Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model

Transferrins constitute the most important iron regulation system in vertebrates and some invertebrates. Soluble transferrins, such as bovine lactoferrin and hen egg white ovotransferrin, are glycoproteins with a very similar structure with lobes that complex with iron. In this in vitro study, a com...

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Autores principales:	Rebecca Galla, Paride Grisenti, Mahitab Farghali, Laura Saccuman, Patrizia Ferraboschi, Francesca Uberti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ovotransferrin bovine lactoferrin iron metabolism gastro-intestinal barrier iron absorption iron transportation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0abe0470ae474953a0ac2bc098473bb2
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spelling	oai:doaj.org-article:0abe0470ae474953a0ac2bc098473bb22021-11-25T16:48:48ZOvotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model10.3390/biomedicines91115432227-9059https://doaj.org/article/0abe0470ae474953a0ac2bc098473bb22021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1543https://doaj.org/toc/2227-9059Transferrins constitute the most important iron regulation system in vertebrates and some invertebrates. Soluble transferrins, such as bovine lactoferrin and hen egg white ovotransferrin, are glycoproteins with a very similar structure with lobes that complex with iron. In this in vitro study, a comparison of bovine lactoferrin and ovotransferrin was undertaken to confirm the comparability of biological effects. An in vitro gastric barrier model using gastric epithelial cells GTL-16 and an in vitro intestinal barrier model using CaCo-2 cells was employed to evaluate iron absorption and barrier integrity. An analysis of the molecular pathways involving DMT-1 (divalent metal transporter 1), ferritin and ferroportin was also carried out. These in vitro data demonstrate the activity of both 15% saturated and 100% saturated ovotransferrin on the iron regulation system. Compared with the commercial bovine lactoferrin, both 15% saturated and 100% saturated ovotransferrin were found to act in a more physiological manner. Based on these data, it is possible to hypothesise that ovotransferrin may be an excellent candidate for iron supplementation in humans; in particular, 15% saturated ovotransferrin is the overall best performing product. In vivo studies should be performed to confirm this in vitro data.Rebecca GallaParide GrisentiMahitab FarghaliLaura SaccumanPatrizia FerraboschiFrancesca UbertiMDPI AGarticleovotransferrinbovine lactoferriniron metabolismgastro-intestinal barrieriron absorptioniron transportationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1543, p 1543 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovotransferrin bovine lactoferrin iron metabolism gastro-intestinal barrier iron absorption iron transportation Biology (General) QH301-705.5
spellingShingle	ovotransferrin bovine lactoferrin iron metabolism gastro-intestinal barrier iron absorption iron transportation Biology (General) QH301-705.5 Rebecca Galla Paride Grisenti Mahitab Farghali Laura Saccuman Patrizia Ferraboschi Francesca Uberti Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
description	Transferrins constitute the most important iron regulation system in vertebrates and some invertebrates. Soluble transferrins, such as bovine lactoferrin and hen egg white ovotransferrin, are glycoproteins with a very similar structure with lobes that complex with iron. In this in vitro study, a comparison of bovine lactoferrin and ovotransferrin was undertaken to confirm the comparability of biological effects. An in vitro gastric barrier model using gastric epithelial cells GTL-16 and an in vitro intestinal barrier model using CaCo-2 cells was employed to evaluate iron absorption and barrier integrity. An analysis of the molecular pathways involving DMT-1 (divalent metal transporter 1), ferritin and ferroportin was also carried out. These in vitro data demonstrate the activity of both 15% saturated and 100% saturated ovotransferrin on the iron regulation system. Compared with the commercial bovine lactoferrin, both 15% saturated and 100% saturated ovotransferrin were found to act in a more physiological manner. Based on these data, it is possible to hypothesise that ovotransferrin may be an excellent candidate for iron supplementation in humans; in particular, 15% saturated ovotransferrin is the overall best performing product. In vivo studies should be performed to confirm this in vitro data.
format	article
author	Rebecca Galla Paride Grisenti Mahitab Farghali Laura Saccuman Patrizia Ferraboschi Francesca Uberti
author_facet	Rebecca Galla Paride Grisenti Mahitab Farghali Laura Saccuman Patrizia Ferraboschi Francesca Uberti
author_sort	Rebecca Galla
title	Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
title_short	Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
title_full	Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
title_fullStr	Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
title_full_unstemmed	Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model
title_sort	ovotransferrin supplementation improves the iron absorption: an in vitro gastro-intestinal model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0abe0470ae474953a0ac2bc098473bb2
work_keys_str_mv	AT rebeccagalla ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel AT paridegrisenti ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel AT mahitabfarghali ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel AT laurasaccuman ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel AT patriziaferraboschi ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel AT francescauberti ovotransferrinsupplementationimprovestheironabsorptionaninvitrogastrointestinalmodel
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Ovotransferrin Supplementation Improves the Iron Absorption: An In Vitro Gastro-Intestinal Model

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