INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI

Abstract. The article concerns interactions between immunoglobulin A and recombinant P6, P7, P8 polypeptides, designed on the basis of externally localized Bac protein of the Group B streptococci, possessing IgA-binding activity.There is a current demand for immunochemical reagents that are strictly...

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Autores principales:	A. S. Korzhueva, D. G. Ibragimova, I. A. Ustinovich, Artem A. Totolian, M. P. Samoilovich, A. N. Suvorov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	recombinant polypeptides group b streptococci iga receptor вас protein immunochemical reagents Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0b119999c51a493a92ed3c13f11d1f9f
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spelling	oai:doaj.org-article:0b119999c51a493a92ed3c13f11d1f9f2021-11-18T08:03:39ZINTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI1563-06252313-741X10.15789/1563-0625-2008-4-5-327-336https://doaj.org/article/0b119999c51a493a92ed3c13f11d1f9f2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/182https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The article concerns interactions between immunoglobulin A and recombinant P6, P7, P8 polypeptides, designed on the basis of externally localized Bac protein of the Group B streptococci, possessing IgA-binding activity.There is a current demand for immunochemical reagents that are strictly specific for IgA, in order to develop antigenic standards for detection of IgA levels in biological fluids, as well as for affinity purification of IgA and its fragments.To analyze an opportunity of the abovementioned application ways for these proteins, a special study was performed to assay an interaction capability of recombinant P6, P7, P8 polypeptides binding to Fc regions of different IgA forms (serum IgA, secretory IgA, subclasses of serum IgA – IgA1, IgA2). Selectivity of ligand binding was specially confirmed.It was found out that, among three presented polypeptides, the structure of recombinant P6 derivative proved to be optimal for IgA-binding ability of Bac protein.Structural features of IgA-binding fragments of Bac protein, i.e., binding site position on the IgA molecule (proximity to epitopes for three monoclonal antibodies), variability of the site structure, as well as resistance of binding site for P6, P7, P8 in IgA molecule against partial disulfide bonds reduction. (Med. Immunol., vol. 10, N 4-5, pp 327-336).A. S. KorzhuevaD. G. IbragimovaI. A. UstinovichArtem A. TotolianM. P. SamoilovichA. N. SuvorovSPb RAACIarticlerecombinant polypeptidesgroup b streptococciiga receptorвас proteinimmunochemical reagentsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 4-5, Pp 327-336 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	recombinant polypeptides group b streptococci iga receptor вас protein immunochemical reagents Immunologic diseases. Allergy RC581-607
spellingShingle	recombinant polypeptides group b streptococci iga receptor вас protein immunochemical reagents Immunologic diseases. Allergy RC581-607 A. S. Korzhueva D. G. Ibragimova I. A. Ustinovich Artem A. Totolian M. P. Samoilovich A. N. Suvorov INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
description	Abstract. The article concerns interactions between immunoglobulin A and recombinant P6, P7, P8 polypeptides, designed on the basis of externally localized Bac protein of the Group B streptococci, possessing IgA-binding activity.There is a current demand for immunochemical reagents that are strictly specific for IgA, in order to develop antigenic standards for detection of IgA levels in biological fluids, as well as for affinity purification of IgA and its fragments.To analyze an opportunity of the abovementioned application ways for these proteins, a special study was performed to assay an interaction capability of recombinant P6, P7, P8 polypeptides binding to Fc regions of different IgA forms (serum IgA, secretory IgA, subclasses of serum IgA – IgA1, IgA2). Selectivity of ligand binding was specially confirmed.It was found out that, among three presented polypeptides, the structure of recombinant P6 derivative proved to be optimal for IgA-binding ability of Bac protein.Structural features of IgA-binding fragments of Bac protein, i.e., binding site position on the IgA molecule (proximity to epitopes for three monoclonal antibodies), variability of the site structure, as well as resistance of binding site for P6, P7, P8 in IgA molecule against partial disulfide bonds reduction. (Med. Immunol., vol. 10, N 4-5, pp 327-336).
format	article
author	A. S. Korzhueva D. G. Ibragimova I. A. Ustinovich Artem A. Totolian M. P. Samoilovich A. N. Suvorov
author_facet	A. S. Korzhueva D. G. Ibragimova I. A. Ustinovich Artem A. Totolian M. P. Samoilovich A. N. Suvorov
author_sort	A. S. Korzhueva
title	INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
title_short	INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
title_full	INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
title_fullStr	INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
title_full_unstemmed	INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI
title_sort	interaction between different molecular forms of immunoglobulin a and recombinant derivatives polypeptides of bac receptor proteins from group b streptococci
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/0b119999c51a493a92ed3c13f11d1f9f
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_version_	1718422523460190208

INTERACTION BETWEEN DIFFERENT MOLECULAR FORMS OF IMMUNOGLOBULIN A AND RECOMBINANT DERIVATIVES POLYPEPTIDES OF BAC RECEPTOR PROTEINS FROM GROUP B STREPTOCOCCI

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