The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>

Many aspects regarding the role of lipopeptides (LPs) in bacterial interaction with plants are not clear yet. Of particular interest is the LP family of surfactin, immunogenic molecules involved in induced systemic resistance (ISR) and the bacterial colonization of plant surfaces. We hypothesize tha...

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Autores principales:	Alexandra Stoll, Ricardo Salvatierra-Martínez, Máximo González, Michael Araya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	colonization pattern population density surfactin production ISR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0a75762321eb4e31bd8909e40b786023
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spelling	oai:doaj.org-article:0a75762321eb4e31bd8909e40b7860232021-11-25T18:24:35ZThe Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>10.3390/microorganisms91122512076-2607https://doaj.org/article/0a75762321eb4e31bd8909e40b7860232021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2251https://doaj.org/toc/2076-2607Many aspects regarding the role of lipopeptides (LPs) in bacterial interaction with plants are not clear yet. Of particular interest is the LP family of surfactin, immunogenic molecules involved in induced systemic resistance (ISR) and the bacterial colonization of plant surfaces. We hypothesize that the concentration of surfactin produced by a strain correlates directly with its ability to colonize and persist on different plant surfaces, which conditions its capacity to trigger ISR. We used two <i>Bacillus velezensis strains</i> (BBC023 and BBC047), whose antagonistic potential in vitro is practically identical, but not on plant surfaces. The surfactin production of BBC047 is 1/3 higher than that of BBC023. Population density and SEM images revealed stable biofilms of BBC047 on leaves and roots, activating ISR on both plant surfaces. Despite its lower surfactin production, strain BBC023 assembled stable biofilms on roots and activated ISR. However, on leaves only isolated, unstructured populations were observed, which could not activate ISR. Thus, the ability of a strain to effectively colonize a plant surface is not only determined through its production of surfactin. Multiple aspects, such as environmental stressors or compensation mechanisms may influence the process. Finally, the importance of surfactin lies in its impacts on biofilm formation and stable colonization, which finally enables its activity as an elicitor of ISR.Alexandra StollRicardo Salvatierra-MartínezMáximo GonzálezMichael ArayaMDPI AGarticlecolonization patternpopulation densitysurfactin productionISRBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2251, p 2251 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colonization pattern population density surfactin production ISR Biology (General) QH301-705.5
spellingShingle	colonization pattern population density surfactin production ISR Biology (General) QH301-705.5 Alexandra Stoll Ricardo Salvatierra-Martínez Máximo González Michael Araya The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
description	Many aspects regarding the role of lipopeptides (LPs) in bacterial interaction with plants are not clear yet. Of particular interest is the LP family of surfactin, immunogenic molecules involved in induced systemic resistance (ISR) and the bacterial colonization of plant surfaces. We hypothesize that the concentration of surfactin produced by a strain correlates directly with its ability to colonize and persist on different plant surfaces, which conditions its capacity to trigger ISR. We used two <i>Bacillus velezensis strains</i> (BBC023 and BBC047), whose antagonistic potential in vitro is practically identical, but not on plant surfaces. The surfactin production of BBC047 is 1/3 higher than that of BBC023. Population density and SEM images revealed stable biofilms of BBC047 on leaves and roots, activating ISR on both plant surfaces. Despite its lower surfactin production, strain BBC023 assembled stable biofilms on roots and activated ISR. However, on leaves only isolated, unstructured populations were observed, which could not activate ISR. Thus, the ability of a strain to effectively colonize a plant surface is not only determined through its production of surfactin. Multiple aspects, such as environmental stressors or compensation mechanisms may influence the process. Finally, the importance of surfactin lies in its impacts on biofilm formation and stable colonization, which finally enables its activity as an elicitor of ISR.
format	article
author	Alexandra Stoll Ricardo Salvatierra-Martínez Máximo González Michael Araya
author_facet	Alexandra Stoll Ricardo Salvatierra-Martínez Máximo González Michael Araya
author_sort	Alexandra Stoll
title	The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
title_short	The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
title_full	The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
title_fullStr	The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
title_full_unstemmed	The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>
title_sort	role of surfactin production by <i>bacillus velezensis</i> on colonization, biofilm formation on tomato root and leaf surfaces and subsequent protection (isr) against <i>botrytis cinerea</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0a75762321eb4e31bd8909e40b786023
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The Role of Surfactin Production by <i>Bacillus velezensis</i> on Colonization, Biofilm Formation on Tomato Root and Leaf Surfaces and Subsequent Protection (ISR) against <i>Botrytis cinerea</i>

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