Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

Abstract Three-dimensional (3D) configuration of in vitro cultivated cells has been recognised as a valuable tool in developing stem cell and cancer cell therapy. However, currently available imaging approaches for live cells have drawbacks, including unsatisfactory resolution, lack of cross-section...

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Autores principales:	Chia-Ying Tsai, Cheng-Hung Shih, Hsiao-Sang Chu, Yi-Ting Hsieh, Sheng-Lung Huang, Wei-Li Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a76503ee2374639852e6f6adcf55f4d
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spelling	oai:doaj.org-article:0a76503ee2374639852e6f6adcf55f4d2021-12-02T14:11:28ZSubmicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems10.1038/s41598-021-82178-42045-2322https://doaj.org/article/0a76503ee2374639852e6f6adcf55f4d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82178-4https://doaj.org/toc/2045-2322Abstract Three-dimensional (3D) configuration of in vitro cultivated cells has been recognised as a valuable tool in developing stem cell and cancer cell therapy. However, currently available imaging approaches for live cells have drawbacks, including unsatisfactory resolution, lack of cross-sectional and 3D images, and poor penetration of multi-layered cell products, especially when cells are cultivated on semitransparent carriers. Herein, we report a prototype of a full-field optical coherence tomography (FF-OCT) system with isotropic submicron spatial resolution in en face and cross-sectional views that provides a label-free, non-invasive platform with high-resolution 3D imaging. We validated the imaging power of this prototype by examining (1) cultivated neuron cells (N2A cell line); (2) multilayered, cultivated limbal epithelial sheets (mCLESs); (3) neuron cells (N2A cell line) and mCLESs cultivated on a semitransparent amniotic membrane (stAM); and (4) directly adherent colonies of neuron-like cells (DACNs) covered by limbal epithelial cell sheets. Our FF-OCT exhibited a penetrance of up to 150 μm in a multilayered cell sheet and displayed the morphological differences of neurons and epithelial cells in complex coculture systems. This FF-OCT is expected to facilitate the visualisation of cultivated cell products in vitro and has a high potential for cell therapy and translational medicine research.Chia-Ying TsaiCheng-Hung ShihHsiao-Sang ChuYi-Ting HsiehSheng-Lung HuangWei-Li ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-Ying Tsai Cheng-Hung Shih Hsiao-Sang Chu Yi-Ting Hsieh Sheng-Lung Huang Wei-Li Chen Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
description	Abstract Three-dimensional (3D) configuration of in vitro cultivated cells has been recognised as a valuable tool in developing stem cell and cancer cell therapy. However, currently available imaging approaches for live cells have drawbacks, including unsatisfactory resolution, lack of cross-sectional and 3D images, and poor penetration of multi-layered cell products, especially when cells are cultivated on semitransparent carriers. Herein, we report a prototype of a full-field optical coherence tomography (FF-OCT) system with isotropic submicron spatial resolution in en face and cross-sectional views that provides a label-free, non-invasive platform with high-resolution 3D imaging. We validated the imaging power of this prototype by examining (1) cultivated neuron cells (N2A cell line); (2) multilayered, cultivated limbal epithelial sheets (mCLESs); (3) neuron cells (N2A cell line) and mCLESs cultivated on a semitransparent amniotic membrane (stAM); and (4) directly adherent colonies of neuron-like cells (DACNs) covered by limbal epithelial cell sheets. Our FF-OCT exhibited a penetrance of up to 150 μm in a multilayered cell sheet and displayed the morphological differences of neurons and epithelial cells in complex coculture systems. This FF-OCT is expected to facilitate the visualisation of cultivated cell products in vitro and has a high potential for cell therapy and translational medicine research.
format	article
author	Chia-Ying Tsai Cheng-Hung Shih Hsiao-Sang Chu Yi-Ting Hsieh Sheng-Lung Huang Wei-Li Chen
author_facet	Chia-Ying Tsai Cheng-Hung Shih Hsiao-Sang Chu Yi-Ting Hsieh Sheng-Lung Huang Wei-Li Chen
author_sort	Chia-Ying Tsai
title	Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
title_short	Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
title_full	Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
title_fullStr	Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
title_full_unstemmed	Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems
title_sort	submicron spatial resolution optical coherence tomography for visualising the 3d structures of cells cultivated in complex culture systems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0a76503ee2374639852e6f6adcf55f4d
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Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

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