Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.

Complex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative changes. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objecti...

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Autores principales:	Omer Yuval, Yael Iosilevskii, Anna Meledin, Benjamin Podbilewicz, Tom Shemesh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0acfbdeadd3f47eb9e50a23a84e01936
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spelling	oai:doaj.org-article:0acfbdeadd3f47eb9e50a23a84e019362021-12-02T19:57:24ZNeuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.1553-734X1553-735810.1371/journal.pcbi.1009185https://doaj.org/article/0acfbdeadd3f47eb9e50a23a84e019362021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009185https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Complex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative changes. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objective and automated analyses of PVD morphology are missing. Here, we present a method for neuronal feature extraction, based on deep-learning and fitting algorithms. The extracted neuronal architecture is represented by a database of structural elements for abstracted analysis. We obtain excellent automatic tracing of PVD trees and uncover that dendritic junctions are unevenly distributed. Surprisingly, these junctions are three-way-symmetrical on average, while dendritic processes are arranged orthogonally. We quantify the effect of mutation in git-1, a regulator of dendritic spine formation, on PVD morphology and discover a localized reduction in junctions. Our findings shed new light on PVD architecture, demonstrating the effectiveness of our objective analyses of dendritic morphology and suggest molecular control mechanisms.Omer YuvalYael IosilevskiiAnna MeledinBenjamin PodbilewiczTom ShemeshPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 7, p e1009185 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Omer Yuval Yael Iosilevskii Anna Meledin Benjamin Podbilewicz Tom Shemesh Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
description	Complex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative changes. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objective and automated analyses of PVD morphology are missing. Here, we present a method for neuronal feature extraction, based on deep-learning and fitting algorithms. The extracted neuronal architecture is represented by a database of structural elements for abstracted analysis. We obtain excellent automatic tracing of PVD trees and uncover that dendritic junctions are unevenly distributed. Surprisingly, these junctions are three-way-symmetrical on average, while dendritic processes are arranged orthogonally. We quantify the effect of mutation in git-1, a regulator of dendritic spine formation, on PVD morphology and discover a localized reduction in junctions. Our findings shed new light on PVD architecture, demonstrating the effectiveness of our objective analyses of dendritic morphology and suggest molecular control mechanisms.
format	article
author	Omer Yuval Yael Iosilevskii Anna Meledin Benjamin Podbilewicz Tom Shemesh
author_facet	Omer Yuval Yael Iosilevskii Anna Meledin Benjamin Podbilewicz Tom Shemesh
author_sort	Omer Yuval
title	Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
title_short	Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
title_full	Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
title_fullStr	Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
title_full_unstemmed	Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.
title_sort	neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in c. elegans.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0acfbdeadd3f47eb9e50a23a84e01936
work_keys_str_mv	AT omeryuval neurontracingandquantitativeanalysesofdendriticarchitecturerevealsymmetricalthreewayjunctionsandphenotypesofgit1incelegans AT yaeliosilevskii neurontracingandquantitativeanalysesofdendriticarchitecturerevealsymmetricalthreewayjunctionsandphenotypesofgit1incelegans AT annameledin neurontracingandquantitativeanalysesofdendriticarchitecturerevealsymmetricalthreewayjunctionsandphenotypesofgit1incelegans AT benjaminpodbilewicz neurontracingandquantitativeanalysesofdendriticarchitecturerevealsymmetricalthreewayjunctionsandphenotypesofgit1incelegans AT tomshemesh neurontracingandquantitativeanalysesofdendriticarchitecturerevealsymmetricalthreewayjunctionsandphenotypesofgit1incelegans
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Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans.

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