Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating

Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent...

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Autores principales:	Young Eun Hwang, Seonghun Im, Hyun Kim, Jung-Hoon Sohn, Byung-Kwan Cho, Ju Hyun Cho, Bong Hyun Sung, Sun Chang Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antimicrobial peptide coating infection L-DOPA in vivo bactericidal activity Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0b21581c72614f3bbc05b751f618c818
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spelling	oai:doaj.org-article:0b21581c72614f3bbc05b751f618c8182021-11-11T17:19:48ZAdhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating10.3390/ijms2221119151422-00671661-6596https://doaj.org/article/0b21581c72614f3bbc05b751f618c8182021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11915https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA<sub>5</sub>-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Staphylococcus aureus</i> within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA<sub>5</sub>-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.Young Eun HwangSeonghun ImHyun KimJung-Hoon SohnByung-Kwan ChoJu Hyun ChoBong Hyun SungSun Chang KimMDPI AGarticleantimicrobial peptidecoatinginfectionL-DOPAin vivo bactericidal activityBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11915, p 11915 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial peptide coating infection L-DOPA in vivo bactericidal activity Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	antimicrobial peptide coating infection L-DOPA in vivo bactericidal activity Biology (General) QH301-705.5 Chemistry QD1-999 Young Eun Hwang Seonghun Im Hyun Kim Jung-Hoon Sohn Byung-Kwan Cho Ju Hyun Cho Bong Hyun Sung Sun Chang Kim Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
description	Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA<sub>5</sub>-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, and <i>Staphylococcus aureus</i> within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA<sub>5</sub>-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.
format	article
author	Young Eun Hwang Seonghun Im Hyun Kim Jung-Hoon Sohn Byung-Kwan Cho Ju Hyun Cho Bong Hyun Sung Sun Chang Kim
author_facet	Young Eun Hwang Seonghun Im Hyun Kim Jung-Hoon Sohn Byung-Kwan Cho Ju Hyun Cho Bong Hyun Sung Sun Chang Kim
author_sort	Young Eun Hwang
title	Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
title_short	Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
title_full	Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
title_fullStr	Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
title_full_unstemmed	Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating
title_sort	adhesive antimicrobial peptides containing 3,4-dihydroxy-l-phenylalanine residues for direct one-step surface coating
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0b21581c72614f3bbc05b751f618c818
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Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating

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