Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model

Abstract Tonsil-derived mesenchymal stem cells (TMSCs) showed therapeutic effects on acute and chronic murine colitis models, owing to their immunomodulatory properties; therefore, we evaluated enhanced therapeutic effects of TMSCs on a murine colitis model using three-dimensional (3D) culture metho...

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Autores principales:	Eun Mi Song, Yang Hee Joo, A. Reum Choe, Yehyun Park, Chung Hyun Tae, Ji Teak Hong, Chang Mo Moon, Seong-Eun Kim, Hye-Kyung Jung, Ki-Nam Shim, Kyung-Ah Cho, Inho Jo, Sung-Ae Jung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b001ad955914f3eb30cba980ed76691
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spelling	oai:doaj.org-article:0b001ad955914f3eb30cba980ed766912021-12-02T17:17:38ZThree-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model10.1038/s41598-021-98711-42045-2322https://doaj.org/article/0b001ad955914f3eb30cba980ed766912021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98711-4https://doaj.org/toc/2045-2322Abstract Tonsil-derived mesenchymal stem cells (TMSCs) showed therapeutic effects on acute and chronic murine colitis models, owing to their immunomodulatory properties; therefore, we evaluated enhanced therapeutic effects of TMSCs on a murine colitis model using three-dimensional (3D) culture method. The expression of angiogenic factors, VEGF, and anti-inflammatory cytokines, IL-10, TSG-6, TGF-β, and IDO-1, was significantly higher in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P < 0.05). At days 18 and 30 after inducing chronic colitis, disease activity index scores were estimated to be significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001 and P < 0.001, respectively) and 2D-TMSC-treated groups (P = 0.022 and P = 0.004, respectively). Body weight loss was significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001) and 2D-TMSC-treated groups (P = 0.005). Colon length shortening was significantly recovered in the 3D-TMSC-treated group compared to that in the 2D-TMSC-treated group (P = 0.001). Histological scoring index was significantly lower in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P = 0.002). These results indicate that 3D-cultured TMSCs showed considerably higher therapeutic effects in a chronic murine colitis model than those of 2D-cultured TMSCs via increased anti-inflammatory cytokine expression.Eun Mi SongYang Hee JooA. Reum ChoeYehyun ParkChung Hyun TaeJi Teak HongChang Mo MoonSeong-Eun KimHye-Kyung JungKi-Nam ShimKyung-Ah ChoInho JoSung-Ae JungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun Mi Song Yang Hee Joo A. Reum Choe Yehyun Park Chung Hyun Tae Ji Teak Hong Chang Mo Moon Seong-Eun Kim Hye-Kyung Jung Ki-Nam Shim Kyung-Ah Cho Inho Jo Sung-Ae Jung Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
description	Abstract Tonsil-derived mesenchymal stem cells (TMSCs) showed therapeutic effects on acute and chronic murine colitis models, owing to their immunomodulatory properties; therefore, we evaluated enhanced therapeutic effects of TMSCs on a murine colitis model using three-dimensional (3D) culture method. The expression of angiogenic factors, VEGF, and anti-inflammatory cytokines, IL-10, TSG-6, TGF-β, and IDO-1, was significantly higher in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P < 0.05). At days 18 and 30 after inducing chronic colitis, disease activity index scores were estimated to be significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001 and P < 0.001, respectively) and 2D-TMSC-treated groups (P = 0.022 and P = 0.004, respectively). Body weight loss was significantly lower in the 3D-TMSC-treated group than in the colitis control (P < 0.001) and 2D-TMSC-treated groups (P = 0.005). Colon length shortening was significantly recovered in the 3D-TMSC-treated group compared to that in the 2D-TMSC-treated group (P = 0.001). Histological scoring index was significantly lower in the 3D-TMSC-treated group than in the 2D-TMSC-treated group (P = 0.002). These results indicate that 3D-cultured TMSCs showed considerably higher therapeutic effects in a chronic murine colitis model than those of 2D-cultured TMSCs via increased anti-inflammatory cytokine expression.
format	article
author	Eun Mi Song Yang Hee Joo A. Reum Choe Yehyun Park Chung Hyun Tae Ji Teak Hong Chang Mo Moon Seong-Eun Kim Hye-Kyung Jung Ki-Nam Shim Kyung-Ah Cho Inho Jo Sung-Ae Jung
author_facet	Eun Mi Song Yang Hee Joo A. Reum Choe Yehyun Park Chung Hyun Tae Ji Teak Hong Chang Mo Moon Seong-Eun Kim Hye-Kyung Jung Ki-Nam Shim Kyung-Ah Cho Inho Jo Sung-Ae Jung
author_sort	Eun Mi Song
title	Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
title_short	Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
title_full	Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
title_fullStr	Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
title_full_unstemmed	Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
title_sort	three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0b001ad955914f3eb30cba980ed76691
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Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model

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