Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile

Abstract Plant grafting has been practiced widely in horticulture and proved as a useful tool in science. However, the mechanisms of graft healing or graft incompatibility remain poorly understood. In this study, Litchi chinensis cv. ‘Jingganghongnuo’ homograft (‘J/J’) and ‘Jingganghongnuo’/‘zhuangy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhe Chen, Jietang Zhao, Fuchu Hu, Yonghua Qin, Xianghe Wang, Guibing Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ac04b8798584179a952bf364a3c3ff4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0ac04b8798584179a952bf364a3c3ff4
record_format	dspace
spelling	oai:doaj.org-article:0ac04b8798584179a952bf364a3c3ff42021-12-02T12:32:28ZTranscriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile10.1038/s41598-017-04328-x2045-2322https://doaj.org/article/0ac04b8798584179a952bf364a3c3ff42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04328-xhttps://doaj.org/toc/2045-2322Abstract Plant grafting has been practiced widely in horticulture and proved as a useful tool in science. However, the mechanisms of graft healing or graft incompatibility remain poorly understood. In this study, Litchi chinensis cv. ‘Jingganghongnuo’ homograft (‘J/J’) and ‘Jingganghongnuo’/‘zhuangyuanhong’ heterograft (‘J/Z’) as compatible and incompatible combination, respectively, was used to study transcriptional changes between incompatible and compatible graft during graft union formation. Anatomical observation indicated that three stages (2 h, 14 d and 21 d after grafting) were critical for graft union formation and selected for high-throughput sequencing. Results indicated 6060 DEGs were differentially expressed in the compatible combination and 5267 DEGs exhibiting in the incompatible one. KEGG pathway enrichment analysis revealed that DEGs were involved in metabolism, wound response, phenylpropanoid biosynthesis and plant hormone signal transduction. The expression of 9 DEGs annotated in auxin pathway was up-regulated in compatible combination than that in incompatible combination. The IAA concentration confirmed that the IAA might promote the graft compatibility. In addition, 13 DEGs related to lignin biosynthesis were differentially expressed during graft healing process. Overall, our results provide abundant sequence resources for studying mechanisms underlying graft compatibility and establish a platform for further studies of litchi and other evergreen fruit trees.Zhe ChenJietang ZhaoFuchu HuYonghua QinXianghe WangGuibing HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhe Chen Jietang Zhao Fuchu Hu Yonghua Qin Xianghe Wang Guibing Hu Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
description	Abstract Plant grafting has been practiced widely in horticulture and proved as a useful tool in science. However, the mechanisms of graft healing or graft incompatibility remain poorly understood. In this study, Litchi chinensis cv. ‘Jingganghongnuo’ homograft (‘J/J’) and ‘Jingganghongnuo’/‘zhuangyuanhong’ heterograft (‘J/Z’) as compatible and incompatible combination, respectively, was used to study transcriptional changes between incompatible and compatible graft during graft union formation. Anatomical observation indicated that three stages (2 h, 14 d and 21 d after grafting) were critical for graft union formation and selected for high-throughput sequencing. Results indicated 6060 DEGs were differentially expressed in the compatible combination and 5267 DEGs exhibiting in the incompatible one. KEGG pathway enrichment analysis revealed that DEGs were involved in metabolism, wound response, phenylpropanoid biosynthesis and plant hormone signal transduction. The expression of 9 DEGs annotated in auxin pathway was up-regulated in compatible combination than that in incompatible combination. The IAA concentration confirmed that the IAA might promote the graft compatibility. In addition, 13 DEGs related to lignin biosynthesis were differentially expressed during graft healing process. Overall, our results provide abundant sequence resources for studying mechanisms underlying graft compatibility and establish a platform for further studies of litchi and other evergreen fruit trees.
format	article
author	Zhe Chen Jietang Zhao Fuchu Hu Yonghua Qin Xianghe Wang Guibing Hu
author_facet	Zhe Chen Jietang Zhao Fuchu Hu Yonghua Qin Xianghe Wang Guibing Hu
author_sort	Zhe Chen
title	Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
title_short	Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
title_full	Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
title_fullStr	Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
title_full_unstemmed	Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile
title_sort	transcriptome changes between compatible and incompatible graft combination of litchi chinensis by digital gene expression profile
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0ac04b8798584179a952bf364a3c3ff4
work_keys_str_mv	AT zhechen transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile AT jietangzhao transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile AT fuchuhu transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile AT yonghuaqin transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile AT xianghewang transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile AT guibinghu transcriptomechangesbetweencompatibleandincompatiblegraftcombinationoflitchichinensisbydigitalgeneexpressionprofile
_version_	1718394019643392000

Transcriptome changes between compatible and incompatible graft combination of Litchi chinensis by digital gene expression profile

Ejemplares similares