Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation

Abdulrahman E Koshak,1 Mardi M Algandaby,2,3 Mohammad I Mujallid,3 Ashraf B Abdel-Naim,4 Nabil A Alhakamy,5 Usama A Fahmy,5 Anas Alfarsi,5 Shaimaa M Badr-Eldin,5,6 Thikryat Neamatallah,4 Mohammed Z Nasrullah,4 Hossam M Abdallah,1,7 Ahmed Esmat8,9 1Department of Natural Products and Alternative Medic...

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Autores principales:	Koshak AE, Algandaby MM, Mujallid MI, Abdel-Naim AB, Alhakamy NA, Fahmy UA, Alfarsi A, Badr-Eldin SM, Neamatallah T, Nasrullah MZ, Abdallah HM, Esmat A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	opuntia ficus-indica snedds wound healing tgf-β vegf rats Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0b1d705a5e3f44f4b0f383063b9e684b
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spelling	oai:doaj.org-article:0b1d705a5e3f44f4b0f383063b9e684b2021-12-02T14:34:43ZWound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation1178-2013https://doaj.org/article/0b1d705a5e3f44f4b0f383063b9e684b2021-06-01T00:00:00Zhttps://www.dovepress.com/wound-healing-activity-of-opuntia-ficus-indica-fixed-oil-formulated-in-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Abdulrahman E Koshak,1 Mardi M Algandaby,2,3 Mohammad I Mujallid,3 Ashraf B Abdel-Naim,4 Nabil A Alhakamy,5 Usama A Fahmy,5 Anas Alfarsi,5 Shaimaa M Badr-Eldin,5,6 Thikryat Neamatallah,4 Mohammed Z Nasrullah,4 Hossam M Abdallah,1,7 Ahmed Esmat8,9 1Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 2Medicinal Plants Research Group, Deanship of Scientific Research, King Abdulaziz University, Jeddah, Saudi Arabia; 3Faculty of Science, Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 5Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 7Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 8Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 9Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptCorrespondence: Hossam M AbdallahDepartment of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaTel +966544733110Email hmafifi@kau.edu.saIntroduction: Delayed wound healing represents a common health hazard. Traditional herbal products have been often utilized to promote wound contraction. The current study aimed at assessing the wound healing activity of Opuntia ficus-indica seed oil (OFI) and its self-nanoemulsifying drug delivery system (OFI-SNEDDS) formula in a rat model of full-thickness skin excision.Methods: Based on droplet size, an optimized OFI-SNEDDS formula was prepared and used for subsequent evaluation. Wound healing activity of OFI and OFI-SNEDDS was studied in vivo.Results: The optimized OFI-SNEDDS formula droplet size was 50.02 nm. The formula exhibited superior healing activities as compared to regular OFI seed oil-treated rats at day 14 of wounding. This effect was further confirmed by histopathological examinations of H&E and Masson’s Trichrome-stained skin sections. Moreover, OFI-SNEDDS showed the highest antioxidant and anti-inflammatory activities as compared to OFI seed oil-treated animals. Both OFI and OFI-SNEDDS significantly enhanced hydroxyproline skin content and upregulated Col1A1 mRNA expression, accompanied by enhanced expression of transforming factor-beta (TGF-β). Further, OFI-SNEDDS improved angiogenesis as evidenced by increased expression of vascular endothelial growth factor (VEGF).Conclusion: OFI possesses wound healing properties that are enhanced by self-emulsification of the oil into nano-droplets. The observed activity can be attributed, at least partly, to its anti-inflammatory, pro-collagen and angiogenic properties.Keywords: Opuntia ficus-indica, SNEDDS, wound healing, TGF-β, VEGF, ratsKoshak AEAlgandaby MMMujallid MIAbdel-Naim ABAlhakamy NAFahmy UAAlfarsi ABadr-Eldin SMNeamatallah TNasrullah MZAbdallah HMEsmat ADove Medical Pressarticleopuntia ficus-indicasneddswound healingtgf-βvegfratsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3889-3905 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	opuntia ficus-indica snedds wound healing tgf-β vegf rats Medicine (General) R5-920
spellingShingle	opuntia ficus-indica snedds wound healing tgf-β vegf rats Medicine (General) R5-920 Koshak AE Algandaby MM Mujallid MI Abdel-Naim AB Alhakamy NA Fahmy UA Alfarsi A Badr-Eldin SM Neamatallah T Nasrullah MZ Abdallah HM Esmat A Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
description	Abdulrahman E Koshak,1 Mardi M Algandaby,2,3 Mohammad I Mujallid,3 Ashraf B Abdel-Naim,4 Nabil A Alhakamy,5 Usama A Fahmy,5 Anas Alfarsi,5 Shaimaa M Badr-Eldin,5,6 Thikryat Neamatallah,4 Mohammed Z Nasrullah,4 Hossam M Abdallah,1,7 Ahmed Esmat8,9 1Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 2Medicinal Plants Research Group, Deanship of Scientific Research, King Abdulaziz University, Jeddah, Saudi Arabia; 3Faculty of Science, Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 5Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 7Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 8Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 9Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptCorrespondence: Hossam M AbdallahDepartment of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaTel +966544733110Email hmafifi@kau.edu.saIntroduction: Delayed wound healing represents a common health hazard. Traditional herbal products have been often utilized to promote wound contraction. The current study aimed at assessing the wound healing activity of Opuntia ficus-indica seed oil (OFI) and its self-nanoemulsifying drug delivery system (OFI-SNEDDS) formula in a rat model of full-thickness skin excision.Methods: Based on droplet size, an optimized OFI-SNEDDS formula was prepared and used for subsequent evaluation. Wound healing activity of OFI and OFI-SNEDDS was studied in vivo.Results: The optimized OFI-SNEDDS formula droplet size was 50.02 nm. The formula exhibited superior healing activities as compared to regular OFI seed oil-treated rats at day 14 of wounding. This effect was further confirmed by histopathological examinations of H&E and Masson’s Trichrome-stained skin sections. Moreover, OFI-SNEDDS showed the highest antioxidant and anti-inflammatory activities as compared to OFI seed oil-treated animals. Both OFI and OFI-SNEDDS significantly enhanced hydroxyproline skin content and upregulated Col1A1 mRNA expression, accompanied by enhanced expression of transforming factor-beta (TGF-β). Further, OFI-SNEDDS improved angiogenesis as evidenced by increased expression of vascular endothelial growth factor (VEGF).Conclusion: OFI possesses wound healing properties that are enhanced by self-emulsification of the oil into nano-droplets. The observed activity can be attributed, at least partly, to its anti-inflammatory, pro-collagen and angiogenic properties.Keywords: Opuntia ficus-indica, SNEDDS, wound healing, TGF-β, VEGF, rats
format	article
author	Koshak AE Algandaby MM Mujallid MI Abdel-Naim AB Alhakamy NA Fahmy UA Alfarsi A Badr-Eldin SM Neamatallah T Nasrullah MZ Abdallah HM Esmat A
author_facet	Koshak AE Algandaby MM Mujallid MI Abdel-Naim AB Alhakamy NA Fahmy UA Alfarsi A Badr-Eldin SM Neamatallah T Nasrullah MZ Abdallah HM Esmat A
author_sort	Koshak AE
title	Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
title_short	Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
title_full	Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
title_fullStr	Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
title_full_unstemmed	Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation
title_sort	wound healing activity of opuntia ficus-indica fixed oil formulated in a self-nanoemulsifying formulation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/0b1d705a5e3f44f4b0f383063b9e684b
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Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying Formulation

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