Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019

ABSTRACT: Mycoplasma synoviae (M. synoviae) has been identified worldwide to cause respiratory diseases, infectious synovitis, airsacculitis, and eggshell apex abnormalities (EAA) in commercial chickens, which results in substantial economic losses to the poultry industry. Therefore, in this study,...

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Autores principales:	Chaoge Sui, Hao Cui, Jun Ji, Xin Xu, Yunchao Kan, Lunguang Yao, Yingzuo Bi, Xinheng Zhang, Qingmei Xie
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	epidemiological investigation, Mycoplasma synoviae vlhA genotyping phylogenetic analysis Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/0a7eab223f3b4df399209f56ea5730b8
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spelling	oai:doaj.org-article:0a7eab223f3b4df399209f56ea5730b82021-11-24T04:22:57ZEpidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 20190032-579110.1016/j.psj.2021.101522https://doaj.org/article/0a7eab223f3b4df399209f56ea5730b82022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005447https://doaj.org/toc/0032-5791ABSTRACT: Mycoplasma synoviae (M. synoviae) has been identified worldwide to cause respiratory diseases, infectious synovitis, airsacculitis, and eggshell apex abnormalities (EAA) in commercial chickens, which results in substantial economic losses to the poultry industry. Therefore, in this study, 258 flocks were investigated between 2017 and 2019 for M. synoviae by screening samples from Central China. Subsequently, 129 M. synoviae strains were isolated, with a positive rate of 50%. Moreover, a higher incidence of M. Synoviae infections was in layers (74.1%) than in broilers (20%) in this study. The 5′-end conserved segment of the variable lipoprotein hemagglutinin A (vlhA) gene of these isolates was then cloned and sequenced because it is a common genomic target identified so far for M. synoviae genotyping. Genotyping of all isolates was based on the phylogenetic analysis and length analysis of the proline-rich-repeat (PRR) regions, respectively. Phylogenetic analysis based on 5′-end conserved segment of the vlhA gene (76–421 nt) assigned the majority of the occurring strains as being from group 6, and others from groups 2 and 3. Results identified that these isolates were of 6 types: A (38aa), D (23aa), E (19aa), I (28aa), J (20aa), and L (35aa), based on the size of the PRR region analysis. Furthermore, most of the isolates (81.4% were identified as type L. Additionally, the epidemic types included only I and L in 2017; however, the types rose to 5 (A, D, E, I, L) in 2018 and rose to 6 (A, D, E, I, J, L) in 2019. These data showed the genotype diversity of M. synoviae in Central China. The high rate of positive flocks suggests the urgent need to take real-time supervisory controls of this Mycoplasma species in avian flocks.Chaoge SuiHao CuiJun JiXin XuYunchao KanLunguang YaoYingzuo BiXinheng ZhangQingmei XieElsevierarticleepidemiological investigation, Mycoplasma synoviaevlhAgenotypingphylogenetic analysisAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101522- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	epidemiological investigation, Mycoplasma synoviae vlhA genotyping phylogenetic analysis Animal culture SF1-1100
spellingShingle	epidemiological investigation, Mycoplasma synoviae vlhA genotyping phylogenetic analysis Animal culture SF1-1100 Chaoge Sui Hao Cui Jun Ji Xin Xu Yunchao Kan Lunguang Yao Yingzuo Bi Xinheng Zhang Qingmei Xie Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
description	ABSTRACT: Mycoplasma synoviae (M. synoviae) has been identified worldwide to cause respiratory diseases, infectious synovitis, airsacculitis, and eggshell apex abnormalities (EAA) in commercial chickens, which results in substantial economic losses to the poultry industry. Therefore, in this study, 258 flocks were investigated between 2017 and 2019 for M. synoviae by screening samples from Central China. Subsequently, 129 M. synoviae strains were isolated, with a positive rate of 50%. Moreover, a higher incidence of M. Synoviae infections was in layers (74.1%) than in broilers (20%) in this study. The 5′-end conserved segment of the variable lipoprotein hemagglutinin A (vlhA) gene of these isolates was then cloned and sequenced because it is a common genomic target identified so far for M. synoviae genotyping. Genotyping of all isolates was based on the phylogenetic analysis and length analysis of the proline-rich-repeat (PRR) regions, respectively. Phylogenetic analysis based on 5′-end conserved segment of the vlhA gene (76–421 nt) assigned the majority of the occurring strains as being from group 6, and others from groups 2 and 3. Results identified that these isolates were of 6 types: A (38aa), D (23aa), E (19aa), I (28aa), J (20aa), and L (35aa), based on the size of the PRR region analysis. Furthermore, most of the isolates (81.4% were identified as type L. Additionally, the epidemic types included only I and L in 2017; however, the types rose to 5 (A, D, E, I, L) in 2018 and rose to 6 (A, D, E, I, J, L) in 2019. These data showed the genotype diversity of M. synoviae in Central China. The high rate of positive flocks suggests the urgent need to take real-time supervisory controls of this Mycoplasma species in avian flocks.
format	article
author	Chaoge Sui Hao Cui Jun Ji Xin Xu Yunchao Kan Lunguang Yao Yingzuo Bi Xinheng Zhang Qingmei Xie
author_facet	Chaoge Sui Hao Cui Jun Ji Xin Xu Yunchao Kan Lunguang Yao Yingzuo Bi Xinheng Zhang Qingmei Xie
author_sort	Chaoge Sui
title	Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
title_short	Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
title_full	Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
title_fullStr	Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
title_full_unstemmed	Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019
title_sort	epidemiological investigations and locally determined genotype diversity of mycoplasma synoviae in central china from 2017 to 2019
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/0a7eab223f3b4df399209f56ea5730b8
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_version_	1718416017636458496

Epidemiological investigations and locally determined genotype diversity of Mycoplasma synoviae in Central China from 2017 to 2019

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