Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)

Abstract Background Genetically modified (GM) crops have become a common tool for managing insect pests of economic importance. However, before their commercialization, bio-safety evaluation on non-target arthropods such as pollinators and biological control agents is a pre-requisite to save service...

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Autores principales:	Intazar Ali, Shuai Zhang, Asif Sajjad, Ghulam Khaliq, Muhammad Basit, Ghulam Ali Bugti, Hafiz Tassawar Abbas, Naeem Abbas, Jin-Jie Cui
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Bio-safety evaluation Dietary exposure test Propylea japonica Vip3Aa protein Agriculture S
Acceso en línea:	https://doaj.org/article/0ac8935cde5740d4843af3eb20408038
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spelling	oai:doaj.org-article:0ac8935cde5740d4843af3eb204080382021-11-28T12:06:16ZEvaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)10.1186/s41938-021-00491-62536-9342https://doaj.org/article/0ac8935cde5740d4843af3eb204080382021-11-01T00:00:00Zhttps://doi.org/10.1186/s41938-021-00491-6https://doaj.org/toc/2536-9342Abstract Background Genetically modified (GM) crops have become a common tool for managing insect pests of economic importance. However, before their commercialization, bio-safety evaluation on non-target arthropods such as pollinators and biological control agents is a pre-requisite to save services rendered by them in agro-ecosystem. Results In the present experiment, the potential adverse effects of Bt protein (Vip3Aa) were studied on the life table parameters (pupation and emergence rates, 7-day larval weights, larval development time, and adult weight) of a predatory beetle, Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) in the laboratory. P. japonica was exposed to Bt protein incorporated in synthetic diet at the rate of 200 µg/g of fresh weight of diet. Temporal stability, bioactivity and the uptake of insecticidal protein were confirmed by enzyme-linked immunosorbent assay and a sensitive insect bioassay. The results indicated that pupation and adult emergence rates did not differ significantly from that of negative control (pure synthetic diet). Similarly, the fresh weight of male and female adults emerged in Vip3Aa treatment (2.87 mg and 3.86 mg, respectively) did not vary significantly from that of the negative control treatment (2.66 mg, 3.75 mg). However, the time taken by the larvae to complete their development (12.70 days) was significantly extended than that of the negative control (10.21 days). In contrast, the tested life-table parameters of P. japonica were adversely affected when fed on synthetic diet containing boric acid (positive control) indicating experimental fitness to detect adverse effects. Conclusions On the basis of the results, it is suggested that GM crops expressing Bt protein (Vip3Aa) are safe for non-target insects such as P. japonica and are compatible with other biological programs for the integrated management of insect pests.Intazar AliShuai ZhangAsif SajjadGhulam KhaliqMuhammad BasitGhulam Ali BugtiHafiz Tassawar AbbasNaeem AbbasJin-Jie CuiSpringerOpenarticleBio-safety evaluationDietary exposure testPropylea japonicaVip3Aa proteinAgricultureSENEgyptian Journal of Biological Pest Control, Vol 31, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bio-safety evaluation Dietary exposure test Propylea japonica Vip3Aa protein Agriculture S
spellingShingle	Bio-safety evaluation Dietary exposure test Propylea japonica Vip3Aa protein Agriculture S Intazar Ali Shuai Zhang Asif Sajjad Ghulam Khaliq Muhammad Basit Ghulam Ali Bugti Hafiz Tassawar Abbas Naeem Abbas Jin-Jie Cui Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
description	Abstract Background Genetically modified (GM) crops have become a common tool for managing insect pests of economic importance. However, before their commercialization, bio-safety evaluation on non-target arthropods such as pollinators and biological control agents is a pre-requisite to save services rendered by them in agro-ecosystem. Results In the present experiment, the potential adverse effects of Bt protein (Vip3Aa) were studied on the life table parameters (pupation and emergence rates, 7-day larval weights, larval development time, and adult weight) of a predatory beetle, Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) in the laboratory. P. japonica was exposed to Bt protein incorporated in synthetic diet at the rate of 200 µg/g of fresh weight of diet. Temporal stability, bioactivity and the uptake of insecticidal protein were confirmed by enzyme-linked immunosorbent assay and a sensitive insect bioassay. The results indicated that pupation and adult emergence rates did not differ significantly from that of negative control (pure synthetic diet). Similarly, the fresh weight of male and female adults emerged in Vip3Aa treatment (2.87 mg and 3.86 mg, respectively) did not vary significantly from that of the negative control treatment (2.66 mg, 3.75 mg). However, the time taken by the larvae to complete their development (12.70 days) was significantly extended than that of the negative control (10.21 days). In contrast, the tested life-table parameters of P. japonica were adversely affected when fed on synthetic diet containing boric acid (positive control) indicating experimental fitness to detect adverse effects. Conclusions On the basis of the results, it is suggested that GM crops expressing Bt protein (Vip3Aa) are safe for non-target insects such as P. japonica and are compatible with other biological programs for the integrated management of insect pests.
format	article
author	Intazar Ali Shuai Zhang Asif Sajjad Ghulam Khaliq Muhammad Basit Ghulam Ali Bugti Hafiz Tassawar Abbas Naeem Abbas Jin-Jie Cui
author_facet	Intazar Ali Shuai Zhang Asif Sajjad Ghulam Khaliq Muhammad Basit Ghulam Ali Bugti Hafiz Tassawar Abbas Naeem Abbas Jin-Jie Cui
author_sort	Intazar Ali
title	Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
title_short	Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
title_full	Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
title_fullStr	Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
title_full_unstemmed	Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
title_sort	evaluating the effects of bt protein vip3aa on immature propylea japonica (thunberg) (coleoptera: coccinellidae)
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/0ac8935cde5740d4843af3eb20408038
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Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)

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