd64b84330b78eae7537ac6911
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spelling	oai:doaj.org-article:0a6522dd64b84330b78eae7537ac69112021-11-16T11:06:27ZThe Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme2383-18712383-209610.34172/icnj.2020.23https://doaj.org/article/0a6522dd64b84330b78eae7537ac69112020-10-01T00:00:00Zhttps://journals.sbmu.ac.ir/neuroscience/article/view/31302/25812https://doaj.org/toc/2383-1871https://doaj.org/toc/2383-2096Background: Chemotherapy drugs are not effective in the treatment of primary brain tumors due to the low efficacy of these drugs and drug transfer from the blood-brain barrier (BBB) toward the tumor site. Our purpose in this study was to assess the co-delivery of anticancer drugs to increase drug permeability from BBB. Methods: In this study, two chemotherapy drugs, namely methotrexate (MTX) and paclitaxel (PTX), were inserted into polyvinyl alcohol and poloxamer188-conjugated nanoparticles (NPs). Astrocytes were treated with different concentrations of 0-50 µg/ml from MTX, PTX, the MTX-PTX mixture, PTX-loaded NPs, MTX-loaded NPs, and PTX-MTX co-loaded NPs for 48 hours. The tumoricidal effect was assessed using the survival rate, Hoechst staining, and western blotting. Results: The results indicated significant reduction of the survival rate in astrocytes treated with PTX-MTX co-loaded NPs. In addition, apoptosis hallmarks consisting of fragmented DNA, overexpression of Bax, and expression reduction of Bcl-2 were in the cultured astrocytes. Conclusions: Our study proposes that the PTX-MTX co-delivery to NPs could be used as a possible approach for anti-cancer drug delivery to glioblastoma multiforme.Mansoureh HashemiAlireza ZaliShahid Beheshti University of Medical Sciencesarticlepaclitaxelmethotrexatepegylated nanoparticleplgadrugs co-deliverysynergistic effectapoptosisglioblastoma multiformeanticancer drugsMedicineRENInternational Clinical Neuroscience Journal, Vol 7, Iss 4, Pp 185-189 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	paclitaxel methotrexate pegylated nanoparticle plga drugs co-delivery synergistic effect apoptosis glioblastoma multiforme anticancer drugs Medicine R
spellingShingle	paclitaxel methotrexate pegylated nanoparticle plga drugs co-delivery synergistic effect apoptosis glioblastoma multiforme anticancer drugs Medicine R Mansoureh Hashemi Alireza Zali The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
description	Background: Chemotherapy drugs are not effective in the treatment of primary brain tumors due to the low efficacy of these drugs and drug transfer from the blood-brain barrier (BBB) toward the tumor site. Our purpose in this study was to assess the co-delivery of anticancer drugs to increase drug permeability from BBB. Methods: In this study, two chemotherapy drugs, namely methotrexate (MTX) and paclitaxel (PTX), were inserted into polyvinyl alcohol and poloxamer188-conjugated nanoparticles (NPs). Astrocytes were treated with different concentrations of 0-50 µg/ml from MTX, PTX, the MTX-PTX mixture, PTX-loaded NPs, MTX-loaded NPs, and PTX-MTX co-loaded NPs for 48 hours. The tumoricidal effect was assessed using the survival rate, Hoechst staining, and western blotting. Results: The results indicated significant reduction of the survival rate in astrocytes treated with PTX-MTX co-loaded NPs. In addition, apoptosis hallmarks consisting of fragmented DNA, overexpression of Bax, and expression reduction of Bcl-2 were in the cultured astrocytes. Conclusions: Our study proposes that the PTX-MTX co-delivery to NPs could be used as a possible approach for anti-cancer drug delivery to glioblastoma multiforme.
format	article
author	Mansoureh Hashemi Alireza Zali
author_facet	Mansoureh Hashemi Alireza Zali
author_sort	Mansoureh Hashemi
title	The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
title_short	The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
title_full	The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
title_fullStr	The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
title_full_unstemmed	The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme
title_sort	synergistic effect of co-delivery of anticancer drugs into astrocytes isolated from human glioblastoma multiforme
publisher	Shahid Beheshti University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/0a6522dd64b84330b78eae7537ac6911
work_keys_str_mv	AT mansourehhashemi thesynergisticeffectofcodeliveryofanticancerdrugsintoastrocytesisolatedfromhumanglioblastomamultiforme AT alirezazali thesynergisticeffectofcodeliveryofanticancerdrugsintoastrocytesisolatedfromhumanglioblastomamultiforme AT mansourehhashemi synergisticeffectofcodeliveryofanticancerdrugsintoastrocytesisolatedfromhumanglioblastomamultiforme AT alirezazali synergisticeffectofcodeliveryofanticancerdrugsintoastrocytesisolatedfromhumanglioblastomamultiforme
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The Synergistic Effect of Co-delivery of Anticancer Drugs Into Astrocytes Isolated From Human Glioblastoma Multiforme

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