Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment

SUMMARY: Foodborne pathogens, such as Salmonella are problematic in food processing environments, and understanding the means of persistence is critical in the development of effective control measures. This study determined the antimicrobial tolerance of Salmonella isolates from the processing envi...

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Autores principales:	T. Obe, R. Nannapaneni, W. Schilling, L. Zhang, A. Kiess
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Salmonella biofilm antibiotic resistance poultry processing Animal culture SF1-1100 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/0ae8aa11dde74b20bcb8f0ab870b80ba
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spelling	oai:doaj.org-article:0ae8aa11dde74b20bcb8f0ab870b80ba2021-11-22T04:19:12ZAntimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment1056-617110.1016/j.japr.2021.100195https://doaj.org/article/0ae8aa11dde74b20bcb8f0ab870b80ba2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1056617121000581https://doaj.org/toc/1056-6171SUMMARY: Foodborne pathogens, such as Salmonella are problematic in food processing environments, and understanding the means of persistence is critical in the development of effective control measures. This study determined the antimicrobial tolerance of Salmonella isolates from the processing environment and characterized their biofilm production and antibiotic resistance. Twenty-five Salmonella isolates were previously recovered from poultry processing equipment in commercial production facilities after sanitation. The minimum inhibitory concentration of 2 antimicrobials; chlorine and quaternary ammonium compounds that were frequently used for sanitation was determined for these isolates using the Clinical and Laboratory Standards Institute guidelines. Biofilm forming ability was assessed using the crystal violet assay and antibiotic susceptibility was also determined. These isolates were further characterized based on their genes that were responsible for biofilm formation and resistance to sanitizers and antibiotics. Minimum inhibitory concentration values between 500 and 1,000 parts per million for chlorine, or 3 to 25 parts per million for quaternary ammonium compounds were observed amongst these Salmonella isolates. These isolates possessed strong (24%), moderate (28%), and weak (48%) biofilm forming ability. All isolates were resistant to multiple antibiotics, and 64% exhibited resistance to aminoglycosides and β-lactams. Molecular characterization showed that the isolates possessed specific genes for biofilm formation, sanitizer tolerance, and antibiotic resistance. These results suggest that Salmonella isolates with low tolerance to sanitizers may remain on surfaces because of their strong biofilm forming ability.T. ObeR. NannapaneniW. SchillingL. ZhangA. KiessElsevierarticleSalmonellabiofilmantibiotic resistancepoultry processingAnimal cultureSF1-1100Food processing and manufactureTP368-456ENJournal of Applied Poultry Research, Vol 30, Iss 4, Pp 100195- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella biofilm antibiotic resistance poultry processing Animal culture SF1-1100 Food processing and manufacture TP368-456
spellingShingle	Salmonella biofilm antibiotic resistance poultry processing Animal culture SF1-1100 Food processing and manufacture TP368-456 T. Obe R. Nannapaneni W. Schilling L. Zhang A. Kiess Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
description	SUMMARY: Foodborne pathogens, such as Salmonella are problematic in food processing environments, and understanding the means of persistence is critical in the development of effective control measures. This study determined the antimicrobial tolerance of Salmonella isolates from the processing environment and characterized their biofilm production and antibiotic resistance. Twenty-five Salmonella isolates were previously recovered from poultry processing equipment in commercial production facilities after sanitation. The minimum inhibitory concentration of 2 antimicrobials; chlorine and quaternary ammonium compounds that were frequently used for sanitation was determined for these isolates using the Clinical and Laboratory Standards Institute guidelines. Biofilm forming ability was assessed using the crystal violet assay and antibiotic susceptibility was also determined. These isolates were further characterized based on their genes that were responsible for biofilm formation and resistance to sanitizers and antibiotics. Minimum inhibitory concentration values between 500 and 1,000 parts per million for chlorine, or 3 to 25 parts per million for quaternary ammonium compounds were observed amongst these Salmonella isolates. These isolates possessed strong (24%), moderate (28%), and weak (48%) biofilm forming ability. All isolates were resistant to multiple antibiotics, and 64% exhibited resistance to aminoglycosides and β-lactams. Molecular characterization showed that the isolates possessed specific genes for biofilm formation, sanitizer tolerance, and antibiotic resistance. These results suggest that Salmonella isolates with low tolerance to sanitizers may remain on surfaces because of their strong biofilm forming ability.
format	article
author	T. Obe R. Nannapaneni W. Schilling L. Zhang A. Kiess
author_facet	T. Obe R. Nannapaneni W. Schilling L. Zhang A. Kiess
author_sort	T. Obe
title	Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
title_short	Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
title_full	Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
title_fullStr	Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
title_full_unstemmed	Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment
title_sort	antimicrobial tolerance, biofilm formation, and molecular characterization of salmonella isolates from poultry processing equipment
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0ae8aa11dde74b20bcb8f0ab870b80ba
work_keys_str_mv	AT tobe antimicrobialtolerancebiofilmformationandmolecularcharacterizationofsalmonellaisolatesfrompoultryprocessingequipment AT rnannapaneni antimicrobialtolerancebiofilmformationandmolecularcharacterizationofsalmonellaisolatesfrompoultryprocessingequipment AT wschilling antimicrobialtolerancebiofilmformationandmolecularcharacterizationofsalmonellaisolatesfrompoultryprocessingequipment AT lzhang antimicrobialtolerancebiofilmformationandmolecularcharacterizationofsalmonellaisolatesfrompoultryprocessingequipment AT akiess antimicrobialtolerancebiofilmformationandmolecularcharacterizationofsalmonellaisolatesfrompoultryprocessingequipment
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Antimicrobial tolerance, biofilm formation, and molecular characterization of Salmonella isolates from poultry processing equipment

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