The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer

Abstract Our previous studies revealed that oncogene CPNE1 is positively correlated with the occurrence, TNM stage, lymph node metastasis, and distant metastasis of non-small-cell lung cancer (NSCLC), and it could be regulated by micro RNAs. But no direct role of post-translational modification of C...

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Autores principales:	Ruochen Zhang, Weijie Zhang, Yuanyuan Zeng, Yue Li, Jieqi Zhou, Yang Zhang, Anqi Wang, Yantian Lv, Jianjie Zhu, Zeyi Liu, Jian-an Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/0aeaf8f4ff4a4bd987b772ee8b7f336f
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spelling	oai:doaj.org-article:0aeaf8f4ff4a4bd987b772ee8b7f336f2021-11-07T12:21:40ZThe regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer10.1038/s41420-021-00736-12058-7716https://doaj.org/article/0aeaf8f4ff4a4bd987b772ee8b7f336f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41420-021-00736-1https://doaj.org/toc/2058-7716Abstract Our previous studies revealed that oncogene CPNE1 is positively correlated with the occurrence, TNM stage, lymph node metastasis, and distant metastasis of non-small-cell lung cancer (NSCLC), and it could be regulated by micro RNAs. But no direct role of post-translational modification of CPNE1 in NSCLC has been reported. This study confirms that CPNE1 is degraded by two pathways: the ubiquitin-proteasome pathway and the autophagy-lysosome pathway. CPNE1 binds with the ubiquitin molecule via its K157 residue. Moreover, we determined that the ubiquitin ligase NEDD4L can mediate the ubiquitination of CPNE1 and promote its degradation. In addition, we find that NEDD4L knockdown promotes the proliferation and metastasis of NSCLC cells by regulating CPNE1 in vitro and vivo. This study aims to further investigate the mechanism of CPNE1 ubiquitination in the occurrence and development of NSCLC and provide a new potential target for NSCLC treatment.Ruochen ZhangWeijie ZhangYuanyuan ZengYue LiJieqi ZhouYang ZhangAnqi WangYantian LvJianjie ZhuZeyi LiuJian-an HuangNature Publishing GrouparticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCell Death Discovery, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Ruochen Zhang Weijie Zhang Yuanyuan Zeng Yue Li Jieqi Zhou Yang Zhang Anqi Wang Yantian Lv Jianjie Zhu Zeyi Liu Jian-an Huang The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
description	Abstract Our previous studies revealed that oncogene CPNE1 is positively correlated with the occurrence, TNM stage, lymph node metastasis, and distant metastasis of non-small-cell lung cancer (NSCLC), and it could be regulated by micro RNAs. But no direct role of post-translational modification of CPNE1 in NSCLC has been reported. This study confirms that CPNE1 is degraded by two pathways: the ubiquitin-proteasome pathway and the autophagy-lysosome pathway. CPNE1 binds with the ubiquitin molecule via its K157 residue. Moreover, we determined that the ubiquitin ligase NEDD4L can mediate the ubiquitination of CPNE1 and promote its degradation. In addition, we find that NEDD4L knockdown promotes the proliferation and metastasis of NSCLC cells by regulating CPNE1 in vitro and vivo. This study aims to further investigate the mechanism of CPNE1 ubiquitination in the occurrence and development of NSCLC and provide a new potential target for NSCLC treatment.
format	article
author	Ruochen Zhang Weijie Zhang Yuanyuan Zeng Yue Li Jieqi Zhou Yang Zhang Anqi Wang Yantian Lv Jianjie Zhu Zeyi Liu Jian-an Huang
author_facet	Ruochen Zhang Weijie Zhang Yuanyuan Zeng Yue Li Jieqi Zhou Yang Zhang Anqi Wang Yantian Lv Jianjie Zhu Zeyi Liu Jian-an Huang
author_sort	Ruochen Zhang
title	The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
title_short	The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
title_full	The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
title_fullStr	The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
title_full_unstemmed	The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer
title_sort	regulation of cpne1 ubiquitination by the nedd4l is involved in the pathogenesis of non-small cell lung cancer
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/0aeaf8f4ff4a4bd987b772ee8b7f336f
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The regulation of CPNE1 ubiquitination by the NEDD4L is involved in the pathogenesis of non-small cell lung cancer

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