Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress

The membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification a...

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Autores principales:	Pengyun Chen, Hongliang Jian, Fei Wei, Lijiao Gu, Tingli Hu, Xiaoyan Lv, Xiaohao Guo, Jianhua Lu, Liang Ma, Hantao Wang, Aimin Wu, Guangzhi Mao, Shuxun Yu, Hengling Wei
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Gossypium membrane attack complex/perforin (MACPF) cold stress VIGS WGCNA Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0a903bec74d545249d9316189382bff2
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spelling	oai:doaj.org-article:0a903bec74d545249d9316189382bff22021-11-19T15:14:24ZPhylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress1664-462X10.3389/fpls.2021.684227https://doaj.org/article/0a903bec74d545249d9316189382bff22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.684227/fullhttps://doaj.org/toc/1664-462XThe membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification and partial functional verification of the members of the MACPF family. Totally, 100 putative MACPF proteins containing complete MACPF domain were identified from the four cotton species. They were classified into three phylogenetic groups and underwent multifold pressure indicating that selection produced new functional differentiation. Cotton MACPF gene family members expanded mainly through the whole-genome duplication (WGD)/segmental followed by the dispersed. Expression and cis-acting elements analysis revealed that MACPFs play a role in resistance to abiotic stresses, and some selected GhMACPFs were able to respond to the PEG and cold stresses. Co-expression analysis showed that GhMACPFs might interact with valine-glutamine (VQ), WRKY, and Apetala 2 (AP2)/ethylene responsive factor (ERF) domain-containing genes under cold stress. In addition, silencing endogenous GhMACPF26 in cotton by the virus-induced gene silencing (VIGS) method indicated that GhMACPF26 negatively regulates cold tolerance. Our data provided a comprehensive phylogenetic evolutionary view of Gossypium MACPFs. The MACPFs may work together with multiple transcriptional factors and play roles in acclimation to abiotic stress, especially cold stress in cotton.Pengyun ChenHongliang JianFei WeiLijiao GuTingli HuXiaoyan LvXiaohao GuoJianhua LuLiang MaHantao WangAimin WuGuangzhi MaoShuxun YuHengling WeiFrontiers Media S.A.articleGossypiummembrane attack complex/perforin (MACPF)cold stressVIGSWGCNAPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gossypium membrane attack complex/perforin (MACPF) cold stress VIGS WGCNA Plant culture SB1-1110
spellingShingle	Gossypium membrane attack complex/perforin (MACPF) cold stress VIGS WGCNA Plant culture SB1-1110 Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
description	The membrane attack complex/perforin (MACPF) domain-containing proteins are involved in the various developmental processes and in responding to diverse abiotic stress. The function and regulatory network of the MACPF genes are rarely reported in Gossypium spp. We study the detailed identification and partial functional verification of the members of the MACPF family. Totally, 100 putative MACPF proteins containing complete MACPF domain were identified from the four cotton species. They were classified into three phylogenetic groups and underwent multifold pressure indicating that selection produced new functional differentiation. Cotton MACPF gene family members expanded mainly through the whole-genome duplication (WGD)/segmental followed by the dispersed. Expression and cis-acting elements analysis revealed that MACPFs play a role in resistance to abiotic stresses, and some selected GhMACPFs were able to respond to the PEG and cold stresses. Co-expression analysis showed that GhMACPFs might interact with valine-glutamine (VQ), WRKY, and Apetala 2 (AP2)/ethylene responsive factor (ERF) domain-containing genes under cold stress. In addition, silencing endogenous GhMACPF26 in cotton by the virus-induced gene silencing (VIGS) method indicated that GhMACPF26 negatively regulates cold tolerance. Our data provided a comprehensive phylogenetic evolutionary view of Gossypium MACPFs. The MACPFs may work together with multiple transcriptional factors and play roles in acclimation to abiotic stress, especially cold stress in cotton.
format	article
author	Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei
author_facet	Pengyun Chen Hongliang Jian Fei Wei Lijiao Gu Tingli Hu Xiaoyan Lv Xiaohao Guo Jianhua Lu Liang Ma Hantao Wang Aimin Wu Guangzhi Mao Shuxun Yu Hengling Wei
author_sort	Pengyun Chen
title	Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
title_short	Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
title_full	Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
title_fullStr	Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
title_full_unstemmed	Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress
title_sort	phylogenetic analysis of the membrane attack complex/perforin domain-containing proteins in gossypium and the role of ghmacpf26 in cotton under cold stress
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0a903bec74d545249d9316189382bff2
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Phylogenetic Analysis of the Membrane Attack Complex/Perforin Domain-Containing Proteins in Gossypium and the Role of GhMACPF26 in Cotton Under Cold Stress

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