Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

To elucidate the mechanism underlying increased fatty acid and astaxanthin accumulation in Haematococcus pluvialis, transcriptome analysis was performed to gain insights into the multiple defensive systems elicited by salicylic acid combined with sodium acetate (SAHS) stresses with a time course. To...

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Autores principales:	Qunju Hu, Mingjian Song, Danqiong Huang, Zhangli Hu, Yan Wu, Chaogang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	salicylic acid and sodium acetate stresses Haematococcus pluvialis transcriptomic analysis metabolic coordination astaxanthin biosynthesis fatty acids biosynthesis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0ac7bf8728ee45f482b276ee8114623d
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spelling	oai:doaj.org-article:0ac7bf8728ee45f482b276ee8114623d2021-11-19T04:52:17ZHaematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress1664-462X10.3389/fpls.2021.763742https://doaj.org/article/0ac7bf8728ee45f482b276ee8114623d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.763742/fullhttps://doaj.org/toc/1664-462XTo elucidate the mechanism underlying increased fatty acid and astaxanthin accumulation in Haematococcus pluvialis, transcriptome analysis was performed to gain insights into the multiple defensive systems elicited by salicylic acid combined with sodium acetate (SAHS) stresses with a time course. Totally, 112,886 unigenes and 61,323 non-repeat genes were identified, and genes involved in carbon metabolism, primary and secondary metabolism, and immune system responses were identified. The results revealed that SA and NaAC provide both energy and precursors to improve cell growth of H. pluvialis and enhance carbon assimilation, astaxanthin, and fatty acids production in this microalga with an effective mechanism. Interestingly, SA was considered to play an important role in lowering transcriptional activity of the fatty acid and astaxanthin biosynthesis genes through self-protection metabolism in H. pluvialis, leading to its adaption to HS stress and finally avoiding massive cell death. Moreover, positive correlations between 15 key genes involved in astaxanthin and fatty acid biosynthesis pathways were found, revealing cooperative relation between these pathways at the transcription level. These results not only enriched our knowledge of the astaxanthin accumulation mechanism in H. pluvialis but also provided a new view on increasing astaxanthin production in H. pluvialis by a moderate and sustainable way in the future.Qunju HuQunju HuMingjian SongDanqiong HuangZhangli HuYan WuChaogang WangFrontiers Media S.A.articlesalicylic acid and sodium acetate stressesHaematococcus pluvialistranscriptomic analysismetabolic coordinationastaxanthin biosynthesisfatty acids biosynthesisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	salicylic acid and sodium acetate stresses Haematococcus pluvialis transcriptomic analysis metabolic coordination astaxanthin biosynthesis fatty acids biosynthesis Plant culture SB1-1110
spellingShingle	salicylic acid and sodium acetate stresses Haematococcus pluvialis transcriptomic analysis metabolic coordination astaxanthin biosynthesis fatty acids biosynthesis Plant culture SB1-1110 Qunju Hu Qunju Hu Mingjian Song Danqiong Huang Zhangli Hu Yan Wu Chaogang Wang Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
description	To elucidate the mechanism underlying increased fatty acid and astaxanthin accumulation in Haematococcus pluvialis, transcriptome analysis was performed to gain insights into the multiple defensive systems elicited by salicylic acid combined with sodium acetate (SAHS) stresses with a time course. Totally, 112,886 unigenes and 61,323 non-repeat genes were identified, and genes involved in carbon metabolism, primary and secondary metabolism, and immune system responses were identified. The results revealed that SA and NaAC provide both energy and precursors to improve cell growth of H. pluvialis and enhance carbon assimilation, astaxanthin, and fatty acids production in this microalga with an effective mechanism. Interestingly, SA was considered to play an important role in lowering transcriptional activity of the fatty acid and astaxanthin biosynthesis genes through self-protection metabolism in H. pluvialis, leading to its adaption to HS stress and finally avoiding massive cell death. Moreover, positive correlations between 15 key genes involved in astaxanthin and fatty acid biosynthesis pathways were found, revealing cooperative relation between these pathways at the transcription level. These results not only enriched our knowledge of the astaxanthin accumulation mechanism in H. pluvialis but also provided a new view on increasing astaxanthin production in H. pluvialis by a moderate and sustainable way in the future.
format	article
author	Qunju Hu Qunju Hu Mingjian Song Danqiong Huang Zhangli Hu Yan Wu Chaogang Wang
author_facet	Qunju Hu Qunju Hu Mingjian Song Danqiong Huang Zhangli Hu Yan Wu Chaogang Wang
author_sort	Qunju Hu
title	Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
title_short	Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
title_full	Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
title_fullStr	Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
title_full_unstemmed	Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress
title_sort	haematococcus pluvialis accumulated lipid and astaxanthin in a moderate and sustainable way by the self-protection mechanism of salicylic acid under sodium acetate stress
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0ac7bf8728ee45f482b276ee8114623d
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Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

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