Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation

Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, infl uences the process of...

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Autores principales:	Milošević Ivan, Radovanović Anita, Danilović Luković Jelena, Lužajić Božinovski Tijana, Sourice-Petit Sophie, Beck-Cormier Sarah, Guicheux Jerome, Branislav Vejnović, Kovačevič Filipović Milica
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	type 1 2 and 10 collagens trap dmp-1 hypertrophic chondrocytes offspring hypothyroidism Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/0b281b0116244543931bd7626f739011
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spelling	oai:doaj.org-article:0b281b0116244543931bd7626f7390112021-11-17T21:27:53ZEffect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation1820-744810.2478/acve-2018-0026https://doaj.org/article/0b281b0116244543931bd7626f7390112018-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0026https://doaj.org/toc/1820-7448Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, infl uences the process of offspring endochondral ossifi cation affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcifi ed cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.Milošević IvanRadovanović AnitaDanilović Luković JelenaLužajić Božinovski TijanaSourice-Petit SophieBeck-Cormier SarahGuicheux JeromeBranislav VejnovićKovačevič Filipović MilicaSciendoarticletype 12 and 10 collagenstrapdmp-1hypertrophic chondrocytesoffspring hypothyroidismVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 3, Pp 301-320 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 1 2 and 10 collagens trap dmp-1 hypertrophic chondrocytes offspring hypothyroidism Veterinary medicine SF600-1100
spellingShingle	type 1 2 and 10 collagens trap dmp-1 hypertrophic chondrocytes offspring hypothyroidism Veterinary medicine SF600-1100 Milošević Ivan Radovanović Anita Danilović Luković Jelena Lužajić Božinovski Tijana Sourice-Petit Sophie Beck-Cormier Sarah Guicheux Jerome Branislav Vejnović Kovačevič Filipović Milica Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
description	Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, infl uences the process of offspring endochondral ossifi cation affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcifi ed cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
format	article
author	Milošević Ivan Radovanović Anita Danilović Luković Jelena Lužajić Božinovski Tijana Sourice-Petit Sophie Beck-Cormier Sarah Guicheux Jerome Branislav Vejnović Kovačevič Filipović Milica
author_facet	Milošević Ivan Radovanović Anita Danilović Luković Jelena Lužajić Božinovski Tijana Sourice-Petit Sophie Beck-Cormier Sarah Guicheux Jerome Branislav Vejnović Kovačevič Filipović Milica
author_sort	Milošević Ivan
title	Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
title_short	Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
title_full	Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
title_fullStr	Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
title_full_unstemmed	Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation
title_sort	effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/0b281b0116244543931bd7626f739011
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Effect of Subclinical and Overt Form of Rat Maternal Hypothyroidism on Offspring Endochondral Bone Formation

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