The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans

Abstract Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to tradition...

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Autores principales:	Maria G. M. Mørch, Katrine V. Møller, Marianne O. Hesselager, Rikke H. Harders, Caroline L. Kidmose, Therese Buhl, Kurt Fuursted, Emøke Bendixen, Chong Shen, Lotte G. Christensen, Charlotte H. Poulsen, Anders Olsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ad88038b6734484a3398d22a1811c97
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spelling	oai:doaj.org-article:0ad88038b6734484a3398d22a1811c972021-12-02T15:45:31ZThe TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans10.1038/s41598-021-89831-y2045-2322https://doaj.org/article/0ad88038b6734484a3398d22a1811c972021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89831-yhttps://doaj.org/toc/2045-2322Abstract Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to traditional antibiotics. Here, we use the nematode Caenorhabditis elegans (C. elegans) to screen a library of different lactobacilli to identify potential probiotic bacteria and characterize their mechanisms of action. We show that pretreatment with the Lactobacillus spp. Lb21 increases lifespan of C. elegans and results in resistance towards pathogenic methicillin-resistant Staphylococcus aureus (MRSA). Using genetic analysis, we find that Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway in C. elegans. This response is evolutionarily conserved as we find that Lb21 also induces the TGF-β pathway in porcine epithelial cells. We further characterize the host responses in an unbiased proteome analysis and identify 474 proteins regulated in worms fed Lb21 compared to control food. These include fatty acid CoA synthetase ACS-22, aspartic protease ASP-6 and vitellogenin VIT-2 which are important for Lb21-mediated MRSA resistance. Thus, Lb21 exerts its probiotic effect on C. elegans in a multifactorial manner. In summary, our study establishes a mechanistic basis for the antimicrobial potential of lactobacilli.Maria G. M. MørchKatrine V. MøllerMarianne O. HesselagerRikke H. HardersCaroline L. KidmoseTherese BuhlKurt FuurstedEmøke BendixenChong ShenLotte G. ChristensenCharlotte H. PoulsenAnders OlsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria G. M. Mørch Katrine V. Møller Marianne O. Hesselager Rikke H. Harders Caroline L. Kidmose Therese Buhl Kurt Fuursted Emøke Bendixen Chong Shen Lotte G. Christensen Charlotte H. Poulsen Anders Olsen The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
description	Abstract Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to traditional antibiotics. Here, we use the nematode Caenorhabditis elegans (C. elegans) to screen a library of different lactobacilli to identify potential probiotic bacteria and characterize their mechanisms of action. We show that pretreatment with the Lactobacillus spp. Lb21 increases lifespan of C. elegans and results in resistance towards pathogenic methicillin-resistant Staphylococcus aureus (MRSA). Using genetic analysis, we find that Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway in C. elegans. This response is evolutionarily conserved as we find that Lb21 also induces the TGF-β pathway in porcine epithelial cells. We further characterize the host responses in an unbiased proteome analysis and identify 474 proteins regulated in worms fed Lb21 compared to control food. These include fatty acid CoA synthetase ACS-22, aspartic protease ASP-6 and vitellogenin VIT-2 which are important for Lb21-mediated MRSA resistance. Thus, Lb21 exerts its probiotic effect on C. elegans in a multifactorial manner. In summary, our study establishes a mechanistic basis for the antimicrobial potential of lactobacilli.
format	article
author	Maria G. M. Mørch Katrine V. Møller Marianne O. Hesselager Rikke H. Harders Caroline L. Kidmose Therese Buhl Kurt Fuursted Emøke Bendixen Chong Shen Lotte G. Christensen Charlotte H. Poulsen Anders Olsen
author_facet	Maria G. M. Mørch Katrine V. Møller Marianne O. Hesselager Rikke H. Harders Caroline L. Kidmose Therese Buhl Kurt Fuursted Emøke Bendixen Chong Shen Lotte G. Christensen Charlotte H. Poulsen Anders Olsen
author_sort	Maria G. M. Mørch
title	The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
title_short	The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
title_full	The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
title_fullStr	The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
title_full_unstemmed	The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans
title_sort	tgf-β ligand dbl-1 is a key player in a multifaceted probiotic protection against mrsa in c. elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ad88038b6734484a3398d22a1811c97
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The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans

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