Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis

ABSTRACT: Neuropeptide S (NPS) and its receptor neuropeptide S receptor 1 (NPSR1) have been suggested to regulate many physiological processes in the central nervous system (CNS), such as arousal, anxiety, and food intake in mammals and birds, however, the functionality and tissue expression of this...

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Autores principales:	Chao Fang, Jiannan Zhang, Yiping Wan, Zejiao Li, Feiyang Qi, Yuanhao Dang, Juan Li, Yajun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	chicken NPS NPSR1 tissue distribution functional analysis Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/0a7c83b2b2e34eb9a55188ed4553c851
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spelling	oai:doaj.org-article:0a7c83b2b2e34eb9a55188ed4553c8512021-11-24T04:21:44ZNeuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis0032-579110.1016/j.psj.2021.101445https://doaj.org/article/0a7c83b2b2e34eb9a55188ed4553c8512021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121004685https://doaj.org/toc/0032-5791ABSTRACT: Neuropeptide S (NPS) and its receptor neuropeptide S receptor 1 (NPSR1) have been suggested to regulate many physiological processes in the central nervous system (CNS), such as arousal, anxiety, and food intake in mammals and birds, however, the functionality and tissue expression of this NPS-NPSR1 system remain unknown in birds. Here, we cloned NPS and NPSR1 cDNAs from the chicken brain and reported their functionality and tissue expression. The cloned chicken NPS is predicted to encode a mature NPS peptide of 20 amino acids, which shows a remarkable sequence identity (∼94%) among tetrapod species examined, while NPSR1 encodes a receptor of 373 amino acids conserved across vertebrates. Using cell-based luciferase reporter systems, we demonstrated that chicken NPS could potently activate NPSR1 expressed in vitro and thus stimulates multiple signaling pathways, including calcium mobilization, cyclic adenosine monophosphate/protein kinase A (cAMP/PKA), and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathways, indicating that NPS actions could be mediated by NPSR1 in birds. Quantitative real-time PCR revealed that NPS and NPSR1 are widely expressed in chicken tissues, including the hypothalamus, and NPSR1 expression is likely controlled by a promoter upstream exon 1, which shows strong promoter activities in cultured DF-1 cells. Taken together, our data provide the first proof that the avian NPS-NPSR1 system is functional and helps to explore the conserved role of NPS and NPSR1 signaling in tetrapods.Chao FangJiannan ZhangYiping WanZejiao LiFeiyang QiYuanhao DangJuan LiYajun WangElsevierarticlechickenNPSNPSR1tissue distributionfunctional analysisAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101445- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken NPS NPSR1 tissue distribution functional analysis Animal culture SF1-1100
spellingShingle	chicken NPS NPSR1 tissue distribution functional analysis Animal culture SF1-1100 Chao Fang Jiannan Zhang Yiping Wan Zejiao Li Feiyang Qi Yuanhao Dang Juan Li Yajun Wang Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
description	ABSTRACT: Neuropeptide S (NPS) and its receptor neuropeptide S receptor 1 (NPSR1) have been suggested to regulate many physiological processes in the central nervous system (CNS), such as arousal, anxiety, and food intake in mammals and birds, however, the functionality and tissue expression of this NPS-NPSR1 system remain unknown in birds. Here, we cloned NPS and NPSR1 cDNAs from the chicken brain and reported their functionality and tissue expression. The cloned chicken NPS is predicted to encode a mature NPS peptide of 20 amino acids, which shows a remarkable sequence identity (∼94%) among tetrapod species examined, while NPSR1 encodes a receptor of 373 amino acids conserved across vertebrates. Using cell-based luciferase reporter systems, we demonstrated that chicken NPS could potently activate NPSR1 expressed in vitro and thus stimulates multiple signaling pathways, including calcium mobilization, cyclic adenosine monophosphate/protein kinase A (cAMP/PKA), and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathways, indicating that NPS actions could be mediated by NPSR1 in birds. Quantitative real-time PCR revealed that NPS and NPSR1 are widely expressed in chicken tissues, including the hypothalamus, and NPSR1 expression is likely controlled by a promoter upstream exon 1, which shows strong promoter activities in cultured DF-1 cells. Taken together, our data provide the first proof that the avian NPS-NPSR1 system is functional and helps to explore the conserved role of NPS and NPSR1 signaling in tetrapods.
format	article
author	Chao Fang Jiannan Zhang Yiping Wan Zejiao Li Feiyang Qi Yuanhao Dang Juan Li Yajun Wang
author_facet	Chao Fang Jiannan Zhang Yiping Wan Zejiao Li Feiyang Qi Yuanhao Dang Juan Li Yajun Wang
author_sort	Chao Fang
title	Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
title_short	Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
title_full	Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
title_fullStr	Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
title_full_unstemmed	Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis
title_sort	neuropeptide s (nps) and its receptor (npsr1) in chickens: cloning, tissue expression, and functional analysis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0a7c83b2b2e34eb9a55188ed4553c851
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Neuropeptide S (NPS) and its receptor (NPSR1) in chickens: cloning, tissue expression, and functional analysis

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