Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming

Cold stress has become one of the main abiotic stresses in pepper, which severely limits the growth and development of pepper. In this study, the physiological indicators and transcriptome of a cold-tolerance (CT) inbred line A188 and a cold-sensitive (CS) inbred line A122 under cold–rewarm treatmen...

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Autores principales:	Wu Miao, Jingshuang Song, Yanwu Huang, Rongyun Liu, Gaofeng Zou, Lijun Ou, Zhoubin Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pepper cold stress transcriptome differentially expressed genes WGCNA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0aedf4f560e742d08624095af9d31b1e
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spelling	oai:doaj.org-article:0aedf4f560e742d08624095af9d31b1e2021-11-11T15:15:35ZComparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming10.3390/app1121102042076-3417https://doaj.org/article/0aedf4f560e742d08624095af9d31b1e2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10204https://doaj.org/toc/2076-3417Cold stress has become one of the main abiotic stresses in pepper, which severely limits the growth and development of pepper. In this study, the physiological indicators and transcriptome of a cold-tolerance (CT) inbred line A188 and a cold-sensitive (CS) inbred line A122 under cold–rewarm treatments were studied; the aim of this study was to determine the potential of the key factors in pepper response to cold stress. Compared with CT, CS wilts more seriously after cold stress, with poor resilience, higher content of malondialdehyde, and lower content of soluble sugar and total chlorophyll. Moreover, during cold treatment, 7333 and 5953 differentially expressed genes (DEGs) were observed for CT and CS, respectively. These DEGs were significantly enriched in pathways related to photosynthesis, plant hormone signal transduction, and DNA damage repair. Interestingly, in addition to the widely studied transcription factors related to cold, it was also found that 13 NAC transcription factors increased significantly in the T4 group; meanwhile, the <i>NAC8</i> (Capana02g003557) and <i>NAC72</i> (Capana07g002219) in CT were significantly higher than those in CS under rewarming for 1 h after 72 h cold treatment. Notably, weighted gene coexpression network analysis identified four positively correlated modules and eight hub genes, including zinc finger proteins, heat shock 70 kda protein, and cytochrome P450 family, which are related to cold tolerance. All of these pathways and genes may be responsible for the response to cold and even the cold tolerance in pepper.Wu MiaoJingshuang SongYanwu HuangRongyun LiuGaofeng ZouLijun OuZhoubin LiuMDPI AGarticlepeppercold stresstranscriptomedifferentially expressed genesWGCNATechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10204, p 10204 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pepper cold stress transcriptome differentially expressed genes WGCNA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	pepper cold stress transcriptome differentially expressed genes WGCNA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Wu Miao Jingshuang Song Yanwu Huang Rongyun Liu Gaofeng Zou Lijun Ou Zhoubin Liu Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
description	Cold stress has become one of the main abiotic stresses in pepper, which severely limits the growth and development of pepper. In this study, the physiological indicators and transcriptome of a cold-tolerance (CT) inbred line A188 and a cold-sensitive (CS) inbred line A122 under cold–rewarm treatments were studied; the aim of this study was to determine the potential of the key factors in pepper response to cold stress. Compared with CT, CS wilts more seriously after cold stress, with poor resilience, higher content of malondialdehyde, and lower content of soluble sugar and total chlorophyll. Moreover, during cold treatment, 7333 and 5953 differentially expressed genes (DEGs) were observed for CT and CS, respectively. These DEGs were significantly enriched in pathways related to photosynthesis, plant hormone signal transduction, and DNA damage repair. Interestingly, in addition to the widely studied transcription factors related to cold, it was also found that 13 NAC transcription factors increased significantly in the T4 group; meanwhile, the <i>NAC8</i> (Capana02g003557) and <i>NAC72</i> (Capana07g002219) in CT were significantly higher than those in CS under rewarming for 1 h after 72 h cold treatment. Notably, weighted gene coexpression network analysis identified four positively correlated modules and eight hub genes, including zinc finger proteins, heat shock 70 kda protein, and cytochrome P450 family, which are related to cold tolerance. All of these pathways and genes may be responsible for the response to cold and even the cold tolerance in pepper.
format	article
author	Wu Miao Jingshuang Song Yanwu Huang Rongyun Liu Gaofeng Zou Lijun Ou Zhoubin Liu
author_facet	Wu Miao Jingshuang Song Yanwu Huang Rongyun Liu Gaofeng Zou Lijun Ou Zhoubin Liu
author_sort	Wu Miao
title	Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
title_short	Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
title_full	Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
title_fullStr	Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
title_full_unstemmed	Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming
title_sort	comparative transcriptomics for pepper (<i>capsicum annuum</i> l.) under cold stress and after rewarming
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0aedf4f560e742d08624095af9d31b1e
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Comparative Transcriptomics for Pepper (<i>Capsicum annuum</i> L.) under Cold Stress and after Rewarming

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