Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection

Abstract Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chuanzhong Zhang, Xin Wang, Feng Zhang, Lidong Dong, Junjiang Wu, Qun Cheng, Dongyue Qi, Xiaofei Yan, Liangyu Jiang, Sujie Fan, Ninghui Li, Dongmei Li, Pengfei Xu, Shuzhen Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0b27d8fd6311406bbb2168b0e1069460
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0b27d8fd6311406bbb2168b0e1069460
record_format	dspace
spelling	oai:doaj.org-article:0b27d8fd6311406bbb2168b0e10694602021-12-02T12:31:55ZPhenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection10.1038/s41598-017-07832-22045-2322https://doaj.org/article/0b27d8fd6311406bbb2168b0e10694602017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07832-2https://doaj.org/toc/2045-2322Abstract Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecular mechanism of PAL in soybean in response to P. sojae is largely unclear. Here, we characterize a novel member of the soybean PAL gene family, GmPAL2.1, which is significantly induced by P. sojae. Overexpression and RNA interference analysis demonstrates that GmPAL2.1 enhances resistance to P. sojae in transgenic soybean plants. In addition, the PAL activity in GmPAL2.1-OX transgenic soybean is significantly higher than that of non-transgenic plants after infection with P. sojae, while that in GmPAL2.1-RNAi soybean plants is lower. Further analyses show that the daidzein, genistein and salicylic acid (SA) levels and the relative content of glyceollins are markedly increased in GmPAL2.1-OX transgenic soybean. Taken together, these results suggest the important role of GmPAL2.1 functioning as a positive regulator in the soybean response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, genistein and SA.Chuanzhong ZhangXin WangFeng ZhangLidong DongJunjiang WuQun ChengDongyue QiXiaofei YanLiangyu JiangSujie FanNinghui LiDongmei LiPengfei XuShuzhen ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chuanzhong Zhang Xin Wang Feng Zhang Lidong Dong Junjiang Wu Qun Cheng Dongyue Qi Xiaofei Yan Liangyu Jiang Sujie Fan Ninghui Li Dongmei Li Pengfei Xu Shuzhen Zhang Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
description	Abstract Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecular mechanism of PAL in soybean in response to P. sojae is largely unclear. Here, we characterize a novel member of the soybean PAL gene family, GmPAL2.1, which is significantly induced by P. sojae. Overexpression and RNA interference analysis demonstrates that GmPAL2.1 enhances resistance to P. sojae in transgenic soybean plants. In addition, the PAL activity in GmPAL2.1-OX transgenic soybean is significantly higher than that of non-transgenic plants after infection with P. sojae, while that in GmPAL2.1-RNAi soybean plants is lower. Further analyses show that the daidzein, genistein and salicylic acid (SA) levels and the relative content of glyceollins are markedly increased in GmPAL2.1-OX transgenic soybean. Taken together, these results suggest the important role of GmPAL2.1 functioning as a positive regulator in the soybean response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, genistein and SA.
format	article
author	Chuanzhong Zhang Xin Wang Feng Zhang Lidong Dong Junjiang Wu Qun Cheng Dongyue Qi Xiaofei Yan Liangyu Jiang Sujie Fan Ninghui Li Dongmei Li Pengfei Xu Shuzhen Zhang
author_facet	Chuanzhong Zhang Xin Wang Feng Zhang Lidong Dong Junjiang Wu Qun Cheng Dongyue Qi Xiaofei Yan Liangyu Jiang Sujie Fan Ninghui Li Dongmei Li Pengfei Xu Shuzhen Zhang
author_sort	Chuanzhong Zhang
title	Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
title_short	Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
title_full	Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
title_fullStr	Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
title_full_unstemmed	Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection
title_sort	phenylalanine ammonia-lyase2.1 contributes to the soybean response towards phytophthora sojae infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0b27d8fd6311406bbb2168b0e1069460
work_keys_str_mv	AT chuanzhongzhang phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT xinwang phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT fengzhang phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT lidongdong phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT junjiangwu phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT quncheng phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT dongyueqi phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT xiaofeiyan phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT liangyujiang phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT sujiefan phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT ninghuili phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT dongmeili phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT pengfeixu phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection AT shuzhenzhang phenylalanineammonialyase21contributestothesoybeanresponsetowardsphytophthorasojaeinfection
_version_	1718394199574839296

Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection

Ejemplares similares