Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents

Absorption through the skin of topically applied chemicals is relevant for both formulation development and safety assessment, especially in the early stages of development. However, the supply of human skin is limited, and the traditional in vitro methods are of low throughput. As an alternative, a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bálint Sinkó, Vivien Bárdos, Dániel Vesztergombi, Szabina Kádár, Petra Malcsiner, Anne Moustie, Chantal Jouy, Krisztina Takács-Novák, Sebastien Grégoire
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Skin-PAMPA formulation skin barrier permeability pig skin safety testing Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/0a7f65e9216e43309bd3d7f6dabc0762
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a7f65e9216e43309bd3d7f6dabc0762
record_format	dspace
spelling	oai:doaj.org-article:0a7f65e9216e43309bd3d7f6dabc07622021-11-25T18:40:26ZUse of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents10.3390/pharmaceutics131117581999-4923https://doaj.org/article/0a7f65e9216e43309bd3d7f6dabc07622021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1758https://doaj.org/toc/1999-4923Absorption through the skin of topically applied chemicals is relevant for both formulation development and safety assessment, especially in the early stages of development. However, the supply of human skin is limited, and the traditional in vitro methods are of low throughput. As an alternative, an artificial membrane-based Skin Parallel Artificial Membrane Permeability Assay (Skin-PAMPA) has been developed to mimic the permeability through the stratum corneum. In this study, this assay was used to measure the permeability of a model compound, 4-phenylethyl-resorcinol (PER), dissolved in 13 different solvents that are commonly used in cosmetic formulation development. The study was performed at concentrations close to the saturated solution of PER in each solvent to investigate the maximum thermodynamic potential of the solvents. The permeability of PER in selected solvents was also measured on ex vivo pig skin for comparison. Pig ear skin is an accepted alternative model of human skin. The permeability coefficient, which is independent of the concentration of the applied solution, showed a good correlation (<i>R<sup>2</sup></i> = 0.844) between the Skin-PAMPA and the pig skin permeation data. Our results support the use of the Skin-PAMPA to screen the suitability of different solvents for non-polar compounds at an early stage of formulation development.Bálint SinkóVivien BárdosDániel VesztergombiSzabina KádárPetra MalcsinerAnne MoustieChantal JouyKrisztina Takács-NovákSebastien GrégoireMDPI AGarticleSkin-PAMPAformulationskin barrierpermeabilitypig skinsafety testingPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1758, p 1758 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Skin-PAMPA formulation skin barrier permeability pig skin safety testing Pharmacy and materia medica RS1-441
spellingShingle	Skin-PAMPA formulation skin barrier permeability pig skin safety testing Pharmacy and materia medica RS1-441 Bálint Sinkó Vivien Bárdos Dániel Vesztergombi Szabina Kádár Petra Malcsiner Anne Moustie Chantal Jouy Krisztina Takács-Novák Sebastien Grégoire Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
description	Absorption through the skin of topically applied chemicals is relevant for both formulation development and safety assessment, especially in the early stages of development. However, the supply of human skin is limited, and the traditional in vitro methods are of low throughput. As an alternative, an artificial membrane-based Skin Parallel Artificial Membrane Permeability Assay (Skin-PAMPA) has been developed to mimic the permeability through the stratum corneum. In this study, this assay was used to measure the permeability of a model compound, 4-phenylethyl-resorcinol (PER), dissolved in 13 different solvents that are commonly used in cosmetic formulation development. The study was performed at concentrations close to the saturated solution of PER in each solvent to investigate the maximum thermodynamic potential of the solvents. The permeability of PER in selected solvents was also measured on ex vivo pig skin for comparison. Pig ear skin is an accepted alternative model of human skin. The permeability coefficient, which is independent of the concentration of the applied solution, showed a good correlation (<i>R<sup>2</sup></i> = 0.844) between the Skin-PAMPA and the pig skin permeation data. Our results support the use of the Skin-PAMPA to screen the suitability of different solvents for non-polar compounds at an early stage of formulation development.
format	article
author	Bálint Sinkó Vivien Bárdos Dániel Vesztergombi Szabina Kádár Petra Malcsiner Anne Moustie Chantal Jouy Krisztina Takács-Novák Sebastien Grégoire
author_facet	Bálint Sinkó Vivien Bárdos Dániel Vesztergombi Szabina Kádár Petra Malcsiner Anne Moustie Chantal Jouy Krisztina Takács-Novák Sebastien Grégoire
author_sort	Bálint Sinkó
title	Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
title_short	Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
title_full	Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
title_fullStr	Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
title_full_unstemmed	Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents
title_sort	use of an in vitro skin parallel artificial membrane assay (skin-pampa) as a screening tool to compare transdermal permeability of model compound 4-phenylethyl-resorcinol dissolved in different solvents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0a7f65e9216e43309bd3d7f6dabc0762
work_keys_str_mv	AT balintsinko useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT vivienbardos useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT danielvesztergombi useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT szabinakadar useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT petramalcsiner useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT annemoustie useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT chantaljouy useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT krisztinatakacsnovak useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents AT sebastiengregoire useofaninvitroskinparallelartificialmembraneassayskinpampaasascreeningtooltocomparetransdermalpermeabilityofmodelcompound4phenylethylresorcinoldissolvedindifferentsolvents
_version_	1718410814135730176

Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents

Ejemplares similares