Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release

Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg−1 or 200 µg kg−1 body weight to induce spermiation was compared in sterlet Acipenser ruthenus. All hormone treatme...

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Autores principales:	Peter Podhorec, Jindřiška Knowles, Jakub Vysloužil, Sergii Boryshpolets, Kateřina Kubová, Marek Rodina, Vitaliy Kholodnyy, Anatolii Sotnikov, David Gela, Borys Dzyuba
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sperm reproduction sturgeon sustained release Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/0a8f166be9be4144b232e6d1726ce1c6
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spelling	oai:doaj.org-article:0a8f166be9be4144b232e6d1726ce1c62021-11-25T16:21:05ZInduction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release10.3390/ani111133052076-2615https://doaj.org/article/0a8f166be9be4144b232e6d1726ce1c62021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3305https://doaj.org/toc/2076-2615Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> or 200 µg kg<sup>−1</sup> body weight to induce spermiation was compared in sterlet <i>Acipenser ruthenus</i>. All hormone treatments initially increased testosterone and 11-ketotestosterone, with a subsequent decline in testosterone but consistent high levels of 11-ketotestosterone at 48 and 72 h post-treatment. Spermiation did not differ between hormone-treated groups, and was not detected in controls receiving saline solution. Administration of the carp pituitary led to maximum sperm production 24 h post-treatment, followed by a decrease at 48 h post-treatment, with no sperm obtained at 72 h. The effect of Alarelin at 35 µg kg<sup>−1</sup> bw and carp pituitary did not differ at 24 and 48 h post-treatment, whereas 200 µg kg<sup>−1</sup> bw Alarelin was associated with significantly lower spermatozoon concentration 24 h post-treatment compared to carp pituitary, with no difference in milt volume. Higher relative sperm production was observed 48 h after injection of Alarelin at 200 µg kg<sup>−1</sup> bw compared to carp pituitary. Spermatozoon motility was significantly higher in fish receiving Alarelin at 35 µg kg<sup>−1</sup> bw than 200 µg kg<sup>−1</sup> bw. The treatment with optimal effect on inducing spermiation was poly (lactic-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> bw.Peter PodhorecJindřiška KnowlesJakub VysloužilSergii BoryshpoletsKateřina KubováMarek RodinaVitaliy KholodnyyAnatolii SotnikovDavid GelaBorys DzyubaMDPI AGarticlespermreproductionsturgeonsustained releaseVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3305, p 3305 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sperm reproduction sturgeon sustained release Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	sperm reproduction sturgeon sustained release Veterinary medicine SF600-1100 Zoology QL1-991 Peter Podhorec Jindřiška Knowles Jakub Vysloužil Sergii Boryshpolets Kateřina Kubová Marek Rodina Vitaliy Kholodnyy Anatolii Sotnikov David Gela Borys Dzyuba Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
description	Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> or 200 µg kg<sup>−1</sup> body weight to induce spermiation was compared in sterlet <i>Acipenser ruthenus</i>. All hormone treatments initially increased testosterone and 11-ketotestosterone, with a subsequent decline in testosterone but consistent high levels of 11-ketotestosterone at 48 and 72 h post-treatment. Spermiation did not differ between hormone-treated groups, and was not detected in controls receiving saline solution. Administration of the carp pituitary led to maximum sperm production 24 h post-treatment, followed by a decrease at 48 h post-treatment, with no sperm obtained at 72 h. The effect of Alarelin at 35 µg kg<sup>−1</sup> bw and carp pituitary did not differ at 24 and 48 h post-treatment, whereas 200 µg kg<sup>−1</sup> bw Alarelin was associated with significantly lower spermatozoon concentration 24 h post-treatment compared to carp pituitary, with no difference in milt volume. Higher relative sperm production was observed 48 h after injection of Alarelin at 200 µg kg<sup>−1</sup> bw compared to carp pituitary. Spermatozoon motility was significantly higher in fish receiving Alarelin at 35 µg kg<sup>−1</sup> bw than 200 µg kg<sup>−1</sup> bw. The treatment with optimal effect on inducing spermiation was poly (lactic-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg<sup>−1</sup> bw.
format	article
author	Peter Podhorec Jindřiška Knowles Jakub Vysloužil Sergii Boryshpolets Kateřina Kubová Marek Rodina Vitaliy Kholodnyy Anatolii Sotnikov David Gela Borys Dzyuba
author_facet	Peter Podhorec Jindřiška Knowles Jakub Vysloužil Sergii Boryshpolets Kateřina Kubová Marek Rodina Vitaliy Kholodnyy Anatolii Sotnikov David Gela Borys Dzyuba
author_sort	Peter Podhorec
title	Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
title_short	Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
title_full	Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
title_fullStr	Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
title_full_unstemmed	Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release
title_sort	induction of spermiation in sterlet <i>acipenser ruthenus</i> by plga microparticle delivery with sustained alarelin release
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0a8f166be9be4144b232e6d1726ce1c6
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Induction of Spermiation in Sterlet <i>Acipenser ruthenus</i> by PLGA Microparticle Delivery with Sustained Alarelin Release

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