Successful cryopreservation of coral larvae using vitrification and laser warming

Abstract Climate change has increased the incidence of coral bleaching events, resulting in the loss of ecosystem function and biodiversity on reefs around the world. As reef degradation accelerates, the need for innovative restoration tools has become acute. Despite past successes with ultra-low te...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jonathan Daly, Nikolas Zuchowicz, C. Isabel Nuñez Lendo, Kanav Khosla, Claire Lager, E. Michael Henley, John Bischof, F. W. Kleinhans, Chiahsin Lin, Esther C. Peters, Mary Hagedorn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Coral Larvae Laser Heating Experiments Cryoprotectant Solution Fungia Scutaria Larval Volume Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0aab132537b34cdbb0e824faf8b2078c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0aab132537b34cdbb0e824faf8b2078c
record_format	dspace
spelling	oai:doaj.org-article:0aab132537b34cdbb0e824faf8b2078c2021-12-02T15:08:57ZSuccessful cryopreservation of coral larvae using vitrification and laser warming10.1038/s41598-018-34035-02045-2322https://doaj.org/article/0aab132537b34cdbb0e824faf8b2078c2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34035-0https://doaj.org/toc/2045-2322Abstract Climate change has increased the incidence of coral bleaching events, resulting in the loss of ecosystem function and biodiversity on reefs around the world. As reef degradation accelerates, the need for innovative restoration tools has become acute. Despite past successes with ultra-low temperature storage of coral sperm to conserve genetic diversity, cryopreservation of larvae has remained elusive due to their large volume, membrane complexity, and sensitivity to chilling injury. Here we show for the first time that coral larvae can survive cryopreservation and resume swimming after warming. Vitrification in a 3.5 M cryoprotectant solution (10% v/v propylene glycol, 5% v/v dimethyl sulfoxide, and 1 M trehalose in phosphate buffered saline) followed by warming at a rate of approximately 4,500,000 °C/min with an infrared laser resulted in up to 43% survival of Fungia scutaria larvae on day 2 post-fertilization. Surviving larvae swam and continued to develop for at least 12 hours after laser-warming. This technology will enable biobanking of coral larvae to secure biodiversity, and, if managed in a high-throughput manner where millions of larvae in a species are frozen at one time, could become an invaluable research and conservation tool to help restore and diversify wild reef habitats.Jonathan DalyNikolas ZuchowiczC. Isabel Nuñez LendoKanav KhoslaClaire LagerE. Michael HenleyJohn BischofF. W. KleinhansChiahsin LinEsther C. PetersMary HagedornNature PortfolioarticleCoral LarvaeLaser Heating ExperimentsCryoprotectant SolutionFungia ScutariaLarval VolumeMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Coral Larvae Laser Heating Experiments Cryoprotectant Solution Fungia Scutaria Larval Volume Medicine R Science Q
spellingShingle	Coral Larvae Laser Heating Experiments Cryoprotectant Solution Fungia Scutaria Larval Volume Medicine R Science Q Jonathan Daly Nikolas Zuchowicz C. Isabel Nuñez Lendo Kanav Khosla Claire Lager E. Michael Henley John Bischof F. W. Kleinhans Chiahsin Lin Esther C. Peters Mary Hagedorn Successful cryopreservation of coral larvae using vitrification and laser warming
description	Abstract Climate change has increased the incidence of coral bleaching events, resulting in the loss of ecosystem function and biodiversity on reefs around the world. As reef degradation accelerates, the need for innovative restoration tools has become acute. Despite past successes with ultra-low temperature storage of coral sperm to conserve genetic diversity, cryopreservation of larvae has remained elusive due to their large volume, membrane complexity, and sensitivity to chilling injury. Here we show for the first time that coral larvae can survive cryopreservation and resume swimming after warming. Vitrification in a 3.5 M cryoprotectant solution (10% v/v propylene glycol, 5% v/v dimethyl sulfoxide, and 1 M trehalose in phosphate buffered saline) followed by warming at a rate of approximately 4,500,000 °C/min with an infrared laser resulted in up to 43% survival of Fungia scutaria larvae on day 2 post-fertilization. Surviving larvae swam and continued to develop for at least 12 hours after laser-warming. This technology will enable biobanking of coral larvae to secure biodiversity, and, if managed in a high-throughput manner where millions of larvae in a species are frozen at one time, could become an invaluable research and conservation tool to help restore and diversify wild reef habitats.
format	article
author	Jonathan Daly Nikolas Zuchowicz C. Isabel Nuñez Lendo Kanav Khosla Claire Lager E. Michael Henley John Bischof F. W. Kleinhans Chiahsin Lin Esther C. Peters Mary Hagedorn
author_facet	Jonathan Daly Nikolas Zuchowicz C. Isabel Nuñez Lendo Kanav Khosla Claire Lager E. Michael Henley John Bischof F. W. Kleinhans Chiahsin Lin Esther C. Peters Mary Hagedorn
author_sort	Jonathan Daly
title	Successful cryopreservation of coral larvae using vitrification and laser warming
title_short	Successful cryopreservation of coral larvae using vitrification and laser warming
title_full	Successful cryopreservation of coral larvae using vitrification and laser warming
title_fullStr	Successful cryopreservation of coral larvae using vitrification and laser warming
title_full_unstemmed	Successful cryopreservation of coral larvae using vitrification and laser warming
title_sort	successful cryopreservation of coral larvae using vitrification and laser warming
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0aab132537b34cdbb0e824faf8b2078c
work_keys_str_mv	AT jonathandaly successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT nikolaszuchowicz successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT cisabelnunezlendo successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT kanavkhosla successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT clairelager successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT emichaelhenley successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT johnbischof successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT fwkleinhans successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT chiahsinlin successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT esthercpeters successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming AT maryhagedorn successfulcryopreservationofcorallarvaeusingvitrificationandlaserwarming
_version_	1718387918353989632

Successful cryopreservation of coral larvae using vitrification and laser warming

Ejemplares similares