MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS

Abstract. T-lymphocytes play an important role in elimination of tumor cells, in reactions of a transplant against graft and graft versus host disease, in slow-type hypersensitivity, and other reactions directed for maintenance of homeostasis. Along with CD3, an antigen-specific T-cellular receptor...

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Autores principales:	S. V. Khaidukov, A. V. Zurochka, V. A. Chereshnev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	flow cytometry t-lymphocytes t-cellular receptor αβ-тcr γδ-тcr Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0b2655ecea364a7da753ba71dd46b685
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spelling	oai:doaj.org-article:0b2655ecea364a7da753ba71dd46b6852021-11-18T08:03:38ZMULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS1563-06252313-741X10.15789/1563-0625-2008-2-3-115-124https://doaj.org/article/0b2655ecea364a7da753ba71dd46b6852014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/105https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. T-lymphocytes play an important role in elimination of tumor cells, in reactions of a transplant against graft and graft versus host disease, in slow-type hypersensitivity, and other reactions directed for maintenance of homeostasis. Along with CD3, an antigen-specific T-cellular receptor (TCR) is another common marker of T-cells. There are two types of TcR – αβ-TcR and γδ-TcR that differ in ontogenetic and functional properties. γδ-T-cells play a significant role in protection of organism against various types of infections, and determination of their amounts should be an integral part of the analysis of patients’ immune status. To these purposes, a multi-colour analysis shuld be used, applying the following combinations of monoclonal antibodies: CD3/CD4/CD8/CD45 and αβ-TcR/γδ-TcR/CD3/CD45. Multi-colour staining and multi-step gating allow of carrying out multiparametric analysis of peripheral blood with high accuracy and reliability. The proposed approach considerably facilitates interpretation of results obtained, and it allows of judging about immune system functioning in various pathological conditions.S. V. KhaidukovA. V. ZurochkaV. A. ChereshnevSPb RAACIarticleflow cytometryt-lymphocytest-cellular receptorαβ-тcrγδ-тcrImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 2-3, Pp 115-124 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	flow cytometry t-lymphocytes t-cellular receptor αβ-тcr γδ-тcr Immunologic diseases. Allergy RC581-607
spellingShingle	flow cytometry t-lymphocytes t-cellular receptor αβ-тcr γδ-тcr Immunologic diseases. Allergy RC581-607 S. V. Khaidukov A. V. Zurochka V. A. Chereshnev MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
description	Abstract. T-lymphocytes play an important role in elimination of tumor cells, in reactions of a transplant against graft and graft versus host disease, in slow-type hypersensitivity, and other reactions directed for maintenance of homeostasis. Along with CD3, an antigen-specific T-cellular receptor (TCR) is another common marker of T-cells. There are two types of TcR – αβ-TcR and γδ-TcR that differ in ontogenetic and functional properties. γδ-T-cells play a significant role in protection of organism against various types of infections, and determination of their amounts should be an integral part of the analysis of patients’ immune status. To these purposes, a multi-colour analysis shuld be used, applying the following combinations of monoclonal antibodies: CD3/CD4/CD8/CD45 and αβ-TcR/γδ-TcR/CD3/CD45. Multi-colour staining and multi-step gating allow of carrying out multiparametric analysis of peripheral blood with high accuracy and reliability. The proposed approach considerably facilitates interpretation of results obtained, and it allows of judging about immune system functioning in various pathological conditions.
format	article
author	S. V. Khaidukov A. V. Zurochka V. A. Chereshnev
author_facet	S. V. Khaidukov A. V. Zurochka V. A. Chereshnev
author_sort	S. V. Khaidukov
title	MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
title_short	MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
title_full	MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
title_fullStr	MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
title_full_unstemmed	MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS
title_sort	multi-colour cytometric analysis. identification of t lymphocytes and their subsets
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/0b2655ecea364a7da753ba71dd46b685
work_keys_str_mv	AT svkhaidukov multicolourcytometricanalysisidentificationoftlymphocytesandtheirsubsets AT avzurochka multicolourcytometricanalysisidentificationoftlymphocytesandtheirsubsets AT vachereshnev multicolourcytometricanalysisidentificationoftlymphocytesandtheirsubsets
_version_	1718422523658371072

MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS

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