Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.

<h4>Background</h4>Accurate diagnosis of tuberculosis (TB) due to infection with Mycobacterium bovis is notoriously difficult in live animals, yet important if we are to understand the epidemiology of TB and devise effective strategies to limit its spread. Currently available tests for d...

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Autores principales:	Julian A Drewe, Alexandra J Tomlinson, Neil J Walker, Richard J Delahay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a8aa72f34d643c2a75a8c5313e6b8ed
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spelling	oai:doaj.org-article:0a8aa72f34d643c2a75a8c5313e6b8ed2021-12-02T20:20:42ZDiagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.1932-620310.1371/journal.pone.0011196https://doaj.org/article/0a8aa72f34d643c2a75a8c5313e6b8ed2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585404/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Accurate diagnosis of tuberculosis (TB) due to infection with Mycobacterium bovis is notoriously difficult in live animals, yet important if we are to understand the epidemiology of TB and devise effective strategies to limit its spread. Currently available tests for diagnosing TB in live Eurasian badgers (Meles meles) remain unvalidated against a reliable gold standard. The aim of the present study was to evaluate the diagnostic accuracy and optimal use of three tests for TB in badgers in the absence of a gold standard.<h4>Methodology/principal findings</h4>A Bayesian approach was used to evaluate the diagnostic accuracy and optimal use of mycobacterial culture, gamma-interferon assay and a commercially available serological test using multiple samples collected from 305 live wild badgers. Although no single test was judged to be sufficiently sensitive and specific to be used as a sole diagnostic method, selective combined use of the three tests allowed guidelines to be formulated that allow a diagnosis to be made for individual animals with an estimated overall accuracy of 93% (range: 75% to 97%). Employing this approach in the study population of badgers resulted in approximately 13 out of 14 animals having their true infection status correctly classified from samples collected on a single capture.<h4>Conclusions/significance</h4>This method of interpretation represents a marked improvement on the current procedure for diagnosing M. bovis infection in live badgers. The results should be of use to inform future test and intervention strategies with the aim of reducing the incidence of TB in free-living wild badger populations.Julian A DreweAlexandra J TomlinsonNeil J WalkerRichard J DelahayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11196 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julian A Drewe Alexandra J Tomlinson Neil J Walker Richard J Delahay Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
description	<h4>Background</h4>Accurate diagnosis of tuberculosis (TB) due to infection with Mycobacterium bovis is notoriously difficult in live animals, yet important if we are to understand the epidemiology of TB and devise effective strategies to limit its spread. Currently available tests for diagnosing TB in live Eurasian badgers (Meles meles) remain unvalidated against a reliable gold standard. The aim of the present study was to evaluate the diagnostic accuracy and optimal use of three tests for TB in badgers in the absence of a gold standard.<h4>Methodology/principal findings</h4>A Bayesian approach was used to evaluate the diagnostic accuracy and optimal use of mycobacterial culture, gamma-interferon assay and a commercially available serological test using multiple samples collected from 305 live wild badgers. Although no single test was judged to be sufficiently sensitive and specific to be used as a sole diagnostic method, selective combined use of the three tests allowed guidelines to be formulated that allow a diagnosis to be made for individual animals with an estimated overall accuracy of 93% (range: 75% to 97%). Employing this approach in the study population of badgers resulted in approximately 13 out of 14 animals having their true infection status correctly classified from samples collected on a single capture.<h4>Conclusions/significance</h4>This method of interpretation represents a marked improvement on the current procedure for diagnosing M. bovis infection in live badgers. The results should be of use to inform future test and intervention strategies with the aim of reducing the incidence of TB in free-living wild badger populations.
format	article
author	Julian A Drewe Alexandra J Tomlinson Neil J Walker Richard J Delahay
author_facet	Julian A Drewe Alexandra J Tomlinson Neil J Walker Richard J Delahay
author_sort	Julian A Drewe
title	Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
title_short	Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
title_full	Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
title_fullStr	Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
title_full_unstemmed	Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
title_sort	diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0a8aa72f34d643c2a75a8c5313e6b8ed
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Diagnostic accuracy and optimal use of three tests for tuberculosis in live badgers.

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