Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells

Li Wan,1,* Xinglei Yao,1–3,* Francesco Faiola,3 Bojun Liu,4 Tianyuan Zhang,2 Yasuhiko Tabata,5 Hiroyuki Mizuguchi,6 Shinsaku Nakagawa,7 Jian-Qing Gao,2 Robert Chunhua Zhao1 1Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Med...

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Autores principales:	Wan L, Yao X, Faiola F, Liu B, Zhang T, Tabata Y, Mizuguchi H, Nakagawa S, Gao JQ, Zhao RC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Mesenchymal stem cells Adenovirus vectors Spermine-Pullulan Polymer Gene transduction Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a70645c3ae4409cac1c88baec1f3fd8
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spelling	oai:doaj.org-article:0a70645c3ae4409cac1c88baec1f3fd82021-12-02T07:22:51ZCoating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells1178-2013https://doaj.org/article/0a70645c3ae4409cac1c88baec1f3fd82016-12-01T00:00:00Zhttps://www.dovepress.com/coating-with-spermine-pullulan-polymer-enhances-adenoviral-transductio-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Li Wan,1,* Xinglei Yao,1–3,* Francesco Faiola,3 Bojun Liu,4 Tianyuan Zhang,2 Yasuhiko Tabata,5 Hiroyuki Mizuguchi,6 Shinsaku Nakagawa,7 Jian-Qing Gao,2 Robert Chunhua Zhao1 1Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing, 2Institute of Pharmaceutics, Zhejiang University, Hangzhou, 3State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 4YouAn Hospital, Capital Medical University, Beijing, People’s Republic of China; 5Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 6Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, 7Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan *These authors contributed equally to this work Abstract: Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression. To overcome this problem, we developed an Adv coated with a spermine-pullulan (SP) cationic polymer and investigated its physicochemical properties and internalization mechanisms. We demonstrated that the SP coating could enhance adenoviral transduction of MSCs without detectable cytotoxicity or effects on differentiation. Our results argue in favor of the potentiality of the SP-coated Adv as a prototype vector for efficient and safe transduction of MSCs. Keywords: mesenchymal stem cells, adenovirus vectors, spermine-pullulan, polymer, gene transductionWan LYao XFaiola FLiu BZhang TTabata YMizuguchi HNakagawa SGao JQZhao RCDove Medical PressarticleMesenchymal stem cellsAdenovirus vectorsSpermine-PullulanPolymerGene transductionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6763-6769 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mesenchymal stem cells Adenovirus vectors Spermine-Pullulan Polymer Gene transduction Medicine (General) R5-920
spellingShingle	Mesenchymal stem cells Adenovirus vectors Spermine-Pullulan Polymer Gene transduction Medicine (General) R5-920 Wan L Yao X Faiola F Liu B Zhang T Tabata Y Mizuguchi H Nakagawa S Gao JQ Zhao RC Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
description	Li Wan,1,* Xinglei Yao,1–3,* Francesco Faiola,3 Bojun Liu,4 Tianyuan Zhang,2 Yasuhiko Tabata,5 Hiroyuki Mizuguchi,6 Shinsaku Nakagawa,7 Jian-Qing Gao,2 Robert Chunhua Zhao1 1Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing, 2Institute of Pharmaceutics, Zhejiang University, Hangzhou, 3State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 4YouAn Hospital, Capital Medical University, Beijing, People’s Republic of China; 5Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 6Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, 7Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan *These authors contributed equally to this work Abstract: Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression. To overcome this problem, we developed an Adv coated with a spermine-pullulan (SP) cationic polymer and investigated its physicochemical properties and internalization mechanisms. We demonstrated that the SP coating could enhance adenoviral transduction of MSCs without detectable cytotoxicity or effects on differentiation. Our results argue in favor of the potentiality of the SP-coated Adv as a prototype vector for efficient and safe transduction of MSCs. Keywords: mesenchymal stem cells, adenovirus vectors, spermine-pullulan, polymer, gene transduction
format	article
author	Wan L Yao X Faiola F Liu B Zhang T Tabata Y Mizuguchi H Nakagawa S Gao JQ Zhao RC
author_facet	Wan L Yao X Faiola F Liu B Zhang T Tabata Y Mizuguchi H Nakagawa S Gao JQ Zhao RC
author_sort	Wan L
title	Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
title_short	Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
title_full	Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
title_fullStr	Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
title_full_unstemmed	Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
title_sort	coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/0a70645c3ae4409cac1c88baec1f3fd8
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Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells

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