Probiotics protect mice from ovariectomy-induced cortical bone loss.

The gut microbiota (GM) modulates the hosts metabolism and immune system. Probiotic bacteria are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host and can alter the composition of the GM. Germ-free mice have increased bone mass associated...

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Autores principales:	Claes Ohlsson, Cecilia Engdahl, Frida Fåk, Annica Andersson, Sara H Windahl, Helen H Farman, Sofia Movérare-Skrtic, Ulrika Islander, Klara Sjögren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0a923e5ec68047c689340aea5870288f
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spelling	oai:doaj.org-article:0a923e5ec68047c689340aea5870288f2021-11-18T08:27:44ZProbiotics protect mice from ovariectomy-induced cortical bone loss.1932-620310.1371/journal.pone.0092368https://doaj.org/article/0a923e5ec68047c689340aea5870288f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637895/?tool=EBIhttps://doaj.org/toc/1932-6203The gut microbiota (GM) modulates the hosts metabolism and immune system. Probiotic bacteria are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host and can alter the composition of the GM. Germ-free mice have increased bone mass associated with reduced bone resorption indicating that the GM also regulates bone mass. Ovariectomy (ovx) results in bone loss associated with altered immune status. The purpose of this study was to determine if probiotic treatment protects mice from ovx-induced bone loss. Mice were treated with either a single Lactobacillus (L) strain, L. paracasei DSM13434 (L. para) or a mixture of three strains, L. paracasei DSM13434, L. plantarum DSM 15312 and DSM 15313 (L. mix) given in the drinking water during 6 weeks, starting two weeks before ovx. Both the L. para and the L. mix treatment protected mice from ovx-induced cortical bone loss and bone resorption. Cortical bone mineral content was higher in both L. para and L. mix treated ovx mice compared to vehicle (veh) treated ovx mice. Serum levels of the resorption marker C-terminal telopeptides and the urinary fractional excretion of calcium were increased by ovx in the veh treated but not in the L. para or the L. mix treated mice. Probiotic treatment reduced the expression of the two inflammatory cytokines, TNFα and IL-1β, and increased the expression of OPG, a potent inhibitor of osteoclastogenesis, in cortical bone of ovx mice. In addition, ovx decreased the frequency of regulatory T cells in bone marrow of veh treated but not probiotic treated mice. In conclusion, treatment with L. para or the L. mix prevents ovx-induced cortical bone loss. Our findings indicate that these probiotic treatments alter the immune status in bone resulting in attenuated bone resorption in ovx mice.Claes OhlssonCecilia EngdahlFrida FåkAnnica AnderssonSara H WindahlHelen H FarmanSofia Movérare-SkrticUlrika IslanderKlara SjögrenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92368 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claes Ohlsson Cecilia Engdahl Frida Fåk Annica Andersson Sara H Windahl Helen H Farman Sofia Movérare-Skrtic Ulrika Islander Klara Sjögren Probiotics protect mice from ovariectomy-induced cortical bone loss.
description	The gut microbiota (GM) modulates the hosts metabolism and immune system. Probiotic bacteria are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host and can alter the composition of the GM. Germ-free mice have increased bone mass associated with reduced bone resorption indicating that the GM also regulates bone mass. Ovariectomy (ovx) results in bone loss associated with altered immune status. The purpose of this study was to determine if probiotic treatment protects mice from ovx-induced bone loss. Mice were treated with either a single Lactobacillus (L) strain, L. paracasei DSM13434 (L. para) or a mixture of three strains, L. paracasei DSM13434, L. plantarum DSM 15312 and DSM 15313 (L. mix) given in the drinking water during 6 weeks, starting two weeks before ovx. Both the L. para and the L. mix treatment protected mice from ovx-induced cortical bone loss and bone resorption. Cortical bone mineral content was higher in both L. para and L. mix treated ovx mice compared to vehicle (veh) treated ovx mice. Serum levels of the resorption marker C-terminal telopeptides and the urinary fractional excretion of calcium were increased by ovx in the veh treated but not in the L. para or the L. mix treated mice. Probiotic treatment reduced the expression of the two inflammatory cytokines, TNFα and IL-1β, and increased the expression of OPG, a potent inhibitor of osteoclastogenesis, in cortical bone of ovx mice. In addition, ovx decreased the frequency of regulatory T cells in bone marrow of veh treated but not probiotic treated mice. In conclusion, treatment with L. para or the L. mix prevents ovx-induced cortical bone loss. Our findings indicate that these probiotic treatments alter the immune status in bone resulting in attenuated bone resorption in ovx mice.
format	article
author	Claes Ohlsson Cecilia Engdahl Frida Fåk Annica Andersson Sara H Windahl Helen H Farman Sofia Movérare-Skrtic Ulrika Islander Klara Sjögren
author_facet	Claes Ohlsson Cecilia Engdahl Frida Fåk Annica Andersson Sara H Windahl Helen H Farman Sofia Movérare-Skrtic Ulrika Islander Klara Sjögren
author_sort	Claes Ohlsson
title	Probiotics protect mice from ovariectomy-induced cortical bone loss.
title_short	Probiotics protect mice from ovariectomy-induced cortical bone loss.
title_full	Probiotics protect mice from ovariectomy-induced cortical bone loss.
title_fullStr	Probiotics protect mice from ovariectomy-induced cortical bone loss.
title_full_unstemmed	Probiotics protect mice from ovariectomy-induced cortical bone loss.
title_sort	probiotics protect mice from ovariectomy-induced cortical bone loss.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0a923e5ec68047c689340aea5870288f
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Probiotics protect mice from ovariectomy-induced cortical bone loss.

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