A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae

Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in mycoplasma pneumoniae in China. Rapid and accurate identification of mycoplasma pneumonia<...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anyan Wang, Zhenhua Wu, Yuhang Huang, Hongbo Zhou, Lei Wu, Chunping Jia, Qiang Chen, Jianlong Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mycoplasma pneumoniae</i> macrolides resistance mutations microfluidic 3D-printed sample-to-answer Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/0a87d5369b914d6ebaa4981a8402d61e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0a87d5369b914d6ebaa4981a8402d61e
record_format	dspace
spelling	oai:doaj.org-article:0a87d5369b914d6ebaa4981a8402d61e2021-11-25T16:55:08ZA 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i> 10.3390/bios111104272079-6374https://doaj.org/article/0a87d5369b914d6ebaa4981a8402d61e2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/427https://doaj.org/toc/2079-6374Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in <i>mycoplasma pneumonia</i><i>e</i> in China. Rapid and accurate identification of <i>mycoplasma pneumonia</i><i>e</i> and its mutant type is necessary for precise medication. This paper presents a 3D-printed microfluidic device to achieve this. By 3D printing, the stereoscopic structures such as microvalves, reservoirs, drainage tubes, and connectors were fabricated in one step. The device integrated commercial polymerase chain reaction (PCR) tubes as PCR chambers. The detection was a sample-to-answer procedure. First, the sample, a PCR mix, and mineral oil were respectively added to the reservoirs on the device. Next, the device automatically mixed the sample with the PCR mix and evenly dispensed the mixed solution and mineral oil into the PCR chambers, which were preloaded with the specified primers and probes. Subsequently, quantitative real-time PCR (qPCR) was carried out with the homemade instrument. Within 80 min, <i>mycoplasma pneumonia</i><i>e</i> and its mutation type in the clinical samples were determined, which was verified by DNA sequencing. The easy-to-make and easy-to-use device provides a rapid and integrated detection approach for pathogens and antibiotic resistance mutations, which is urgently needed on the infection scene and in hospital emergency departments.Anyan WangZhenhua WuYuhang HuangHongbo ZhouLei WuChunping JiaQiang ChenJianlong ZhaoMDPI AGarticle<i>Mycoplasma pneumoniae</i>macrolidesresistance mutationsmicrofluidic3D-printedsample-to-answerBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 427, p 427 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mycoplasma pneumoniae</i> macrolides resistance mutations microfluidic 3D-printed sample-to-answer Biotechnology TP248.13-248.65
spellingShingle	<i>Mycoplasma pneumoniae</i> macrolides resistance mutations microfluidic 3D-printed sample-to-answer Biotechnology TP248.13-248.65 Anyan Wang Zhenhua Wu Yuhang Huang Hongbo Zhou Lei Wu Chunping Jia Qiang Chen Jianlong Zhao A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
description	Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in <i>mycoplasma pneumonia</i><i>e</i> in China. Rapid and accurate identification of <i>mycoplasma pneumonia</i><i>e</i> and its mutant type is necessary for precise medication. This paper presents a 3D-printed microfluidic device to achieve this. By 3D printing, the stereoscopic structures such as microvalves, reservoirs, drainage tubes, and connectors were fabricated in one step. The device integrated commercial polymerase chain reaction (PCR) tubes as PCR chambers. The detection was a sample-to-answer procedure. First, the sample, a PCR mix, and mineral oil were respectively added to the reservoirs on the device. Next, the device automatically mixed the sample with the PCR mix and evenly dispensed the mixed solution and mineral oil into the PCR chambers, which were preloaded with the specified primers and probes. Subsequently, quantitative real-time PCR (qPCR) was carried out with the homemade instrument. Within 80 min, <i>mycoplasma pneumonia</i><i>e</i> and its mutation type in the clinical samples were determined, which was verified by DNA sequencing. The easy-to-make and easy-to-use device provides a rapid and integrated detection approach for pathogens and antibiotic resistance mutations, which is urgently needed on the infection scene and in hospital emergency departments.
format	article
author	Anyan Wang Zhenhua Wu Yuhang Huang Hongbo Zhou Lei Wu Chunping Jia Qiang Chen Jianlong Zhao
author_facet	Anyan Wang Zhenhua Wu Yuhang Huang Hongbo Zhou Lei Wu Chunping Jia Qiang Chen Jianlong Zhao
author_sort	Anyan Wang
title	A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
title_short	A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
title_full	A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
title_fullStr	A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
title_full_unstemmed	A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>
title_sort	3d-printed microfluidic device for qpcr detection of macrolide-resistant mutations of <i>mycoplasma pneumoniae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0a87d5369b914d6ebaa4981a8402d61e
work_keys_str_mv	AT anyanwang a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT zhenhuawu a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT yuhanghuang a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT hongbozhou a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT leiwu a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT chunpingjia a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT qiangchen a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT jianlongzhao a3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT anyanwang 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT zhenhuawu 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT yuhanghuang 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT hongbozhou 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT leiwu 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT chunpingjia 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT qiangchen 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei AT jianlongzhao 3dprintedmicrofluidicdeviceforqpcrdetectionofmacrolideresistantmutationsofimycoplasmapneumoniaei
_version_	1718412817746362368

A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of <i>Mycoplasma pneumoniae</i>

Ejemplares similares