A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth.
The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV...
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2011
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oai:doaj.org-article:0b21f29d1b304c1ba3b9073bdf0367282021-11-18T06:49:41ZA nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth.1932-620310.1371/journal.pone.0022362https://doaj.org/article/0b21f29d1b304c1ba3b9073bdf0367282011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21814578/?tool=EBIhttps://doaj.org/toc/1932-6203The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV and its role in IHNV growth in host cells was investigated. Through transient transfection in RTG-2 cells of NV fused to green fluorescent protein (GFP), a nuclear localization of NV was demonstrated. Deletion analyses showed that the (32)EGDL(35) residues were essential for nuclear localization of NV protein, and fusion of these 4 amino acids to GFP directed its transport to the nucleus. We generated a recombinant IHNV, rIHNV-NV-ΔEGDL in which the (32)EGDL(35) was deleted from the NV. rIHNVs with wild-type NV (rIHNV-NV) or with the NV gene replaced with GFP (rIHNV-ΔNV-GFP) were used as controls. RTG-2 cells infected with rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL yielded 12- and 5-fold less infectious virion, respectively, than wild type rIHNV-infected cells at 48 h post-infection (p.i.). While treatment with poly I∶C at 24 h p.i. did not inhibit replication of wild-type rIHNVs, replication rates of rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL were inhibited by poly I∶C. In addition, both rIHNV-ΔNV and rIHNV-NV-ΔEGDL induced higher levels of expressions of both IFN1 and Mx1 than wild-type rIHNV. These data suggest that the IHNV NV may support the growth of IHNV through inhibition of the INF system and the amino acid residues of (32)EGDL(35) responsible for nuclear localization are important for the inhibitory activity of NV.Myeong Kyu ChoiChang Hoon MoonMyoung Seok KoUnn-Hwa LeeWha Ja ChoSeung Ju ChaJeong Wan DoGang Joon HeoSoo Geun JeongYoo Sik HahmAbdallah HarmacheMichel BremontGael KurathJeong Woo ParkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22362 (2011) |
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Medicine R Science Q |
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Medicine R Science Q Myeong Kyu Choi Chang Hoon Moon Myoung Seok Ko Unn-Hwa Lee Wha Ja Cho Seung Ju Cha Jeong Wan Do Gang Joon Heo Soo Geun Jeong Yoo Sik Hahm Abdallah Harmache Michel Bremont Gael Kurath Jeong Woo Park A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| description |
The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV and its role in IHNV growth in host cells was investigated. Through transient transfection in RTG-2 cells of NV fused to green fluorescent protein (GFP), a nuclear localization of NV was demonstrated. Deletion analyses showed that the (32)EGDL(35) residues were essential for nuclear localization of NV protein, and fusion of these 4 amino acids to GFP directed its transport to the nucleus. We generated a recombinant IHNV, rIHNV-NV-ΔEGDL in which the (32)EGDL(35) was deleted from the NV. rIHNVs with wild-type NV (rIHNV-NV) or with the NV gene replaced with GFP (rIHNV-ΔNV-GFP) were used as controls. RTG-2 cells infected with rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL yielded 12- and 5-fold less infectious virion, respectively, than wild type rIHNV-infected cells at 48 h post-infection (p.i.). While treatment with poly I∶C at 24 h p.i. did not inhibit replication of wild-type rIHNVs, replication rates of rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL were inhibited by poly I∶C. In addition, both rIHNV-ΔNV and rIHNV-NV-ΔEGDL induced higher levels of expressions of both IFN1 and Mx1 than wild-type rIHNV. These data suggest that the IHNV NV may support the growth of IHNV through inhibition of the INF system and the amino acid residues of (32)EGDL(35) responsible for nuclear localization are important for the inhibitory activity of NV. |
| format |
article |
| author |
Myeong Kyu Choi Chang Hoon Moon Myoung Seok Ko Unn-Hwa Lee Wha Ja Cho Seung Ju Cha Jeong Wan Do Gang Joon Heo Soo Geun Jeong Yoo Sik Hahm Abdallah Harmache Michel Bremont Gael Kurath Jeong Woo Park |
| author_facet |
Myeong Kyu Choi Chang Hoon Moon Myoung Seok Ko Unn-Hwa Lee Wha Ja Cho Seung Ju Cha Jeong Wan Do Gang Joon Heo Soo Geun Jeong Yoo Sik Hahm Abdallah Harmache Michel Bremont Gael Kurath Jeong Woo Park |
| author_sort |
Myeong Kyu Choi |
| title |
A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| title_short |
A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| title_full |
A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| title_fullStr |
A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| title_full_unstemmed |
A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth. |
| title_sort |
nuclear localization of the infectious haematopoietic necrosis virus nv protein is necessary for optimal viral growth. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/0b21f29d1b304c1ba3b9073bdf036728 |
| work_keys_str_mv |
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