The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus

Abstract Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Asuka Hirooka, Mayuko Hamada, Daiki Fujiyama, Keiko Takanami, Yasuhisa Kobayashi, Takumi Oti, Yukitoshi Katayama, Tatsuya Sakamoto, Hirotaka Sakamoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a8249bc7c864babbc775dbdb389e375
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a8249bc7c864babbc775dbdb389e375
record_format	dspace
spelling	oai:doaj.org-article:0a8249bc7c864babbc775dbdb389e3752021-12-02T17:14:29ZThe gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus10.1038/s41598-021-92528-x2045-2322https://doaj.org/article/0a8249bc7c864babbc775dbdb389e3752021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92528-xhttps://doaj.org/toc/2045-2322Abstract Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be paid to the evolutionary relationship of GRP/bombesin and their receptors in vertebrates. We have classified the peptides and their receptors from the phylogenetic viewpoint using a newly established genetic database and bioinformatics. Here we show, by using a clawed frog (Xenopus tropicalis), that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frog species. To understand the derivation of GRP system in the ancestor of mammals, we have focused on the GRP system in Xenopus. Gene expression analyses combined with immunohistochemistry and Western blotting experiments demonstrated that GRP peptides and their receptors are distributed in the brain and stomach of Xenopus. We conclude that GRP peptides and their receptors have evolved from ancestral (GRP-like peptide) homologues to play multiple roles in both the gut and the brain as one of the ‘gut-brain peptide’ systems.Asuka HirookaMayuko HamadaDaiki FujiyamaKeiko TakanamiYasuhisa KobayashiTakumi OtiYukitoshi KatayamaTatsuya SakamotoHirotaka SakamotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Asuka Hirooka Mayuko Hamada Daiki Fujiyama Keiko Takanami Yasuhisa Kobayashi Takumi Oti Yukitoshi Katayama Tatsuya Sakamoto Hirotaka Sakamoto The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
description	Abstract Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be paid to the evolutionary relationship of GRP/bombesin and their receptors in vertebrates. We have classified the peptides and their receptors from the phylogenetic viewpoint using a newly established genetic database and bioinformatics. Here we show, by using a clawed frog (Xenopus tropicalis), that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frog species. To understand the derivation of GRP system in the ancestor of mammals, we have focused on the GRP system in Xenopus. Gene expression analyses combined with immunohistochemistry and Western blotting experiments demonstrated that GRP peptides and their receptors are distributed in the brain and stomach of Xenopus. We conclude that GRP peptides and their receptors have evolved from ancestral (GRP-like peptide) homologues to play multiple roles in both the gut and the brain as one of the ‘gut-brain peptide’ systems.
format	article
author	Asuka Hirooka Mayuko Hamada Daiki Fujiyama Keiko Takanami Yasuhisa Kobayashi Takumi Oti Yukitoshi Katayama Tatsuya Sakamoto Hirotaka Sakamoto
author_facet	Asuka Hirooka Mayuko Hamada Daiki Fujiyama Keiko Takanami Yasuhisa Kobayashi Takumi Oti Yukitoshi Katayama Tatsuya Sakamoto Hirotaka Sakamoto
author_sort	Asuka Hirooka
title	The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
title_short	The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
title_full	The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
title_fullStr	The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
title_full_unstemmed	The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus
title_sort	gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering xenopus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0a8249bc7c864babbc775dbdb389e375
work_keys_str_mv	AT asukahirooka thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT mayukohamada thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT daikifujiyama thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT keikotakanami thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT yasuhisakobayashi thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT takumioti thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT yukitoshikatayama thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT tatsuyasakamoto thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT hirotakasakamoto thegastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT asukahirooka gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT mayukohamada gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT daikifujiyama gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT keikotakanami gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT yasuhisakobayashi gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT takumioti gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT yukitoshikatayama gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT tatsuyasakamoto gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus AT hirotakasakamoto gastrinreleasingpeptidebombesinsystemrevisitedbyareverseevolutionarystudyconsideringxenopus
_version_	1718381302891151360

The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus

Ejemplares similares