Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro

Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro

Abstract Sundarbans Mangrove Ecosystem (SME) is a rich repository of bioactive natural compounds, with immense nutraceutical and therapeutic potential. Till date, the algal population of SME was not explored fully for their anticancer activities. Our aim is to explore the potential of these algal ph...

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Autores principales: Indira Majumder, Subhabrata Paul, Anish Nag, Rita Kundu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/0a96a8b56d7a4d9f88b7136eca02396d
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spelling oai:doaj.org-article:0a96a8b56d7a4d9f88b7136eca02396d2021-12-02T11:43:58ZChloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro10.1038/s41598-020-78592-92045-2322https://doaj.org/article/0a96a8b56d7a4d9f88b7136eca02396d2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78592-9https://doaj.org/toc/2045-2322Abstract Sundarbans Mangrove Ecosystem (SME) is a rich repository of bioactive natural compounds, with immense nutraceutical and therapeutic potential. Till date, the algal population of SME was not explored fully for their anticancer activities. Our aim is to explore the potential of these algal phytochemicals against the proliferation of cervical cancer cells (in vitro) and identify the mode of cell death induced in them. In the present work, the chloroform fraction of marine green alga, Chaetomorpha brachygona was used on SiHa cell line. The algal phytochemicals were identified by GCMS, LCMS and column chromatography and some of the identified compounds, known for significant anticancer activities, have shown strong Bcl-2 binding capacity, as analyzed through molecular docking study. The extract showed cytostatic and cytotoxic activity on SiHa cells. Absence of fragmented DNA, and presence of increased number of acidic vacuoles in the treated cells indicate nonapoptotic cell death. The mode of cell death was likely to be autophagic, as indicated by the enhanced expression of Beclin 1 and LC3BII (considered as autophagic markers) observed by Western blotting. The study indicates that, C. brachygona can successfully inhibit the proliferation of cervical cancer cells in vitro.Indira MajumderSubhabrata PaulAnish NagRita KunduNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Indira Majumder
Subhabrata Paul
Anish Nag
Rita Kundu
Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
description Abstract Sundarbans Mangrove Ecosystem (SME) is a rich repository of bioactive natural compounds, with immense nutraceutical and therapeutic potential. Till date, the algal population of SME was not explored fully for their anticancer activities. Our aim is to explore the potential of these algal phytochemicals against the proliferation of cervical cancer cells (in vitro) and identify the mode of cell death induced in them. In the present work, the chloroform fraction of marine green alga, Chaetomorpha brachygona was used on SiHa cell line. The algal phytochemicals were identified by GCMS, LCMS and column chromatography and some of the identified compounds, known for significant anticancer activities, have shown strong Bcl-2 binding capacity, as analyzed through molecular docking study. The extract showed cytostatic and cytotoxic activity on SiHa cells. Absence of fragmented DNA, and presence of increased number of acidic vacuoles in the treated cells indicate nonapoptotic cell death. The mode of cell death was likely to be autophagic, as indicated by the enhanced expression of Beclin 1 and LC3BII (considered as autophagic markers) observed by Western blotting. The study indicates that, C. brachygona can successfully inhibit the proliferation of cervical cancer cells in vitro.
format article
author Indira Majumder
Subhabrata Paul
Anish Nag
Rita Kundu
author_facet Indira Majumder
Subhabrata Paul
Anish Nag
Rita Kundu
author_sort Indira Majumder
title Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
title_short Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
title_full Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
title_fullStr Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
title_full_unstemmed Chloroform fraction of Chaetomorpha brachygona, a marine green alga from Indian Sundarbans inducing autophagy in cervical cancer cells in vitro
title_sort chloroform fraction of chaetomorpha brachygona, a marine green alga from indian sundarbans inducing autophagy in cervical cancer cells in vitro
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/0a96a8b56d7a4d9f88b7136eca02396d
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AT subhabratapaul chloroformfractionofchaetomorphabrachygonaamarinegreenalgafromindiansundarbansinducingautophagyincervicalcancercellsinvitro
AT anishnag chloroformfractionofchaetomorphabrachygonaamarinegreenalgafromindiansundarbansinducingautophagyincervicalcancercellsinvitro
AT ritakundu chloroformfractionofchaetomorphabrachygonaamarinegreenalgafromindiansundarbansinducingautophagyincervicalcancercellsinvitro
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