Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.

Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not b...

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Autores principales:	Lærke Münter Lassen, Agnieszka Zygadlo Nielsen, Carl Erik Olsen, Wojciech Bialek, Kenneth Jensen, Birger Lindberg Møller, Poul Erik Jensen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0abf7c9d61ac4be282938be261272d5e
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spelling	oai:doaj.org-article:0abf7c9d61ac4be282938be261272d5e2021-11-25T06:08:30ZAnchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.1932-620310.1371/journal.pone.0102184https://doaj.org/article/0abf7c9d61ac4be282938be261272d5e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25025215/?tool=EBIhttps://doaj.org/toc/1932-6203Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not be feasible. Here, we take advantage of the reducing equivalents generated in photosynthesis in developing an approach for producing plant bioactive natural compounds in a photosynthetic microorganism by functionally coupling a biosynthetic enzyme to photosystem I. This enables driving of the enzymatic reactions with electrons extracted from the photosynthetic electron transport chain. As a proof of concept, we have genetically fused the soluble catalytic domain of the cytochrome P450 CYP79A1, originating from the endoplasmic reticulum membranes of Sorghum bicolor, to a photosystem I subunit in the cyanobacterium Synechococcus sp. PCC 7002, thereby targeting it to the thylakoids. The engineered enzyme showed light-driven activity both in vivo and in vitro, demonstrating the possibility to achieve light-driven biosynthesis of high-value plant specialized metabolites in cyanobacteria.Lærke Münter LassenAgnieszka Zygadlo NielsenCarl Erik OlsenWojciech BialekKenneth JensenBirger Lindberg MøllerPoul Erik JensenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102184 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lærke Münter Lassen Agnieszka Zygadlo Nielsen Carl Erik Olsen Wojciech Bialek Kenneth Jensen Birger Lindberg Møller Poul Erik Jensen Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
description	Plants produce an immense variety of specialized metabolites, many of which are of high value as their bioactive properties make them useful as for instance pharmaceuticals. The compounds are often produced at low levels in the plant, and due to their complex structures, chemical synthesis may not be feasible. Here, we take advantage of the reducing equivalents generated in photosynthesis in developing an approach for producing plant bioactive natural compounds in a photosynthetic microorganism by functionally coupling a biosynthetic enzyme to photosystem I. This enables driving of the enzymatic reactions with electrons extracted from the photosynthetic electron transport chain. As a proof of concept, we have genetically fused the soluble catalytic domain of the cytochrome P450 CYP79A1, originating from the endoplasmic reticulum membranes of Sorghum bicolor, to a photosystem I subunit in the cyanobacterium Synechococcus sp. PCC 7002, thereby targeting it to the thylakoids. The engineered enzyme showed light-driven activity both in vivo and in vitro, demonstrating the possibility to achieve light-driven biosynthesis of high-value plant specialized metabolites in cyanobacteria.
format	article
author	Lærke Münter Lassen Agnieszka Zygadlo Nielsen Carl Erik Olsen Wojciech Bialek Kenneth Jensen Birger Lindberg Møller Poul Erik Jensen
author_facet	Lærke Münter Lassen Agnieszka Zygadlo Nielsen Carl Erik Olsen Wojciech Bialek Kenneth Jensen Birger Lindberg Møller Poul Erik Jensen
author_sort	Lærke Münter Lassen
title	Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
title_short	Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
title_full	Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
title_fullStr	Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
title_full_unstemmed	Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.
title_sort	anchoring a plant cytochrome p450 via psam to the thylakoids in synechococcus sp. pcc 7002: evidence for light-driven biosynthesis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0abf7c9d61ac4be282938be261272d5e
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Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: evidence for light-driven biosynthesis.

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