IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3

Abstract IgG3, passively administered together with small proteins, induces enhanced primary humoral responses against these proteins. We previously found that, within 2 h of immunization, marginal zone (MZ) B cells capture IgG3-antigen complexes and transport them into splenic follicles and that th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lu Zhang, Zhoujie Ding, Birgitta Heyman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b1f104a55e149b697351d1a4462ef65
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0b1f104a55e149b697351d1a4462ef65
record_format	dspace
spelling	oai:doaj.org-article:0b1f104a55e149b697351d1a4462ef652021-12-02T11:52:28ZIgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C310.1038/s41598-017-05704-32045-2322https://doaj.org/article/0b1f104a55e149b697351d1a4462ef652017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05704-3https://doaj.org/toc/2045-2322Abstract IgG3, passively administered together with small proteins, induces enhanced primary humoral responses against these proteins. We previously found that, within 2 h of immunization, marginal zone (MZ) B cells capture IgG3-antigen complexes and transport them into splenic follicles and that this requires the presence of complement receptors 1 and 2. We have here investigated the localization of IgG3 anti-2, 4, 6-trinitrophenyl (TNP)/biotin-ovalbumin-TNP immune complexes in the follicles and the involvement of classical versus total complement activation in this process. The majority (50–90%) of antigen inside the follicles of mice immunized with IgG3-antigen complexes co-localized with the follicular dendritic cell (FDC) network. Capture of antigen by MZ B cells as well as antigen deposition on FDC was severely impaired in mice lacking C1q or C3, and lack of either C1q or C3 also impaired the ability of IgG3 to enhance antibody responses. Finally, IgG3 efficiently primed for a memory response against small proteins as well as against the large protein keyhole limpet hemocyanine.Lu ZhangZhoujie DingBirgitta HeymanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lu Zhang Zhoujie Ding Birgitta Heyman IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
description	Abstract IgG3, passively administered together with small proteins, induces enhanced primary humoral responses against these proteins. We previously found that, within 2 h of immunization, marginal zone (MZ) B cells capture IgG3-antigen complexes and transport them into splenic follicles and that this requires the presence of complement receptors 1 and 2. We have here investigated the localization of IgG3 anti-2, 4, 6-trinitrophenyl (TNP)/biotin-ovalbumin-TNP immune complexes in the follicles and the involvement of classical versus total complement activation in this process. The majority (50–90%) of antigen inside the follicles of mice immunized with IgG3-antigen complexes co-localized with the follicular dendritic cell (FDC) network. Capture of antigen by MZ B cells as well as antigen deposition on FDC was severely impaired in mice lacking C1q or C3, and lack of either C1q or C3 also impaired the ability of IgG3 to enhance antibody responses. Finally, IgG3 efficiently primed for a memory response against small proteins as well as against the large protein keyhole limpet hemocyanine.
format	article
author	Lu Zhang Zhoujie Ding Birgitta Heyman
author_facet	Lu Zhang Zhoujie Ding Birgitta Heyman
author_sort	Lu Zhang
title	IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
title_short	IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
title_full	IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
title_fullStr	IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
title_full_unstemmed	IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3
title_sort	igg3-antigen complexes are deposited on follicular dendritic cells in the presence of c1q and c3
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0b1f104a55e149b697351d1a4462ef65
work_keys_str_mv	AT luzhang igg3antigencomplexesaredepositedonfolliculardendriticcellsinthepresenceofc1qandc3 AT zhoujieding igg3antigencomplexesaredepositedonfolliculardendriticcellsinthepresenceofc1qandc3 AT birgittaheyman igg3antigencomplexesaredepositedonfolliculardendriticcellsinthepresenceofc1qandc3
_version_	1718395029116944384

IgG3-antigen complexes are deposited on follicular dendritic cells in the presence of C1q and C3

Ejemplares similares