Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro

Rui-Min Long,1,2,* Qing-Lei Dai,1,* Xia Zhou,1,* Duan-Hua Cai,1 Ya-Zhen Hong,1–3 Shi-Bin Wang,1–4 Yuan-Gang Liu1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao Universit...

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Autores principales:	Long R, Dai Q, Zhou X, Cai D, Hong Y, Wang SB, Liu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Bacterial magnetosomes siRNA Doxorubicin Combination therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0af09bd456ab43fda8b54b112261168c
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spelling	oai:doaj.org-article:0af09bd456ab43fda8b54b112261168c2021-12-02T04:58:31ZBacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro1178-2013https://doaj.org/article/0af09bd456ab43fda8b54b112261168c2018-12-01T00:00:00Zhttps://www.dovepress.com/bacterial-magnetosomes-based-nanocarriers-for-co-delivery-of-cancer-th-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rui-Min Long,1,2,* Qing-Lei Dai,1,* Xia Zhou,1,* Duan-Hua Cai,1 Ya-Zhen Hong,1–3 Shi-Bin Wang,1–4 Yuan-Gang Liu1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 3Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China; 4Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China *These authors contributed equally to this work Abstract: In recent times, co-delivery of therapeutics has emerged as a promising strategy for treating dreadful diseases such as cancer.Materials and methods: In this study, we developed a novel nanocarrier based on bacterial magnetosomes (BMs) that co-loaded with siRNA and doxorubicin (DOX) using polyethyleneimine (PEI) as a cross-linker (BMs/DP/siRNA). The delivery efficiency of siRNA as well as the pH-responsive release of DOX, and synergistic efficacy of these therapeutics in vitro were systematically investigated.Results: The structure of DOX–PEI (DP) conjugates that synthesized via hydrazone bond formation was confirmed by 1H nuclear magnetic resonance (NMR). The in vitro release experiments showed that the DP conjugate (DOX-loading efficiency – 5.77%±0.08%) exhibited the long-term release behavior. Furthermore, the optimal BMs/DP/siRNA particle size of 107.2 nm and the zeta potential value of 31.1±1.0 mV facilitated enhanced cellular internalization efficiency. Moreover, the agarose gel electrophoresis showed that the co-delivery system could protect siRNA from degradation in serum and RNase A. In addition, the cytotoxicity assay showed that BMs/DP/siRNA could achieve an excellent synergistic effect compared to that of siRNA delivery alone. The acridine orange (AO)/ethidium bromide (EB) double staining assay also showed that BMs/DP/siRNA complex could induce cells in a stage of late apoptosis and nanocomplex located in the proximity of the nucleus.Conclusion: The combination of gene and chemotherapeutic drug using BMs is highly efficient, and the BMs/DP/siRNA would be a promising therapeutic strategy for the future therapeutics. Keywords: bacterial magnetosomes, co-delivery, gene therapy, pH-responsive release Long RDai QZhou XCai DHong YWang SBLiu YDove Medical PressarticleBacterial magnetosomessiRNADoxorubicinCombination therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 8269-8279 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacterial magnetosomes siRNA Doxorubicin Combination therapy Medicine (General) R5-920
spellingShingle	Bacterial magnetosomes siRNA Doxorubicin Combination therapy Medicine (General) R5-920 Long R Dai Q Zhou X Cai D Hong Y Wang SB Liu Y Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
description	Rui-Min Long,1,2,* Qing-Lei Dai,1,* Xia Zhou,1,* Duan-Hua Cai,1 Ya-Zhen Hong,1–3 Shi-Bin Wang,1–4 Yuan-Gang Liu1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 3Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China; 4Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China *These authors contributed equally to this work Abstract: In recent times, co-delivery of therapeutics has emerged as a promising strategy for treating dreadful diseases such as cancer.Materials and methods: In this study, we developed a novel nanocarrier based on bacterial magnetosomes (BMs) that co-loaded with siRNA and doxorubicin (DOX) using polyethyleneimine (PEI) as a cross-linker (BMs/DP/siRNA). The delivery efficiency of siRNA as well as the pH-responsive release of DOX, and synergistic efficacy of these therapeutics in vitro were systematically investigated.Results: The structure of DOX–PEI (DP) conjugates that synthesized via hydrazone bond formation was confirmed by 1H nuclear magnetic resonance (NMR). The in vitro release experiments showed that the DP conjugate (DOX-loading efficiency – 5.77%±0.08%) exhibited the long-term release behavior. Furthermore, the optimal BMs/DP/siRNA particle size of 107.2 nm and the zeta potential value of 31.1±1.0 mV facilitated enhanced cellular internalization efficiency. Moreover, the agarose gel electrophoresis showed that the co-delivery system could protect siRNA from degradation in serum and RNase A. In addition, the cytotoxicity assay showed that BMs/DP/siRNA could achieve an excellent synergistic effect compared to that of siRNA delivery alone. The acridine orange (AO)/ethidium bromide (EB) double staining assay also showed that BMs/DP/siRNA complex could induce cells in a stage of late apoptosis and nanocomplex located in the proximity of the nucleus.Conclusion: The combination of gene and chemotherapeutic drug using BMs is highly efficient, and the BMs/DP/siRNA would be a promising therapeutic strategy for the future therapeutics. Keywords: bacterial magnetosomes, co-delivery, gene therapy, pH-responsive release
format	article
author	Long R Dai Q Zhou X Cai D Hong Y Wang SB Liu Y
author_facet	Long R Dai Q Zhou X Cai D Hong Y Wang SB Liu Y
author_sort	Long R
title	Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
title_short	Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
title_full	Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
title_fullStr	Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
title_full_unstemmed	Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
title_sort	bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/0af09bd456ab43fda8b54b112261168c
work_keys_str_mv	AT longr bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT daiq bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT zhoux bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT caid bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT hongy bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT wangsb bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro AT liuy bacterialmagnetosomesbasednanocarriersforcodeliveryofcancertherapeuticsinvitro
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Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro

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