Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces

Abstract Exploiting a pure culture strategy to investigate the composition of the human gut microbiota, two novel anaerobes, designated strains AF52-21T and CM04-06T, were isolated from faeces of two healthy Chinese donors and characterized using a polyphasic approach. The two strains were observed...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuanqiang Zou, Xiaoqian Lin, Wenbin Xue, Li Tuo, Ming-Sheng Chen, Xiao-Hui Chen, Cheng-hang Sun, Feina Li, Shao-wei Liu, Ying Dai, Karsten Kristiansen, Liang Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0aae1dc0b5224f769f6fc83115c6f929
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0aae1dc0b5224f769f6fc83115c6f929
record_format	dspace
spelling	oai:doaj.org-article:0aae1dc0b5224f769f6fc83115c6f9292021-12-02T15:57:11ZCharacterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces10.1038/s41598-021-90786-32045-2322https://doaj.org/article/0aae1dc0b5224f769f6fc83115c6f9292021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90786-3https://doaj.org/toc/2045-2322Abstract Exploiting a pure culture strategy to investigate the composition of the human gut microbiota, two novel anaerobes, designated strains AF52-21T and CM04-06T, were isolated from faeces of two healthy Chinese donors and characterized using a polyphasic approach. The two strains were observed to be gram-negative, non-motile, and rod-shaped. Both strains grew optimally at 37 °C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains clustered with species of the genus Faecalibacterium and were most closely related to Faecalibacterium prausnitzii ATCC 27768T with sequence similarity of 97.18% and 96.87%, respectively. The two isolates shared a 16S rRNA gene sequence identity of 98.69%. Draft genome sequencing was performed for strains AF52-21T and CM04-06T, generating genome sizes of 2.85 Mbp and 3.01 Mbp. The calculated average nucleotide identity values between the genomes of the strains AF52-21T and CM04-06T compared to Faecalibacterium prausnitzii ATCC 27768T were 83.20% and 82.54%, respectively, and 90.09% when comparing AF52-21T and CM04-06T. Both values were below the previously proposed species threshold (95–96%), supporting their recognition as novel species in the genus Faecalibacterium. The genomic DNA G + C contents of strains AF52-21T and CM04-06T calculated from genome sequences were 57.77 mol% and 57.51 mol%, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, we conclude that both strains represent two new Faecalibacterium species, for which the names Faecalibacterium butyricigenerans sp. nov. (type strain AF52-21T = CGMCC 1.5206T = DSM 103434T) and Faecalibacterium longum sp. nov. (type strain CM04-06T = CGMCC 1.5208T = DSM 103432T) are proposed.Yuanqiang ZouXiaoqian LinWenbin XueLi TuoMing-Sheng ChenXiao-Hui ChenCheng-hang SunFeina LiShao-wei LiuYing DaiKarsten KristiansenLiang XiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuanqiang Zou Xiaoqian Lin Wenbin Xue Li Tuo Ming-Sheng Chen Xiao-Hui Chen Cheng-hang Sun Feina Li Shao-wei Liu Ying Dai Karsten Kristiansen Liang Xiao Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
description	Abstract Exploiting a pure culture strategy to investigate the composition of the human gut microbiota, two novel anaerobes, designated strains AF52-21T and CM04-06T, were isolated from faeces of two healthy Chinese donors and characterized using a polyphasic approach. The two strains were observed to be gram-negative, non-motile, and rod-shaped. Both strains grew optimally at 37 °C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains clustered with species of the genus Faecalibacterium and were most closely related to Faecalibacterium prausnitzii ATCC 27768T with sequence similarity of 97.18% and 96.87%, respectively. The two isolates shared a 16S rRNA gene sequence identity of 98.69%. Draft genome sequencing was performed for strains AF52-21T and CM04-06T, generating genome sizes of 2.85 Mbp and 3.01 Mbp. The calculated average nucleotide identity values between the genomes of the strains AF52-21T and CM04-06T compared to Faecalibacterium prausnitzii ATCC 27768T were 83.20% and 82.54%, respectively, and 90.09% when comparing AF52-21T and CM04-06T. Both values were below the previously proposed species threshold (95–96%), supporting their recognition as novel species in the genus Faecalibacterium. The genomic DNA G + C contents of strains AF52-21T and CM04-06T calculated from genome sequences were 57.77 mol% and 57.51 mol%, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, we conclude that both strains represent two new Faecalibacterium species, for which the names Faecalibacterium butyricigenerans sp. nov. (type strain AF52-21T = CGMCC 1.5206T = DSM 103434T) and Faecalibacterium longum sp. nov. (type strain CM04-06T = CGMCC 1.5208T = DSM 103432T) are proposed.
format	article
author	Yuanqiang Zou Xiaoqian Lin Wenbin Xue Li Tuo Ming-Sheng Chen Xiao-Hui Chen Cheng-hang Sun Feina Li Shao-wei Liu Ying Dai Karsten Kristiansen Liang Xiao
author_facet	Yuanqiang Zou Xiaoqian Lin Wenbin Xue Li Tuo Ming-Sheng Chen Xiao-Hui Chen Cheng-hang Sun Feina Li Shao-wei Liu Ying Dai Karsten Kristiansen Liang Xiao
author_sort	Yuanqiang Zou
title	Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
title_short	Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
title_full	Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
title_fullStr	Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
title_full_unstemmed	Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
title_sort	characterization and description of faecalibacterium butyricigenerans sp. nov. and f. longum sp. nov., isolated from human faeces
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0aae1dc0b5224f769f6fc83115c6f929
work_keys_str_mv	AT yuanqiangzou characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT xiaoqianlin characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT wenbinxue characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT lituo characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT mingshengchen characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT xiaohuichen characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT chenghangsun characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT feinali characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT shaoweiliu characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT yingdai characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT karstenkristiansen characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces AT liangxiao characterizationanddescriptionoffaecalibacteriumbutyricigeneransspnovandflongumspnovisolatedfromhumanfaeces
_version_	1718385311224954880

Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces

Ejemplares similares