Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells

Abstract A characteristic spatial distribution of the main chromatin fractions is observed in most mammalian cell nuclei, with euchromatin localized in the interior and heterochromatin at the nuclear periphery. It has been shown that interactions of heterochromatin with the nuclear lamina are necess...

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Autores principales:	Joanna Stachecka, Pawel A. Kolodziejski, Magdalena Noak, Izabela Szczerbal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0abcdd9a50d24e639af215733ba4d7a1
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spelling	oai:doaj.org-article:0abcdd9a50d24e639af215733ba4d7a12021-12-02T14:12:45ZAlteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells10.1038/s41598-020-79384-x2045-2322https://doaj.org/article/0abcdd9a50d24e639af215733ba4d7a12021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79384-xhttps://doaj.org/toc/2045-2322Abstract A characteristic spatial distribution of the main chromatin fractions is observed in most mammalian cell nuclei, with euchromatin localized in the interior and heterochromatin at the nuclear periphery. It has been shown that interactions of heterochromatin with the nuclear lamina are necessary to establish this conventional architecture. Adipocytes are specific cells in which a reduction in lamin A/C expression is observed. We hypothesize that the loss of lamin A/C during adipogenic differentiation of mesenchymal stem cells (MSCs) may be associated with the reorganization of the main classes of chromatin in the nucleus. Thus, in this study, we examine the abundance and nuclear distribution of selected heterochromatin (H3K9me3, H3K27me3 and H4K20me3) and euchromatin (H4K8ac, H3K4me3 and H3K9ac) histone marks during in vitro adipogenesis, using the pig as a model organism. We found that not only did the expression of lamin A/C decrease in our differentiation system, but so did the expression of lamin B receptor (LBR). The level of two heterochromatin marks, H3K27me3 and H4K20me3, increased during differentiation, while no changes were observed for H3K9me3. The levels of two euchromatin histone marks, H4K8ac and H3K9ac, were significantly higher in adipocytes than in undifferentiated cells, while the level of H3K4me3 did not change significantly. The spatial distribution of all the examined histone marks altered during in vitro adipogenesis. H3K27me3 and H4K20me3 moved towards the nuclear periphery and H3K9me3 localized preferentially in the intermediate part of adipocyte nuclei. The euchromatin marks H3K9ac and H3K4me3 preferentially occupied the peripheral part of the adipocyte nuclei, while H4K8ac was more evenly distributed in the nuclei of undifferentiated and differentiated cells. Analysis of the nuclear distribution of repetitive sequences has shown their clustering and relocalization toward nuclear periphery during differentiation. Our study shows that dynamic changes in the abundance and nuclear distribution of active and repressive histone marks take place during adipocyte differentiation. Nuclear reorganization of heterochromatin histone marks may allow the maintenance of the nuclear morphology of the adipocytes, in which reduced expression of lamin A/C and LBR is observed.Joanna StacheckaPawel A. KolodziejskiMagdalena NoakIzabela SzczerbalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanna Stachecka Pawel A. Kolodziejski Magdalena Noak Izabela Szczerbal Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
description	Abstract A characteristic spatial distribution of the main chromatin fractions is observed in most mammalian cell nuclei, with euchromatin localized in the interior and heterochromatin at the nuclear periphery. It has been shown that interactions of heterochromatin with the nuclear lamina are necessary to establish this conventional architecture. Adipocytes are specific cells in which a reduction in lamin A/C expression is observed. We hypothesize that the loss of lamin A/C during adipogenic differentiation of mesenchymal stem cells (MSCs) may be associated with the reorganization of the main classes of chromatin in the nucleus. Thus, in this study, we examine the abundance and nuclear distribution of selected heterochromatin (H3K9me3, H3K27me3 and H4K20me3) and euchromatin (H4K8ac, H3K4me3 and H3K9ac) histone marks during in vitro adipogenesis, using the pig as a model organism. We found that not only did the expression of lamin A/C decrease in our differentiation system, but so did the expression of lamin B receptor (LBR). The level of two heterochromatin marks, H3K27me3 and H4K20me3, increased during differentiation, while no changes were observed for H3K9me3. The levels of two euchromatin histone marks, H4K8ac and H3K9ac, were significantly higher in adipocytes than in undifferentiated cells, while the level of H3K4me3 did not change significantly. The spatial distribution of all the examined histone marks altered during in vitro adipogenesis. H3K27me3 and H4K20me3 moved towards the nuclear periphery and H3K9me3 localized preferentially in the intermediate part of adipocyte nuclei. The euchromatin marks H3K9ac and H3K4me3 preferentially occupied the peripheral part of the adipocyte nuclei, while H4K8ac was more evenly distributed in the nuclei of undifferentiated and differentiated cells. Analysis of the nuclear distribution of repetitive sequences has shown their clustering and relocalization toward nuclear periphery during differentiation. Our study shows that dynamic changes in the abundance and nuclear distribution of active and repressive histone marks take place during adipocyte differentiation. Nuclear reorganization of heterochromatin histone marks may allow the maintenance of the nuclear morphology of the adipocytes, in which reduced expression of lamin A/C and LBR is observed.
format	article
author	Joanna Stachecka Pawel A. Kolodziejski Magdalena Noak Izabela Szczerbal
author_facet	Joanna Stachecka Pawel A. Kolodziejski Magdalena Noak Izabela Szczerbal
author_sort	Joanna Stachecka
title	Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
title_short	Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
title_full	Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
title_fullStr	Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
title_full_unstemmed	Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
title_sort	alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0abcdd9a50d24e639af215733ba4d7a1
work_keys_str_mv	AT joannastachecka alterationofactiveandrepressivehistonemarksduringadipogenicdifferentiationofporcinemesenchymalstemcells AT pawelakolodziejski alterationofactiveandrepressivehistonemarksduringadipogenicdifferentiationofporcinemesenchymalstemcells AT magdalenanoak alterationofactiveandrepressivehistonemarksduringadipogenicdifferentiationofporcinemesenchymalstemcells AT izabelaszczerbal alterationofactiveandrepressivehistonemarksduringadipogenicdifferentiationofporcinemesenchymalstemcells
_version_	1718391766222110720

Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells

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