Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> or 200 µg kg<sup>−1</sup> body weight to induce spermiation was compared in sterlet <i>Acipenser ruthenus</i>. All hormone treatme...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0a8f166be9be4144b232e6d1726ce1c6 |
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| Sumario: | Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> or 200 µg kg<sup>−1</sup> body weight to induce spermiation was compared in sterlet <i>Acipenser ruthenus</i>. All hormone treatments initially increased testosterone and 11-ketotestosterone, with a subsequent decline in testosterone but consistent high levels of 11-ketotestosterone at 48 and 72 h post-treatment. Spermiation did not differ between hormone-treated groups, and was not detected in controls receiving saline solution. Administration of the carp pituitary led to maximum sperm production 24 h post-treatment, followed by a decrease at 48 h post-treatment, with no sperm obtained at 72 h. The effect of Alarelin at 35 µg kg<sup>−1</sup> bw and carp pituitary did not differ at 24 and 48 h post-treatment, whereas 200 µg kg<sup>−1</sup> bw Alarelin was associated with significantly lower spermatozoon concentration 24 h post-treatment compared to carp pituitary, with no difference in milt volume. Higher relative sperm production was observed 48 h after injection of Alarelin at 200 µg kg<sup>−1</sup> bw compared to carp pituitary. Spermatozoon motility was significantly higher in fish receiving Alarelin at 35 µg kg<sup>−1</sup> bw than 200 µg kg<sup>−1</sup> bw. The treatment with optimal effect on inducing spermiation was poly (lactic-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> bw. |
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