Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation

Cuiping Yao,1,2,* Florian Rudnitzki,2,* Gereon Hüttmann,2,3 Zhenxi Zhang,1 Ramtin Rahmanzadeh2 1Key Laboratory of Biomedical Information Engineering of Education Ministry, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi&rsquo...

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Autores principales:	Yao CP, Rudnitzki F, Hüttmann G, Zhang ZX, Rahmanzadeh R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	cell membrane permeabilization optimization molecule delivery gold nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0a8bff5f1fdc496a9a8efff66e17db89
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spelling	oai:doaj.org-article:0a8bff5f1fdc496a9a8efff66e17db892021-12-02T00:54:30ZImportant factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation1178-2013https://doaj.org/article/0a8bff5f1fdc496a9a8efff66e17db892017-08-01T00:00:00Zhttps://www.dovepress.com/important-factors-for-cell-membrane-permeabilization-by-gold-nanoparti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cuiping Yao,1,2,* Florian Rudnitzki,2,* Gereon Hüttmann,2,3 Zhenxi Zhang,1 Ramtin Rahmanzadeh2 1Key Laboratory of Biomedical Information Engineering of Education Ministry, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, China; 2Institute of Biomedical Optics, University of Lübeck, Lübeck, 3Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Kiel, Germany *These authors contributed equally to this work Purpose: Pulsed-laser irradiation of light-absorbing gold nanoparticles (AuNPs) attached to cells transiently increases cell membrane permeability for targeted molecule delivery. Here, we targeted EGFR on the ovarian carcinoma cell line OVCAR-3 with AuNPs. In order to optimize membrane permeability and to demonstrate molecule delivery into adherent OVCAR-3 cells, we systematically investigated different experimental conditions. Materials and methods: AuNPs (30 nm) were functionalized by conjugation of the antibody cetuximab against EGFR. Selective binding of the particles was demonstrated by silver staining, multiphoton imaging, and fluorescence-lifetime imaging. After laser irradiation, membrane permeability of OVCAR-3 cells was studied under different conditions of AuNP concentration, cell-incubation medium, and cell–AuNP incubation time. Membrane permeability and cell viability were evaluated by flow cytometry, measuring propidium iodide and fluorescein isothiocyanate–dextran uptake. Results: Adherently growing OVCAR-3 cells can be effectively targeted with EGFR-AuNP. Laser irradiation led to successful permeabilization, and 150 kDa dextran was successfully delivered into cells with about 70% efficiency. Conclusion: Antibody-targeted and laser-irradiated AuNPs can be used to deliver molecules into adherent cells. Efficacy depends not only on laser parameters but also on AuNP:cell ratio, cell-incubation medium, and cell–AuNP incubation time. Keywords: cell-membrane permeabilization, optimization, molecule delivery, gold nanoparticlesYao CPRudnitzki FHüttmann GZhang ZXRahmanzadeh RDove Medical Pressarticlecell membrane permeabilizationoptimizationmolecule deliverygold nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5659-5672 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell membrane permeabilization optimization molecule delivery gold nanoparticles Medicine (General) R5-920
spellingShingle	cell membrane permeabilization optimization molecule delivery gold nanoparticles Medicine (General) R5-920 Yao CP Rudnitzki F Hüttmann G Zhang ZX Rahmanzadeh R Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
description	Cuiping Yao,1,2,* Florian Rudnitzki,2,* Gereon Hüttmann,2,3 Zhenxi Zhang,1 Ramtin Rahmanzadeh2 1Key Laboratory of Biomedical Information Engineering of Education Ministry, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, China; 2Institute of Biomedical Optics, University of Lübeck, Lübeck, 3Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Kiel, Germany *These authors contributed equally to this work Purpose: Pulsed-laser irradiation of light-absorbing gold nanoparticles (AuNPs) attached to cells transiently increases cell membrane permeability for targeted molecule delivery. Here, we targeted EGFR on the ovarian carcinoma cell line OVCAR-3 with AuNPs. In order to optimize membrane permeability and to demonstrate molecule delivery into adherent OVCAR-3 cells, we systematically investigated different experimental conditions. Materials and methods: AuNPs (30 nm) were functionalized by conjugation of the antibody cetuximab against EGFR. Selective binding of the particles was demonstrated by silver staining, multiphoton imaging, and fluorescence-lifetime imaging. After laser irradiation, membrane permeability of OVCAR-3 cells was studied under different conditions of AuNP concentration, cell-incubation medium, and cell–AuNP incubation time. Membrane permeability and cell viability were evaluated by flow cytometry, measuring propidium iodide and fluorescein isothiocyanate–dextran uptake. Results: Adherently growing OVCAR-3 cells can be effectively targeted with EGFR-AuNP. Laser irradiation led to successful permeabilization, and 150 kDa dextran was successfully delivered into cells with about 70% efficiency. Conclusion: Antibody-targeted and laser-irradiated AuNPs can be used to deliver molecules into adherent cells. Efficacy depends not only on laser parameters but also on AuNP:cell ratio, cell-incubation medium, and cell–AuNP incubation time. Keywords: cell-membrane permeabilization, optimization, molecule delivery, gold nanoparticles
format	article
author	Yao CP Rudnitzki F Hüttmann G Zhang ZX Rahmanzadeh R
author_facet	Yao CP Rudnitzki F Hüttmann G Zhang ZX Rahmanzadeh R
author_sort	Yao CP
title	Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
title_short	Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
title_full	Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
title_fullStr	Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
title_full_unstemmed	Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
title_sort	important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0a8bff5f1fdc496a9a8efff66e17db89
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Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation

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