Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran

Background: Helicobacter pullorum predominantly colonizes the gut of apparently healthy chickens and the livers and intestinal contents of hens with enteritis and vibrionic hepatitis. Objective: The aim of this study was to assess the prevalence and antibiotic resistance of Helicobacter pullorum in...

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Autores principales:	Hosein Akhlaghi, Seyed Hesamodin Emadi Chashmi, Ashkan Jebelli Javan
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	helicobacter pullorum poultry culture method polymerase chain reaction antibiotic resistance Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/0b12d4f931e44147859368844bf7818f
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spelling	oai:doaj.org-article:0b12d4f931e44147859368844bf7818f2021-11-18T06:56:46ZPrevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran2345-33622322-586610.34172/ijep.2020.22https://doaj.org/article/0b12d4f931e44147859368844bf7818f2020-08-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4366https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Helicobacter pullorum predominantly colonizes the gut of apparently healthy chickens and the livers and intestinal contents of hens with enteritis and vibrionic hepatitis. Objective: The aim of this study was to assess the prevalence and antibiotic resistance of Helicobacter pullorum in broiler chickens, laying hens, and turkeys in Semnan province. Materials and Methods: A total of 300 samples were collected from 60 poultry farms in Semnan province, including 240 cecal samples from 48 broiler farms, 30 fecal samples from 6 laying hen farms, and 30 cecal samples from 6 turkey farms. Each sample was analyzed by conventional culture method and biochemical tests. The suspected colonies were subjected to polymerase chain reaction (PCR) using 16S rRNA gene. Antibiotic resistance of the confirmed colonies was determined using disk diffusion method. Results: Of 300 samples, 85 (28.3%) samples obtained from 36 (60%) poultry farms were positive for H. pullorum. Of these samples, 72 (30%) were from 30 (62.5%) broiler farms, 4 (13.3%) were from 2 (33.3%) laying hen farms, and 9 (30%) were from 4 (66.7%) turkey farms. Moreover, resistance to ciprofloxacin was observed in all of the H. pullorum isolates. Conclusion: This study demonstrated the moderate prevalence of H. pullorum in poultry in Semnan province for the first time, while the prevalence of this pathogen in laying hen and turkey has not been determined in Iran. In addition, this study could reveal the antibiotic resistance profile of H. pullorum as the first report in Iran. Therefore, more studies are needed to focus on the prevalence and antibiotic resistance of H. pullorum in poultry in other regions of Iran.Hosein AkhlaghiSeyed Hesamodin Emadi ChashmiAshkan Jebelli JavanAlborz University of Medical Sciencesarticlehelicobacter pullorum poultry culture method polymerase chain reaction antibiotic resistance Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 3, Pp 101-106 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	helicobacter pullorum poultry culture method polymerase chain reaction antibiotic resistance Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	helicobacter pullorum poultry culture method polymerase chain reaction antibiotic resistance Diseases of the digestive system. Gastroenterology RC799-869 Hosein Akhlaghi Seyed Hesamodin Emadi Chashmi Ashkan Jebelli Javan Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
description	Background: Helicobacter pullorum predominantly colonizes the gut of apparently healthy chickens and the livers and intestinal contents of hens with enteritis and vibrionic hepatitis. Objective: The aim of this study was to assess the prevalence and antibiotic resistance of Helicobacter pullorum in broiler chickens, laying hens, and turkeys in Semnan province. Materials and Methods: A total of 300 samples were collected from 60 poultry farms in Semnan province, including 240 cecal samples from 48 broiler farms, 30 fecal samples from 6 laying hen farms, and 30 cecal samples from 6 turkey farms. Each sample was analyzed by conventional culture method and biochemical tests. The suspected colonies were subjected to polymerase chain reaction (PCR) using 16S rRNA gene. Antibiotic resistance of the confirmed colonies was determined using disk diffusion method. Results: Of 300 samples, 85 (28.3%) samples obtained from 36 (60%) poultry farms were positive for H. pullorum. Of these samples, 72 (30%) were from 30 (62.5%) broiler farms, 4 (13.3%) were from 2 (33.3%) laying hen farms, and 9 (30%) were from 4 (66.7%) turkey farms. Moreover, resistance to ciprofloxacin was observed in all of the H. pullorum isolates. Conclusion: This study demonstrated the moderate prevalence of H. pullorum in poultry in Semnan province for the first time, while the prevalence of this pathogen in laying hen and turkey has not been determined in Iran. In addition, this study could reveal the antibiotic resistance profile of H. pullorum as the first report in Iran. Therefore, more studies are needed to focus on the prevalence and antibiotic resistance of H. pullorum in poultry in other regions of Iran.
format	article
author	Hosein Akhlaghi Seyed Hesamodin Emadi Chashmi Ashkan Jebelli Javan
author_facet	Hosein Akhlaghi Seyed Hesamodin Emadi Chashmi Ashkan Jebelli Javan
author_sort	Hosein Akhlaghi
title	Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
title_short	Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
title_full	Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
title_fullStr	Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
title_full_unstemmed	Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran
title_sort	prevalence and antibiotic resistance of helicobacter pullorum isolates in poultry from semnan province, iran
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/0b12d4f931e44147859368844bf7818f
work_keys_str_mv	AT hoseinakhlaghi prevalenceandantibioticresistanceofhelicobacterpullorumisolatesinpoultryfromsemnanprovinceiran AT seyedhesamodinemadichashmi prevalenceandantibioticresistanceofhelicobacterpullorumisolatesinpoultryfromsemnanprovinceiran AT ashkanjebellijavan prevalenceandantibioticresistanceofhelicobacterpullorumisolatesinpoultryfromsemnanprovinceiran
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Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran

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