Tamoxifen-independent recombination in the RIP-CreER mouse.

<h4>Background</h4>The inducible Cre-lox system is a valuable tool to study gene function in a spatial and time restricted fashion in mouse models. This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competiti...

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Autores principales:	Yanmei Liu, Jakob Suckale, Jimmy Masjkur, Maria Grazia Magro, Anja Steffen, Konstantinos Anastassiadis, Michele Solimena
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ad63619665c4295884bd2126bc69adf
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spelling	oai:doaj.org-article:0ad63619665c4295884bd2126bc69adf2021-11-18T07:02:57ZTamoxifen-independent recombination in the RIP-CreER mouse.1932-620310.1371/journal.pone.0013533https://doaj.org/article/0ad63619665c4295884bd2126bc69adf2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21063464/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The inducible Cre-lox system is a valuable tool to study gene function in a spatial and time restricted fashion in mouse models. This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competitive estrogen receptor ligand, tamoxifen. The RIP-CreER mouse (Tg (Ins2-cre/Esr1) 1Dam) is among the few available β-cell specific CreER mouse lines and thus it has been often used to manipulate gene expression in the insulin-producing cells of the endocrine pancreas.<h4>Principal findings</h4>Here, we report the detection of tamoxifen-independent Cre activity as early as 2 months of age in RIP-CreER mice crossed with three distinct reporter strains.<h4>Significance</h4>Evidence of Cre-mediated recombination of floxed alleles even in the absence of tamoxifen administration should warrant cautious use of this mouse for the study of pancreatic β-cells.Yanmei LiuJakob SuckaleJimmy MasjkurMaria Grazia MagroAnja SteffenKonstantinos AnastassiadisMichele SolimenaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13533 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanmei Liu Jakob Suckale Jimmy Masjkur Maria Grazia Magro Anja Steffen Konstantinos Anastassiadis Michele Solimena Tamoxifen-independent recombination in the RIP-CreER mouse.
description	<h4>Background</h4>The inducible Cre-lox system is a valuable tool to study gene function in a spatial and time restricted fashion in mouse models. This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competitive estrogen receptor ligand, tamoxifen. The RIP-CreER mouse (Tg (Ins2-cre/Esr1) 1Dam) is among the few available β-cell specific CreER mouse lines and thus it has been often used to manipulate gene expression in the insulin-producing cells of the endocrine pancreas.<h4>Principal findings</h4>Here, we report the detection of tamoxifen-independent Cre activity as early as 2 months of age in RIP-CreER mice crossed with three distinct reporter strains.<h4>Significance</h4>Evidence of Cre-mediated recombination of floxed alleles even in the absence of tamoxifen administration should warrant cautious use of this mouse for the study of pancreatic β-cells.
format	article
author	Yanmei Liu Jakob Suckale Jimmy Masjkur Maria Grazia Magro Anja Steffen Konstantinos Anastassiadis Michele Solimena
author_facet	Yanmei Liu Jakob Suckale Jimmy Masjkur Maria Grazia Magro Anja Steffen Konstantinos Anastassiadis Michele Solimena
author_sort	Yanmei Liu
title	Tamoxifen-independent recombination in the RIP-CreER mouse.
title_short	Tamoxifen-independent recombination in the RIP-CreER mouse.
title_full	Tamoxifen-independent recombination in the RIP-CreER mouse.
title_fullStr	Tamoxifen-independent recombination in the RIP-CreER mouse.
title_full_unstemmed	Tamoxifen-independent recombination in the RIP-CreER mouse.
title_sort	tamoxifen-independent recombination in the rip-creer mouse.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/0ad63619665c4295884bd2126bc69adf
work_keys_str_mv	AT yanmeiliu tamoxifenindependentrecombinationintheripcreermouse AT jakobsuckale tamoxifenindependentrecombinationintheripcreermouse AT jimmymasjkur tamoxifenindependentrecombinationintheripcreermouse AT mariagraziamagro tamoxifenindependentrecombinationintheripcreermouse AT anjasteffen tamoxifenindependentrecombinationintheripcreermouse AT konstantinosanastassiadis tamoxifenindependentrecombinationintheripcreermouse AT michelesolimena tamoxifenindependentrecombinationintheripcreermouse
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Tamoxifen-independent recombination in the RIP-CreER mouse.

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