BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes

White adipocytes contribute to energy storage, accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation i...

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Autores principales:	Abhirup Shaw, Beáta B. Tóth, Rini Arianti, István Csomós, Szilárd Póliska, Attila Vámos, Zsolt Bacso, Ferenc Győry, László Fésüs, Endre Kristóf
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	BMP7 adipocyte UCP1 thermogenesis creatine cycle obesity Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/0b19711fcc0848debcece1e19d63770b
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spelling	oai:doaj.org-article:0b19711fcc0848debcece1e19d63770b2021-11-25T18:39:09ZBMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes10.3390/ph141110781424-8247https://doaj.org/article/0b19711fcc0848debcece1e19d63770b2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1078https://doaj.org/toc/1424-8247White adipocytes contribute to energy storage, accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation in murine interscapular adipose tissue. Here, we performed global RNA-sequencing and functional assays on adipocytes obtained from subcutaneous (SC) and deep-neck (DN) depots of human neck and differentiated with or without BMP7. We found that BMP7 did not influence differentiation but upregulated browning markers, including UCP1 mRNA and protein in SC and DN derived adipocytes. BMP7 also enhanced mitochondrial DNA content, levels of oxidative phosphorylation complex subunits, along with PGC1α and p-CREB upregulation, and fragmentation of mitochondria. Furthermore, both UCP1-dependent proton leak and UCP1-independent, creatine-driven substrate cycle coupled thermogenesis were augmented upon BMP7 addition. The gene expression analysis also shed light on the possible role of genes unrelated to thermogenesis thus far, including ACAN, CRYAB, and ID1, which were among the highest upregulated ones by BMP7 treatment in both types of adipocytes. Together, our study shows that BMP7 strongly upregulates thermogenesis in human neck area derived adipocytes, along with genes, which might have a supporting role in energy expenditure.Abhirup ShawBeáta B. TóthRini AriantiIstván CsomósSzilárd PóliskaAttila VámosZsolt BacsoFerenc GyőryLászló FésüsEndre KristófMDPI AGarticleBMP7adipocyteUCP1thermogenesiscreatine cycleobesityMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1078, p 1078 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BMP7 adipocyte UCP1 thermogenesis creatine cycle obesity Medicine R Pharmacy and materia medica RS1-441
spellingShingle	BMP7 adipocyte UCP1 thermogenesis creatine cycle obesity Medicine R Pharmacy and materia medica RS1-441 Abhirup Shaw Beáta B. Tóth Rini Arianti István Csomós Szilárd Póliska Attila Vámos Zsolt Bacso Ferenc Győry László Fésüs Endre Kristóf BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
description	White adipocytes contribute to energy storage, accumulating lipid droplets, whereas brown and beige adipocytes mainly function in dissipating energy as heat primarily via the action of uncoupling protein 1 (UCP1). Bone morphogenic protein 7 (BMP7) was shown to drive brown adipocyte differentiation in murine interscapular adipose tissue. Here, we performed global RNA-sequencing and functional assays on adipocytes obtained from subcutaneous (SC) and deep-neck (DN) depots of human neck and differentiated with or without BMP7. We found that BMP7 did not influence differentiation but upregulated browning markers, including UCP1 mRNA and protein in SC and DN derived adipocytes. BMP7 also enhanced mitochondrial DNA content, levels of oxidative phosphorylation complex subunits, along with PGC1α and p-CREB upregulation, and fragmentation of mitochondria. Furthermore, both UCP1-dependent proton leak and UCP1-independent, creatine-driven substrate cycle coupled thermogenesis were augmented upon BMP7 addition. The gene expression analysis also shed light on the possible role of genes unrelated to thermogenesis thus far, including ACAN, CRYAB, and ID1, which were among the highest upregulated ones by BMP7 treatment in both types of adipocytes. Together, our study shows that BMP7 strongly upregulates thermogenesis in human neck area derived adipocytes, along with genes, which might have a supporting role in energy expenditure.
format	article
author	Abhirup Shaw Beáta B. Tóth Rini Arianti István Csomós Szilárd Póliska Attila Vámos Zsolt Bacso Ferenc Győry László Fésüs Endre Kristóf
author_facet	Abhirup Shaw Beáta B. Tóth Rini Arianti István Csomós Szilárd Póliska Attila Vámos Zsolt Bacso Ferenc Győry László Fésüs Endre Kristóf
author_sort	Abhirup Shaw
title	BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
title_short	BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
title_full	BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
title_fullStr	BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
title_full_unstemmed	BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes
title_sort	bmp7 increases ucp1-dependent and independent thermogenesis with a unique gene expression program in human neck area derived adipocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0b19711fcc0848debcece1e19d63770b
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BMP7 Increases UCP1-Dependent and Independent Thermogenesis with a Unique Gene Expression Program in Human Neck Area Derived Adipocytes

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